Evaluation of Antibody Titer from Newcastle Vaccine and Histological Effect in Sebright Chickens

Subject Areas : Journal of Animal Biology

Kimia  Karimi Sani ¹ (Department of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran)
Mehdi  Rezaei ² (Department of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran)
Mohammadreza  Hossenchi ³ (Department of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran)

Received: 2023-05-02 Accepted : 2023-07-25 Published : 2024-06-01

Keywords: antibody titer, Histopathology, Newcastle, sebright chicken,

Abstract :

Newcastle disease (ND) is one of the most important viral disease which make lots of casualty in poultry flocks. Therefor vaccination against ND is the main way to prevents the damages. The aim of this research was evaluation of antibody titer from ND vaccines and histopathological effects in Sebright chickens. In this research, 72 one-day-old Sebright chickens were tested in a completely random format in two groups (with three replications). From day one to the end of period, the breeding conditions were the same for all quails and the differences between the groups were only in the Newcastle disease vaccination program. Vaccination in the first group was based on using: Clone ND vaccine in day old (eye drop), injection of ND/AI + Clone ND (eye drop) in day 8 and using Avinew vaccine (drinking water) in day 15. The second group was considered as the control group (without vaccination). Blood samples were taken at day 25 and 35 and evaluated by HI test. Also, a sample was taken from the intestine and a histopathology specimen was prepared. The results of statistical analysis by Tukey test showed that the mean titer of Newcastle antibody in the vaccinated groups was significantly different (p < 0.01). Also, the results of morphometry and morphology of Newcastle vaccine in the intestinal tissue in the vaccinated groups showed a significant difference compared to the control group (p < 0.05). This study presents as a strategy in terms of antibody production and histological effects after vaccination in Sebright farms.

References:

1.Alexander D.J. 2000. Newcastle disease and other avian paramyxoviruses. Revue Scientifique et Technique, 19(2):443-462.

2. Alexander D.J., Senne D.A. 2008. A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. 5th ed. American Association of Avian Pathologists, pp:135-141.


3. Alexander D J., Jones R C. 2008. Poultry Diseases. 6th ed. Saunders ELSEVIER. pp: 294-305.


4. Butcher G.D., Mile R.D. 2018. The Avian Immune System. Florida, Ifas Extension University of Florida, 74:1-2.


5. 5. Ekarius C. 2016. Poultry Breeds: Chickens, Ducks, Geese, Turkeys: The pocket Guide to 104 Essential Breeds. Storey Publishing, LLC.


6. Erf GF. 2004. Cell-mediated immunity in poultry. Poultry Science, 83(4):580-590.

7.Ghalyanchi Langeroudi A., Hossein H., Karimi V., Madadgar O., Hashemzadeh M., Ghafouri S.A., Bagheri S.S., Vahedi S.M. 2014. 'Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012', Iranian Journal of Veterinary Medicine, 8(2):73-77.

8. Hussein E.A., Hair-Bejo M., Adamu L.A.R., Omar A.R., Arshad S.S., Awad E.A., Aini I. 2018. Scoring System for LesionsInduced by Different Strains of Newcastle Disease Virus in Chicken. Veterinary Medicine International, (1):9.


9. Kapczynski D.R., Afonso C.L., Miller P.J. 2013. Immune responses of poultry to Newcastle disease virus. Developmental and Comparative Immunology, 41(3):447-453.


10. Khader M.A., El-Kady M.F., Shaheed I.B. 2021. Comparative study between the post vaccinal histopathological alterations in some broiler chickens’ tissues induced by lasota and vg/ga newcastle disease vaccinal strains. Advances in Animal and Veterinary Sciences, 9(8):1135-1142.


11. Leslie J. 2000. Newcastle disease: Outbreak losses and control policy costs. Veterinary Record, 146(2):603-606


12. Mohammadamin O.G., Qubih T.S. 2011. Histopathology of virulent Newcastle disease virus in immune broiler chickens treated with IMBO®. Iraqi Journal of Veterinary Sciences, 25(1):9-13.


13. Miller P.J., Afonso C.L., El Attrache J., Dorsey K.M., Courtney S.C., Guo Z., Kapczynski D.R. 2013. Effects of Newcastle Disease Virus Vaccine Antibodies on the Shedding and Transmission of Challenge Viruses, Developmental and Comparative Immunology, 41(4):505-513.


14. Perozo F., Marcano R, Afonso C.L., 2012. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. Journal of Clinical Microbiology, 50(4): 1204-1208.


15. Perozo F., Villegas P., Dolz R., Afonso C.L., Purvis L.B. 2008. The VG/GA strain of Newcastle disease virus: mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathology, 37(3):237-245.


16. Ratih D., Handharyani E., Setiyaningsih S. 2017. Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia. Veterinary World, 10(9):1066-1071


17. Roohani K., Tan S.W., Yeap S.K., Ideris A., Bejo M.H., Omar A.R. 2015. Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV. Journal of veterinary Science, 16(4), 447–457.


18. Samadi S., Kianizadeh M., Fathi Najafi M., Mousavi Nasab S.D., Davatgar A.M.H., Royaee A., Pilvar P. 2014. Molecular characterization and Phylogenetic Study of Velogenic Newcastle Disease Virus Isolates in Iran. Virus Genes, 48(2):290-295.

 

 

 

 

 

 

 

 

 

 

 

 

 

19. Sarcheshmei M, Dadras H, Mosleh N, Mehrabanpour M J. 2016. Comparative Evaluation of the Protective Efficacy of Different Vaccination Programs Against a Virulent Field Strain of the Newcastle Disease Virus in Broilers. Brazilian Journal of Poultry Science, 18(1):363-370.


20. Sharma J.M. 1991. Overview of the Avian Immune System. Veterinary Immunology and Immunopathology, 30(2):7-13.


21. Scott T.R. 2004. Our current understanding of humoral immunity of poultry. Poultry Science, 83:574-579.

22.Suarez D.L. 2020.  Diseases of Poultry. 14th ed. WILLY Blackwell, pp: 112-166.

23. Thayer S.G., Bread C.W. 2008. A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. The American Association of Avian Pathologists, 23(3):221-232.


24. Winterfield R.W., Dhillon A.S. 1981. Comparative Immune Response from Vaccinating Chickens with lentogenic Newcastle Disease Virus Strains. Poultry Science, 60(2):1195-1203