An Investigation into the Effect of Nigella Sativa Oil on the Treatment of Gastric Ulcer Induced by Acetic Acid in Male Rats

Subject Areas : Journal of Animal Biology

Mahdieh Azarshab ¹ (Biology Department, Tehran North Branch, Islamic Azad University, Tehran, Iran)

Received: 2018-10-15 Accepted : 2019-04-03 Published : 2019-05-22

Keywords: Nigella sativa, Rat, acetic acid, Gastric ulcer, Linoleic oil,

Abstract :

Gastric ulcer or gastritis is a benign disease in the digestive system which is caused by a small ulcer in the inner lining and gastric mucosa. Among gastric ulcer characteristics is the deep ulcer that passes through the gastric mucosa. Almost all benign gastric ulcers are located in the anthrome (the lower stomach area). One of the methods to treat gastric ulcer is the use of medicinal herbs. Among effective medicinal herbs in treating gastric ulcer is Nigella sativa. The most important application of Nigella sativa is in inflammation reduction and wound healing acceleration. Substances such as linoleic oil, thymoquinone and antioxidants are obtained from nigella sativa. The rats were fed using a standard laboratory diet. Prior to surgery, the rats were deprived of food for 48 hours, and no water was given to them two hours before surgery. Gastric ulcer was generated by injecting acetic acid to stomach lining where there were no vessels. The oil at different doses was given to rats through gavage tube one day after gastric ulcer was generated. The rats were killed on days 4, 7, 10, and 14, and their stomachs were removed and the ulcer dimensions were calculated in terms of m2. Moreover, after cutting and providing lam, in order to conduct histological studies, samples were stained using hematoxylin and eosin and were examined by microscope. The obtained results showed that the percentage of wound healing in Nigella sativa at three doses (50, 100, 200 mg/kg) on days 4, 7, 10, and 14 had a significant increase in comparison to sham-operated and control groups (P<5%). Due to the presence of compounds such as terpenes, flavonoids, vitamins A, B, and C, and the anti-inflammatory and antioxidant properties in Nigella sativa, it facilitates the gastric ulcer healing, protects stomach tissue, and reduces gastric inflammation and ulcer, and has a greater restorative effect on gastric ulcer caused by acetic acid.

References:

1. Akah P.A., Orisakwe O.E., Gamaniel K.S., Shittu A., 1998. Evaluation of Nigerian traditional medicinits effect of some Nigerian flook remedies on peptic ulcer. Journal of Ethnopharmacology, 62(2): 123-127.


2. Alkofahi A., Atta H.A., 1999. Pharmacological screening of anti-ulcerogenic effect of some Jordanian medicinal plants in rats. Journal of Ethnopharmacology, 67(3): 341-345.


3. Asqari S., Moshtayan J., Hoseini M., Naderi G., 2008. Survey of effects of using nigella sativa on white globule of rabbits. Journal of Birjand University of Medical Sciences, 21: 460-464.


4. Awaad A.S., EI-Meligy R.M., Soliman G.A., 2012.  Natural products in treatment of ulcerative colitis and peptic ulcer. Journal of Saudi Chemical Society, 17(1): 101-124.


5. Berg R.A., Steinmann B., 2003. Ascorbate deficiency results in decreased collagen production: under-hydroxylation of praline lead to increased intracellular degradation. Archives of Biochemistry and Biophysics, 22(6): 681-686.


6. Cavin C., Delannoy M., Malnoe A., 2005. Inhibition of the expression and activity of cyclooxygenase-2 by chicory extract. Biochemical and Biophysical Research Communications, 327(7): 742-749.


7. Changizian F., 2012. Ultimate general pharmacy guide. Tehran: Niloufaraneh publication, 8th edition, Vol. 1, pp. 213.


8. Davidson J., 2001. Rabbit model of wound healing. ETRS Annual Conference, 31: 307-317.


9. Doustmohammadi M., 2010. Survey of antimicrobial effects of malva juice and nano-    crystalline silver particles on Staphylococcus aureus, enterococcus faecalis pseudomonas aeruginosa and salmonella typhimurium in laboratory situation and animal model. MSc. thesis in Microbiology, Islamic Azad University of Zanjan, pp. 23-30.


10. Elisabetsky E., Castilhos Z.C., 1990. Plants used as analgesics by Amazonian caboclos as a basis for selecting plants for investigation. International Journal of Crude Drug Research, 28(4): 309-320.


11. Filippo D’Antuono L., Moretti A., 2002. Lovato A.F.S., Seed yield, yield components, oil content and essential oil content and composition of Nigella sativa L. and Nigella damascena L., Industrial Crops and Products, 15: 59-69.


12. Goreja W.G., 2003. Black seed, natures miracle remedy. New York: Amazing Herbs Press, pp. 33-46.


13. Houghton P.I., Zarka R., De las Heras B., Hoult R.S, 1995. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Medica, 61: 33-36.


14. Medline Plus. 2014. Peptic ulcer. National Institutes of Health,2: 68-84.


15. Meral I., Yener Z., Kahraman T., Mert N., 2001. Effect of Nigella sativa on glucose concentration, lipid peroxidation, anti-oxidant defense system and liver damage in experimentally-induced diabetic rabbits. Journal of Veterinary Medicine Series A- Physiology, Pathology, Clinical Medicine, 48: 593-599.


16.  Mousavi F., Rahnema M., 2011. Survey of effect of indigenous Lactobacillus pentosus of Iran on healing the gastric ulcer of male rats.MSc thesis of Microbiology, Islamic Azad University of Zanjan, pp. 58.


17. Pushparaj P., Low H.K., Manikanada M., 2007. Antidiabetic effects of Cichorium intybus L. in streptozotocininduced diabetic rats. Journal of Ethnopharmacology, 111(2): 430-434.


18. Ramakrishnan K., Robert C., Salinas M.D., 2007. Peptic ulcer disease. Oklahoma Health Sciences, 2: 1006-1012.


19. Roughani M., Balouchnezhad T., Dehkordi R., 2007. Survey of effect of dietary administration of hypoglycemic and hypoglycemic and long-term administration of nigella sativa in diabetic rats. Journal of Guilan University of Medical Sciences, 16: 26-31.


20. Sokouti A., 2011. Effect of Lactobacillus bacteria on healing of gastric ulcer caused by acetic acid in male rats; wistar race. MA thesis of Microbiology, Islamic Azad University of Zanjan, pp. 78.


21. Stanojkovik L., Petrovic J., Comic L., 2004. Antibacterial activity of Cichorum intybus L. Fitoterapia, 75(8): 737-739.


22. Vanwijck R., 2001. Surgical biology of wound healing. Bulletin of Belgium Royal Academy of Medicine Membership, 56(3-4): 175-184.


23. Watson G.D., Smith R., 2005. Gastrointestinal nursing. Blackwell:  Oxford.


24. Worthen D.R., Ghosheh O.A., Crooks P.A., 1998. The in vitro anti-tumor activity of some crude and purified components of black seed, Nigella sativa L. Anticancer Research, 18: 1527-1532.

Zargari A., 1987. Medicinal Herbs, 4th edition, Tehran University Publication, Tehran, Iran, Vol. 1, pp. 4-34.