The Frequency and Antibiotic Resistance Pattern of Aeromonas hydrophila and Yersinia ruckeri Isolated from Diseased Rainbow Trout Cultured in Chaharmahal and Bakhtiari Province

Subject Areas : Journal of Animal Biology

Nima Panahi Far ¹ , Mohsen Pournia ² , Reza Salighehzadeh ^{3 *} , Marjan Mosafer ⁴

1 - Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
2 - Department of Microbiology, Masjed-Soleiman Branch, Islamic Azad University, Masjed-Soleiman, Iran
3 - Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
4 - Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2022-09-14 Accepted : 2023-01-08 Published : 2023-08-23

Keywords: Antibiotic susceptibility, Aeromonas hydrophila, Oncorhynchus mykiss, Chaharmahal and Bakhtiari province, Yersinia ruckeri,

Abstract :

Recognition of resistance and susceptibility of problematic bacteria such as Yersinia ruckeri and Aeromonas hydrophila in rainbow trout farms, in proper selection of antibiotics and infection control in breeding ponds. It plays an effective role. In this study, 100 diseased fish were collected from rainbow trout farms in Chaharmahal and Bakhtiari province and after performing biochemical tests and purification of Aeromonas hydrophila and Yersinia ruckeri species, using PCR test with specific primers were finally approved. Antibiotic susceptibility of bacteria was investigated by disk diffusion method by culture on Müller-Hinton agar medium. Examination of the results of antibiogram of Aeromonas hydrophila isolates showed that the highest resistance was related to the antibiotics ampicillin (100%), colistin (80%), enrofloxacin (80%), flumquin (80%) and then, Compared to tetracycline (60%) and fluorophenicol (60%) antibiotics. The most sensitive isolates were related to trimethoprim-sulfamethoxazole (60%) and phosphomycin (60%) antibiotics. Examination of the antibiogram results of Yersinia ruckeri isolates showed that the highest resistance among the isolates was related to the antibiotics ampicillin (100%), erythromycin (100), trimethoprim-sulfamethoxazole (100%) and colistin (83.34%) and then showed about 67% resistance to tetracycline, fluorophenicol and phosphomycin antibiotics. Resistance to the antibiotics flumequin, lincomycin + spectinomycin (lincospectin) and enrofloxacin were also observed to be 50%, which means that none of these antibiotics would be a viable option for the treatment of bacterial infections caused by Yersinia ruckeri. In order to avoid growing bacterial resistance and to take the most effective decision in terms of antibiotic therapy, usage of antibiotics without antibiogram must be avoided. .

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