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Manuscript ID : ASCIJ-2209-1421 (R2) Visit : 213 Page: 231 - 241

10.22034/ascij.2023.1966990.1421

Article Type: Original Research

The Comparison Study of TNF-α Level in Serum and Brain Tissue in Wistar Alzheimer's Rats Treated with Probiotic Lactobacillus and Bifidobacter Strains

Subject Areas : Journal of Animal Biology

Bahareh Jamalzadeh Posht Mesari 1 , Maryam Ghobeh 2 , Parichehr Yaghmaee 3 , Hanieh Jafary 4

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2022-08-03 Accepted : 2022-09-23 Published : 2023-05-22

Keywords: probiotics, Alzheimer's Disease, Amyloid Plaques, Amyloid beta, Tumor Necrosis Factor-alpha (TNF-α),

Abstract :

Alzheimer's disease is a type of brain dysfunction that gradually weakens the patient's mental abilities. Numerous studies have shown that inflammatory mechanisms also play a key role in the pathogenesis of Alzheimer's disease. In this regard, targeting TNF-α using its inhibitors can be an effective therapeutic strategy in controlling and treating such diseases. The purpose of this study was a comparison study of TNF-α levels in blood serum and brain tissue in Wistar Alzheimer ҆s rats treated with probiotic Lactobacillus and Bifidobacter strains by ELISA. The number of amyloid plaques was also counted. For this purpose, 30 adults male Wistar rats were randomly distributed to 5 groups as follows (n = 6): The control group included animals receiving normal water; the Alzheimer's group who underwent surgery and became Alzheimer 's-induced by injection of beta-amyloid; Sham group who underwent surgery and was injected with water (beta-amyloid solvent); two experimental groups who underwent Alzheimer's surgery with beta-amyloid injection and each group received probiotic strains Limosilactobacillus reuteri  and Bifidobacterium longum separately at a dose of 2.5 × 109 CFU at 0.5 CC by gavage for one month. The results demonstrated that both strains, especially Bifidobacterium longum, were able to significantly (p < 0.001) reduce the amount of TNF-α in brain tissue and blood serum compared to the Alzheimer's group. Also, both strains, especially strain Bifidobacterium longum, showed notable capability of inhibiting amyloid plaques. Therefore, it seems that two probiotic strains Limosilactobacillus reuteri and Bifidobacterium longum can be good candidates to reduce the level of TNF-α in both blood serum and brain tissue and also the number of amyloid plaques in Alzheimer's-induced rats. .

References:


