The Impact of Rhizospheric Pseudomonas aeruginosa on the Growth of Melissa officinalis

Subject Areas : Biotechnological Journal of Environmental Microbiology

Elham Karami ¹ , Monir Doudi ² , Zahra Rezayatmand ³ , Ladan Rahimzadeh Torabi ⁴

1 - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan , Iran
3 - Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
4 - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Received: 2023-09-03 Accepted : 2023-09-13 Published : 2022-10-01

Keywords: Plant growth, Pseudomonas aeruginosa, Melissa Officinalis, Rhizobacteria,

Abstract :

Microbial communities occupy a significant position in the functioning and productivity of agricultural ecosystems. The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually beneficial partnerships with different plants. The objective of this study was to ascertain and isolate Pseudomonas strains that were obtained from the rhizosphere soil of M. officinalis. Furthermore, an examination was conducted on the morphological characteristics of M. officinalis that had been treated with standard and rhizospheric Pseudomonas. The experimental treatments consisted of 3 different groups: a rhizosphere Pseudomonas inoculation with a concentration of 106 and 109 CFU /ml, standard Pseudomonas inoculation with a concentration of 106 and 109 CFU /ml, and a control group that did not contain any bacteria. To effectively separate bacteria, specialized culture mediums, such as the King B medium, were employed. The biochemical and molecular results revealed that the isolated strain was Pseudomonas aeruginosa. The standard Pseudomonas treatment with a concentration of 109 CFU/ml exhibited the greatest root length of 24.1 cm, while the treatment lacking the presence of Pseudomonas displayed the lowest root length of 12.9. Treatments with rhizospheric P. aeruginosa showed the greatest values for stem length, as well as root and stem weight. Meanwhile, the control treatment resulted in the lowest measurements for these mentioned characteristics.