Molecular Analysis of Najdi Goat Population Using HVR1 Sequence of Mitochondrial Genome

Subject Areas : Journal of Animal Biology

Rouhollah Khademi ¹ , Seyedeh Ommolbanin Ghasemian ^{2 *} , Hamid Reza Seyyed Abadi ³ , amin kazemizadeh ⁴

1 - Department of Animal Sciences, Behbahan Branch, Islamic Azad University, Behbahan, Iran
2 - Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran
3 - Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4 - Animal Science Research Department, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran

Received: 2023-10-17 Accepted : 2023-11-22 Published : 2024-01-05

Keywords: Mitochondrial genome, HVR1 region, DNA sequencing, phylogeny, Najdi goat,

Abstract :

This study was conducted to determine the sequence of the HVR1 region of the mitochondrial genome of the Najdi goat. To conduct this study, 30 blood samples of both gender were collected from unrelated goats. After DNA extraction, the desired region was amplified by specific primers by PCR technique, and the sequence was determined. For comparison, the phylogeny of the HVR1 region sequence obtained from Najdi goat was drawn with other breeds worldwide to determine the haplotype group. The phylogenetic tree drawn for the samples showed that they all originated from the same population and the number of 5 different haplotypes was determined based on 20 nucleotide polymorphisms (SNP) for the HVR1 region in the sequences. Also, the sequence of the HVR1 region of the studied sample with 11 sequences recorded from 6 haplotype groups from different countries in the NCBI database showed that the Najdi goat belongs to haplotype group A. Comparing the sequence of the HVR1 region with the sequences in the gene bank can contribute to our information about the Najdi goat breeds and open the ground for their better use in breeding programs. According to the obtained results, the genetic diversity of the Najdi goat has increased over many years, and this increase in genetic diversity can be due to the mixing of this breed with other breeds, which can lead to the extinction of the Najdi goat in the future, which requires more attention to this issue.

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