Antimicrobial activity of Ethanolic Extracts of (Tragopogon Collinus L. and Allium Eriophyllum L.) on Some Selected Environmental and Pathogenic Bacteria

Subject Areas : Biotechnological Journal of Environmental Microbiology

Ehsan Abolfathi ¹ (Department of Microbiology and Immunology, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran)
Nour Amirmozafari ² (Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran)
Ezaat Ghaemi ³ (Department of Medical Microbiology, Golestan University of Medical Sciences, Gorgan, Iran.)

Received: 2022-08-29 Accepted : 2022-09-24 Published : 2022-03-01

Keywords: ٍEthanolic extract, Antibacterial activity, Tragopogon collinus L, Allium eriophyllum L,

Abstract :

Due to the increase in the incidence of infectious diseases and the prevalence of antibiotic resistance in bacteria. The present study aimed to determine the antibacterial activity of the ethanolic extracts of the edible plants Tragopogon collinus L. and Allium eriophyllum L. against some environmental and pathogenic bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Enterococcus faecalis. The extraction of medicinal plants was conducted using ethanol and the percolation method. Agar well diffusion was used to determine the antimicrobial effects of the extracts. The ethanolic extract of Tragopogon collinus showed an inhibitory effect on all bacteria in 250 µg/ml concentration, and the greatest effect was observed on Staphylococcus aureus and E. faecalis, with 20 and 18 mm diameter of zone, respectively. In the 125 µg/ml concentration, with a 21 mm diameter of zone, it had the highest antimicrobial effect on Klebsiella pneumoniae, compared with other bacteria. Regarding the ethanolic extract of Allium eriophyllum L., the highest antibacterial effect at 250 µg/ml concentration was 20 and 22 mm zone diameters. The effect of this plant extract on Pseudomonas aeruginosa was lower than its effect on other bacteria. The MIC of the extract of Tragopogon collinus L. was found at a 5.75 µg/ml concentration on staphylococcus aureus and E. faecalis. The MIC of the ethanolic extract of Allium eriophyllum L. was found at an 11.5 µg/ml concentration on staphylococcus aureus, pseudomonas aeruginosa, and Klebsiella pneumoniae. The results of this study indicate that Tragopogon collinus and Allium eriophyllum have antibacterial properties.