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Manuscript ID : ASCIJ-2301-1461 (R1) Visit : 299 Page: 133 - 146

10.22034/ascij.2023.1978481.1461

Article Type: Original Research

The Effect of Intraperitoneal Injection of Carvacrol Administration on Lipopolysaccharides-Induced Renal Oxidative Stress in Male Rats

Subject Areas : Journal of Animal Biology

Alireza Mortazavi 1 (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Hossain Mohammad Pourkargar 2 (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Farimah Beheshti 3 (Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran|Department of Physiology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran)
Gholamhasan Vaezi 4 (Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Mahmoud Hosseini 5 (Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran)

Received: 2022-09-14 Accepted : 2023-01-08 Published : 2023-08-23

Keywords: Carvacrol, Oxidative stress, Lipopolysaccharide, IL-1β, Acute Kidney Injury,

Abstract :

Carvacrol is a natural monoterpene phenol which retains significant antibacterial, antifungal, anti-cancer and anti-inflammatory activity. However, the effect of carvacrol on oxidative stress in lipopolysaccharide-induced acute kidney injury has not yet been reported. The present study aimed to investigate the protective effects of carvacrol on lipopolysaccharides-induced renal oxidative stress and inflammatory responses in male rats. Male Wistar rats (weighing 200 to 250 g, n=7 each group) were divided into the following groups: 1) control, 2) LPS, 3) LPS+carvacrol 25 mg/kg, 4) LPS+carvacrol 50 mg/kg and 5) LPS+carvacrol 100 mg/kg. For inducing acute kidney injury, 1 mg/kg LPS was injected intraperitoneally for 2 weeks. Carvacrol was administered intraperitoneally 30 minutes prior LPS injection. After preparation of renal homogenates, levels of inflammatory cytokines IL-1β and oxidative stress indexes (malondialdehyde, total thiol, catalase and Superoxide dismutase activity) were detected by ELISA and spectrophotometric methods, respectively. Our results showed that LPS injection increased malondialdehyde and IL-1β while, it reduced total thiol, catalase and SOD activity in the renal tissue. But, pretreatment with carvacrol not only decreased malondialdehyde and IL-1β levels but also increased total thiol, catalase and SOD activity. Simple regression analysis revealed that MDA was positively correlated with IL-1β level). However, there was a significant negative correlation between MDA level and total thiol, SOD and CAT activities. Our results showed that carvacrol protects the kidney by reducing IL-1β and preventing oxidative stress in the renal tissue.enal tissue.

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