Effects of chitosan nanoparticles on mice infected with Listeria monocytogenes
Subject Areas : Biotechnological Journal of Environmental Microbiology
Sara Kazemi Rad
1
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran)
Mirsassan Mirpour
2
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch Islamic Azad University, Lahijan, Iran)
Mehdi Assmar
3
(Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran)
Mohamad Reza Razavi
4
(Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran)
Keywords: Nanoparticles, BALB/c mice, Listeriosis, Antibacterial Properties,
Abstract :
Listeria monocytogenes is the cause of listeriosis, which has many complications, especially in pregnant women. Due to the antibiotic resistance of this bacterium, many attempts have been made to introduce different medicinal compounds, including nanoparticles based on biological compounds. This research aimed to examine the mechanism of the effect of chitosan nanoparticles on L. monocytogenes in vivo (in the body of a living organism). The standard strain of L. monocytogenes (ATCC 7644) was prepared and analyzed in the Day Hospital Laboratory (Iran). The bacteria were examined based on biochemical tests. Then, the antibacterial activity of concentrations of 4.88 to 5000 µg/mL of chitosan nanoparticles against L. monocytogenes standard (ATCC 7644) was calculated with the investigated methods and the lowest inhibitory and bactericidal concentrations (MIC and MBC, respectively). The effects of different nanoparticle concentrations and ampicillin in mice infected with bacteria were also investigated. In infected mice, the therapeutic effect increased with increasing the nanoparticle concentration, and the concentration of 156.25 /mL was the most effective compared to other treatments. Also, ampicillin chitosan nanoparticles with a concentration of 39.06 g/mL had almost the same therapeutic effect. With the timely identification of listeria contamination in pregnant women and the proper use of chitosan nanoparticles instead of common drugs, a new solution can be found for the treatment of listeriosis.