The Study of Histology and Histometry Changes in the Stomach and Intestine of Male Mice Consuming Nano-Styrene Microplastics

Subject Areas : Journal of Animal Biology

Mohammad Reza Ghafari Manesh ¹ , Esmail Fattahi ² , Fereshteh Mirmohammadrezaei ³ , Sima Mashayekh ⁴

1 - Department of Biology, Ayatolla Amoli Branch, Islamic Azad University, Amol, Iran
2 - Department of Biology, Ayatolla Amoli Branch, Islamic Azad University, Amol, Iran
3 - Animal Biology Department, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
4 - Department of Biology, Ayatolla Amoli Branch, Islamic Azad University, Amol, Iran

Received: 2023-12-13 Accepted : 2024-01-20 Published : 2024-03-05

Keywords: Microplastic, Nano-styrene, Histology, Stomach, Intestine,

Abstract :

Today, microplastics are among the most important environmental pollutants that have attracted the attention of scientists. These plastic particles enter the food chain by being swallowed by animals and cause serious damage to humans and animals. The purpose of this research is to investigate the effect of polystyrene microplastics on the digestive system of mammals. The rats were divided into 3 groups of 6; the sham group received 200 microliters of distilled water once a day for 30 days. The first group received 200 microliters of nano-styrene once a day with a dose of 0.1mg⁄kg for 30 days. The second group received 200 microliters of nano-styrene once a day. He received a dose of 0.5 mg⁄kg for 30 days. The day after the last gavage, the animal was anesthetized with chloroform, and the intestinal and stomach samples were stained for histological examination in 10% buffered formalin solution with hematoxylin-eosin staining method. TUKEY test was used for statistical analysis and comparison between groups. All the calculations were done using Prism software and the significance level of the tests P was considered less than 0.05. The statistical findings show a decrease in the diameter of the submucosa, the number of lining cells, and the number of epithelial cells in the experimental group 2 compared to the control group. The height of the intestinal villi and The stomach showed an increase in experimental group 2 compared to the sham group. The results of this study show that the effects of nano-styrene depend on the concentration and as the concentration increases, it will leave more destructive effects on the intestines and stomach.

 

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