Protective Effects of Chicory (Cichorium intybus) Hydroalcoholic Extract on Hepatotoxicity of Vincristine Sulfate in Rats

Subject Areas : Journal of Animal Biology

Neda Dehasht ¹ , Bostan Roudi ² , Vida Hojati ³

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Received: 2019-05-10 Accepted : 2019-09-05 Published : 2021-02-19

Keywords: liver enzymes, chicory, Rat, Vincristine,

Abstract :

Vincristine is an anti-cancer drug which fights the growth and spread of cancer cells in the body. This study aimed to investigate the protective effects of hydroalcoholic extract of chicory plant (Cichorium intybus)on hepatotoxicity of vincristine sulfate in rats. Adult male Wistar rats were used in this study. 56 mice were divided into 7 groups of eight. Treatments were intraperitoneally performed for 15 days. The control group contained healthy mice. Experimental groups 1 and 2, each contains three subgroups with defined concentrations: Experimental group a1 received 15 mg/kg of chicory extract, 0.05 mg/kg of vincristine sulfate and again chicory extract. Experimental group b1 received 20 mg/kg of chicory extract, 0.1 mg/kg of vincristine and chicory extract again. Experimental group c1 received 25 mg/kg of chicory extract; then, vincristine at a dose of 0.2 mg/kg, and then chicory extract again. Experimental group a2: first 0.5 mg/kg vincristine then chicory extract at 15 mg/kg dose, and received Wayne Christian. Experimental b2: First, they received 0.1 mg/kg of vincristine; then, 15 mg/kg of chicory extract and again of vincristine. Experimental c2: first, they received 0.2 mg/kg vincristine, then 15 mg/kg chicory extract, and again vincristine. After performing the above steps by anesthetizing the animals, blood sampling from mice, serum isolation, liver isolation, the measurement of the level of biochemical parameters, and liver enzymes, and their liver tissue were tested. In this study, a significant increase in ALT and AST enzymes was observed in experimental groups 1 compared to the control group, and also a significant decrease was observed in experimental groups 2 compared to experimental groups 1. The results showed that the subcutaneous injection of vincristine had additive effects on the number of liver enzymes in experimental group 1, the chicory extract had high antioxidant properties and significantly reduced liver enzymes.

References:

1. Asadi-Noghabi A.A., Heshmati P. 1999.Comprehensive culture of generic and herbal medicines of Iran along with nursing-medical measures and dental considerations. Andisheh Rafi Publication, 1008 pp.


2. Berger AR, shaumburg H. 1994. Disorders of peripheral nervous system in : Textbook of clinical occupational and environmental medicine. 5th ed, Philadelphia, WB Sanders Co, 1994: 482-583.


3. Bice F., Andera H. 2003. Role of reactive oxygen species in lung injury and diseases. Free Radical Biology and Medicine, 34: 1507-1516.


4. Figge J., Rossing T.H., Fend V.J. 1991. The role of serum proteins in acid –base equilibria. The Journal of Laboratory and Clinical Medicine, 117: 453-467.


5. Katti S.R., Sathyanesan A.G. 1986. Effects of captopil on membrane-associated enzymes in lead-induced hapatotoxicity in rats. Neurotoxicology,7:74-52


6. Kashiwagi A., Asahina T., Ikebuchi M., 1994. Abnormal glutathione metabolism and increased cytotoxicity caused by H2O2 in human umbilical vein endothelial cells cultured in high glucose medium. Diabetologia, 37: 264–269.

Khalil H.A., Belal T.S., El-Yazbi A.F., Hamdy D.A. 2016.The effect of increased lipoproteins levels on the disposition of vincristine in rat. Lipids in Healthand Disease, 15(1): 152.

1. Kobayashi T., Robinson J.M., Segachi H., 1998. Identification of intracellular sites of suproxide production in stimulated neutrophils. Journal of Cell Science, 111: 8-91.


2. Limdi J., Hyde G. 2003. Evaluation of abnormal liver function tests. Postgrade Medical Journal, 79: 307-312.


3. Lund B.A, Miler, D.M., Woods J.S. 1991. Mercury–induced H2o2 Formation and lipid Peroxidation in vitro in rat kidney mitoch on dria. Biochemistry and Pharmacology, 32: 181-187.


4. Magnsson I., Persson R.G., Page R.C. 1996. Amulti – centerclinical trial of a new chair side test in distinguishing between diseased and healthy periodontal sites II. Association between site type and test outcome before and after the rapy. Journal of Periodontology, 67: 589-96.


5. Michiels C., Raes M., Toussaint O., Remacle J. 1994. Importance of Seglutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radical Biology and Medicine, 17: 235–248.


6. Moloudi M.R., Hosseinzadeh K. Rohani Sh., Zandi F., Ahmadi A., Khalvatian P., Rostami A., Sheikhesmaeili F., Izadpanah E. 2009. The effect of chicory extract of chicory plant on liver function indices and serum TNF-α level in rat obstructive cholestasis model. Journal of Kordestan University of Medical Sciences, 19(4): 10-19.


7. Molzemi S. 2015. Review gavage Ritalin on blood albumin and some liver enzymes. Govaresh Journal, 20(4): 237-242.


8. Molzemi S., Bolbol Haghighi N., Haratipor H., Molzemi Sh., Alamal-Hoda F., Ashenaii M.H., Mousavi S.R., Jafari M.R., HassanZade A., Sedighi M. 2015., Survey on Intra-Peritoneal Injection of Mercuric Chloride on Blood Albumin and Liver Enzymes, Govaresh Journal, 19(4): 236-241.


9. Oringer RJ, Howell TH, Ne vins ML, Reasner DS, Davis GH, se kler J, Fiorellini JP. 2001. Relationship between crevicular aspartate aminotransferase levels and periodontal disease progression. Journal of Periodontology, 72: 17-24.


10. Ozeki Y., Kurono Y. 1994. Effects of drug bin ding on the sterase activity of human serum albumin: inhibition modes and binding sites of anionic drugs. Chem. Pharm. Bulletin, 28: 535-540.


11. Peinharaf J.W. 1992. Side-effect: mercury contribution to body burden from dental amalgam. Advanced Dental Research, 6: 110-113.


12. Savafian, T.A., 1999. Methylmercury induced generation of free radicals: biological implications. Metal Ions in Biological Systems, 36: 415-444.


13. Shimada K., Mizuno T., Ohshio K., Kamaga M., Murai S., Ito K. 2000. Analysis of aspartate aminotransferase in gin gival crevicular fluid assessed by asing pocket watch: alongitudinal study with initial therapy. Journal of Clinical Periodontology, 27(11): 819-823.


14. Strubelt O., Hunter TC. 1996. Comparative studies the toxicity perfused rat liver. Journal of Toxicology and Environmental Health, 47(3): 267-283.