Compare the Toxicity of Silver Nanoparticles Produced by Biological and Chemical Methods on Artemia franciscana in Naupliar and Adult Stages
Subject Areas : Journal of Animal BiologyS. Mashjoor 1 , M. Alishahi 2 , Z. Tulaby Dezfuly 3
1 - Department of Marine biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Keywords: Toxicity, silver nanoparticles, Artemia fransiscana, Marine macroalgae,
Abstract :
In this study, the toxicity of silver nanoparticles (AgNPs) and the difference in sensitivities in naupliar and adult stages of Artemia fransiscana, were evaluated to absorption of chemical and biosynthesized by the seaweed Ulva flexuosa, forms of AgNPs. The A. nauplii and adult were exposed to additive serial concentration of biosynthetic and chemical AgNPs. Mortality in each group at the times of 12, 24, 36 and 48 h after exposure to AgNPs were recorded and analyzed via Probit software. The toxicity of these two types of AgNPs on A. nauplii and adult increased in a dose-dependent manner, but also their toxicity increased along with duration of exposure time. The toxicity of nanoparticles were significantly different (P<0.05). Therefore, after 48 h, the LC50 value of chemical AgNPs in the A. nauplii was 31.8 mg/l, while 48 h LC50 for biosynthesis AgNPs was 366.96 mg/l. While the concentration of the chemical AgNPs have assessed in adults artemia during exposure was 47 mg/l and for biosynthesis form of AgNPs was 240 mg/l. The chemical form of AgNPs was more toxic potential than a biosynthetic form of its. The sensitivity of A. nauplii to chemical AgNPs was more than to mature stage, but compared to biosynthesis form was less than adult. This research could be pioneer to the development of biocompatible functionality of nanoparticles with eco-management objectives.
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