Effect of Alpha-Hydroxycholecalciferol and Phytase Enzyme on Growth Performance, Bone Characteristics and Intestinal Morphology in Broiler Chickens
Subject Areas : Journal of Animal BiologyArezoo Habibi 1 , Nima Eila 2 * , Mohammad Reza Paryani 3 , Abolfazl Zarei 4
1 - Department of Animal Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran
2 - Department of Animal Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Animal Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran
4 - Department of Animal Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran
Keywords: Alpha-hydroxycholecalciferol, Phytase, Growth, Broiler chicken,
Abstract :
The aim of the present experiment was to investigate the effect of alpha-hydroxycholecalciferol and phytase on growth performance, bone characteristics, and intestinal morphology in broiler chickens. To conduct the experiment, 400 one-day-old male chicks of the Cobb 500 strain were used in a completely randomized design with 4 treatments and 5 replications (20 chicks per replication). The experimental treatments included 1- control (no additive), 2- control + 14 mg/kg alpha-hydroxycholecalciferol, 3- control + 5 mg/kg phytase, 4- control + 14 mg/kg alpha-hydroxycholecalciferol + 5 mg/kg phytase. According to the results, the weight gains of chickens in all rearing periods in treatments that used alpha-hydroxycholecalciferol alone and also together with phytase increased significantly compared to the control (p < 0.05). The diameter of Haversian ducts in the phytase-containing treatment significantly increased compared to the control (p < 0.05). The length of ileum villus in treatments containing alpha-hydroxycholecalciferol and its mixture with phytase significantly increased compared to the control (p < 0.01). Overall, the results showed that the use of alpha-hydroxycholecalciferol together with phytase had a better effect on improving growth performance, bone strength, and intestinal tissue compared to their separate use in broiler diets.
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