  1. Akbari E., Asemi Z., Daneshvar Kakhaki R., Bahmani F., Kouchaki E., Tamtaji O.R., Salami M. 2016. Effect of probiotic supplementation on cognitive function and metabolic status in Alzheimer's disease: a randomized, double-blind and controlled trial. Frontiers in Aging Neuroscience, 8: 256.
    2.
  2. Akkasheh G., Kashani-Poor Z., Tajabadi-Ebrahimi M., Jafari P., Akbari H., Taghizadeh M., Esmaillzadeh A. 2016. Clinical and metabolic response to probiotic administration in patients with major depressive disorder: a randomized, double-blind, placebo-controlled trial. Nutrition, 32(3):315-320.
    3.
  3. Arseneault-Bréard J., Rondeau I., Gilbert K., Girard S.A., Tompkins T.A., Godbout R., Rousseau G. 2012. Combination of Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces post-myocardial infarction depression symptoms and restores intestinal permeability in a rat model. British Journal of Nutrition, 107(12):1793-1799.
    4.
  4. Asl Z.R., Sepehri G., Salami M. 2019. Probiotic treatment improves the impaired spatial cognitive performance and restores synaptic plasticity in an animal model of Alzheimer's disease. Behavioural Brain Research, 376:112183.
    5.
  5. Athari Nik Azm S., Djazayeri A., Safa M., Azami K., Ahmadvand B., Sabbaghziarani F., Vafa M. 2018. Lactobacilli and bifidobacteria ameliorate memory and learning deficits and oxidative stress in β-amyloid (1–42) injected rats. Applied Physiology, Nutrition, and Metabolism, 43(7):718-726.
    6.
  6. Backhed F., Ley R.E., Sonnenburg J.L., Peterson D.A., Gordon J.I. 2005. Host-bacterial mutualism in the human intestine. Science, 307(5717):1915-1920.
    7.
  7. Chelakkot C., Ghim J., Ryu S.H. 2018. Mechanisms regulating intestinal barrier integrity and its pathological implications. Experimental and Molecular Medicine, 50(8):1-9.
    8.
  8. Cheng X., Shen Y., Li R. 2014. Targeting TNF: a therapeutic strategy for Alzheimer's disease. Drug Discovery Today, 19(11): 1822-1827.
    9.
  9. Davari S., Talaei S.A., Alaei H. 2013. Probiotics treatment improves diabetes-induced impairment of synaptic activity and cognitive function: behavioral and electrophysiological proofs for microbiome–gut–brain axis. Neuroscience, 240: 287-296.
    10.
  10. Desbonnet L., Garrett L., Clarke G., Bienenstock J., Dinan T.G. 2008. The probiotic Bifidobacteria infantis: an assessment of potential antidepressant properties in the rat. Journal of Psychiatric Research, 43(2):164-174.
    11.
  11. Desbonnet L., Garrett L., Clarke G., Kiely B., Cryan J.F., Dinan T. 2010. Effects of the probiotic Bifidobacterium infantis in the maternal separation model of depression. Neuroscience, 170(4):1179-1188.
    12.
  12. Farajzadeh D., Karimi-Gharigh S., Jalali-Kondori P., Dastmalchi S. 2019. Design and construction of a novel humanized single-chain variable-fragment antibody against the tumor necrosis factor alpha. Iranian Journal of Pharmaceutical Research, 18(1):308.
    13.
  13. Gareau M.G., Wine E., Rodrigues D.M., Cho J.H., Whary M.T., Philpott D.J., Sherman P. M. 2011. Bacterial infection causes stress-induced memory dysfunction in mice. Gut, 60(3):307-317.
    14.
  14. Hadizadeh M., Hamidi G.A., Salami M. 2019. Probiotic supplementation improves the cognitive function and the anxiety-like behaviors in the stressed rats. Iranian Journal of Basic Medical Sciences, 22(5): 506.
    15.
  15. Han S.H., Park J.C., Mook-Jung I. 2016. Amyloid β-interacting partners in Alzheimer's disease: from accomplices to possible therapeutic targets. Progress in Neurobiology, 137:17-38.
    16.
  16. Kang D.W., Adams J.B., Gregory A.C., Borody T., Chittick L., Fasano A., Krajmalnik-Brown R. 2017. Microbiota transfer therapy alters gut ecosystem and improves gastrointestinal and autism symptoms: an open-label study. Microbiome, 5(1):1-16.
    17.
  17. Katsumoto A., Takeuchi H., Takahashi K., Tanaka F. 2018. Microglia in Alzheimer's disease: risk factors and inflammation. Frontiers in Neurology, 9: 978.
    18.
  18. Kim G.H., Kim J.E., Rhie S.J., Yoon S. 2015. The role of oxidative stress in neurodegenerative diseases. Experimental neurobiology, 24(4):325.
    19.
  19. Kiousi D.E., Karapetsas A., Karolidou K., Panayiotidis M.I., Pappa A., Galanis A. 2019. Probiotics in extraintestinal diseases: current trends and new directions. Nutrients, 11(4):788.
    20.
  20. Koboziev I., Webb C.R., Furr K.L., Grisham M.B. 2014. Role of the enteric microbiota in intestinal homeostasis and inflammation. Free Radical Biology and Medicine, 68:122-133.
    21.
  21. Kuriyama K., Honma M., Koyama S., Kim Y. 2011. D-cycloserine facilitates procedural learning but not declarative learning in healthy humans: a randomized controlled trial of the effect of D-cycloserine and valproic acid on overnight properties in the performance of non-emotional memory tasks. Neurobiology of Learning and Memory, 95(4):505-509.
    22.
  22. Lanctôt K.L., Herrmann N., Mazzotta P., Khan L.R., Ingber N. 2004. GABAergic function in Alzheimer's disease: evidence for dysfunction and potential as a therapeutic target for the treatment of behavioural and psychological symptoms of dementia. The Canadian Journal of Psychiatry, 49(7):439-453.
    23.
  23. Liu W.H., Chuang H.L., Huang Y.T., Wu C.C., Chou G.T., Wang S., Tsai Y.C. 2016. Alteration of behavior and monoamine levels attributable to Lactobacillus plantarum PS128 in germ-free mice. Behavioural Brain Research, 298: 202-209.
    24.
  24. Mallikarjuna N., Praveen K., Yellamma K. 2016. Role of Lactobacillus plantarum MTCC1325 in membrane-bound transport ATPases system in Alzheimer's disease-induced rat brain. BioImpacts, 6(4):203.
    25.
  25. Mehrabadi S., Sadr S.S. 2020. Assessment of probiotics mixture on memory function, inflammation markers, and oxidative stress in an Alzheimer's disease model of rats. Iranian Biomedical Journal, 24(4):220.
    26.
  26. Mohammadi A.A., Jazayeri S., Khosravi-Darani K., Solati Z., Mohammadpour N., Asemi Z., Eghtesadi S. 2016. The effects of probiotics on mental health and hypothalamic–pituitary–adrenal axis: a randomized, double-blind, placebo-controlled trial in petrochemical workers. Nutritional Neuroscience, 19(9): 387-395.
    27.
  27. Parker E.A., Roy T., D'Adamo C.R., Wieland L.S. 2018. Probiotics and gastrointestinal conditions: An overview of evidence from the Cochrane Collaboration. Nutrition, 45:125-134.
    28.
  28. Pitsikas N., Boultadakis A. 2009. Pre-training administration of anesthetic ketamine differentially affects rats' spatial and non-spatial recognition memory. Neuropharmacology, 57(1):1-7.
    29.
  29. Plaza-Díaz J., Ruiz-Ojeda F.J., Vilchez-Padial L.M., Gil A. 2017. Evidence of the anti-inflammatory effects of probiotics and synbiotics in intestinal chronic diseases. Nutrients, 9(6):555.
    30.
  30. Reid C.A., Jackson G.D., Berkovic S. F., Petrou S. 2010. New therapeutic opportunities in epilepsy: a genetic perspective. Pharmacology and Therapeutics, 128(2):274-280.
    31.
  31. Roy C. C., Kien C. L., Bouthillier L., Levy E. 2006. Short‐chain fatty acids: ready for prime time. Nutrition in Clinical Practice, 21(4):351-366.
    32.
  32. Savignac H.M., Tramullas M., Kiely B., Dinan T.G., Cryan J.F. 2015. Bifidobacteria modulate cognitive processes in an anxious mouse strain. Behavioural Brain Research, 287:59-72.
    33.
  33. Schroeder B.O., Bäckhed F. 2016. Signals from the gut microbiota to distant organs in physiology and disease. Nature medicine, 22(10):1079-1089.
    34.
  34. Smith C.J., Emge J.R., Berzins K., Lung L., Khamishon R., Shah P., Gareau M.G. 2014. Probiotics normalize the gut-brain-microbiota axis in immunodeficient mice. American Journal of Physiology-Gastrointestinal and Liver Physiology, 307(8):G793-G802.
    35.
  35. Stefanko D.P., Barrett R. M., Ly A.R., Reolon G.K., Wood M.A. 2009. Modulation of long-term memory for object recognition via HDAC inhibition. Proceedings of the National Academy of Sciences, 106(23):9447-9452.
    36.
  36. Takemiya T., Suzuki K., Sugiura H., Yasuda S., Yamagata K., Kawakami Y., Maru E. 2003. Inducible brain COX-2 facilitates the recurrence of hippocampal seizures in mouse rapid kindling. Prostaglandins and Other Lipid Mediators, 71(3-4):205-216.
    37.
  37. Tapia-Arancibia L., Aliaga E., Silhol M., Arancibia S. 2008. New insights into brain BDNF function in normal aging and آلزایمر disease. Brain Research Reviews, 59(1): 201-220.
    38.
  38. Tillisch K., Labus J., Kilpatrick L., Jiang Z., Stains J., Ebrat B., Mayer E.A. 2013. Consumption of fermented milk product with probiotic modulates brain activity. Gastroenterology, 144(7):1394-1401.
    39.
  39. Wall R., Marques T.M., O’Sullivan O., Ross R.P., Shanahan F., Quigley E.M., Stanton C. 2012. Contrasting effects of Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 on the composition of murine brain fatty acids and gut microbiota. The American Journal of Clinical Nutrition, 95(5):1278-1287.
    40.
  40. Westfall S., Lomis N., Kahouli I., Dia S.Y., Singh S.P., Prakash S. 2017. Microbiome, probiotics and neurodegenerative diseases: deciphering the gut brain axis. Cellular and Molecular Life Sciences, 74(20):3769-3787.
    41.
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