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Manuscript ID : ASCIJ-2208-1419 Visit : 278 Page: 205 - 215

10.22034/ascij.2022.1966260.1419

Article Type: Original Research

The Effect of a Period of High-intensity Interval Training on the Expression of Plasma Levels of AST and ALT Enzymes in the Cardiac Tissue of Wistar Rats with Fatty Liver

Subject Areas : Journal of Animal Biology

Elham Mirzaeyan 1 , Mania Roozbayani 2 , Hossein Shirvani 3

1 - Department of ExercisePhysiology, Boroujerd, Branch, Islamic Azad University, Boroujerd, Iran
2 - Department of ExercisePhysiology, Boroujerd, Branch, Islamic Azad University, Boroujerd, Iran
3 - Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Received: 2022-08-25 Accepted : 2022-10-23 Published : 2023-05-22

Keywords: Fatty liver, AST, ALT Enzyme, HIIT Exercise,

Abstract :

High-intensity interval training (HIIT) is a kind of exercise training that is suggested for individuals with sufficient time for regular training.  This study aimed to evaluate the effect of a period of intense intermittent training on the expression of plasma levels of AST, and ALT enzyme in the cardiac tissue of male rats with fatty liver (steatosis model). The statistical sample consisted of 48 male Wistar rats (in2 healthy models with fatty liver) aged 5 weeks and weighing 200-250 and in the same conditions (12:12 darkness and light, temperature 22 ± 2 degrees Celsius (50-60%) humidity (51% humidity) were kept in cages. Gene expression was assessed using Real-timePCR and AST, and ALT enzyme was assessed using ZiestChem diagnostic kits. Data were analyzed by independent t-test, one-way analysis of variance, and Tukey's post hoc test, and the data were analyzed by SPSS software version 22. According to the results, the expression of ASTandALT enzymes in the rats of the patient group increased compared to the rats of the healthy group (p < 0.05), which according to the level of significance It was significant. It seems that a period of intense intermittent training affects the expression of plasma levels of ASTand ALT enzymes in the cardiac tissue of male rats with fatty liver (steatosis model) and can serve as a marker for the prevention and treatment of diseases. Use cardiovascular, fatty liver, obesity as well as diabetes. Keywords:, AST, ALT anzem, HIIT exercise

References:


  1. Al-Jarrah M.D., Jamous M., 2011. Effect of endurance exercise training on the expression of GFAP, S100B and NSE in the striatum of chronic/progressive mouse model of Parkinson's disease. NeuroRehabilitation, 28(4): 359.
    2.
  2. Aoyagi T., Ishikawa Y., Oshikawa H., Kinugawa K., Yokoyama I., Nagai R. Caveolin-3 is up-regulated in the physiological left ventricular hypertrophy induced by voluntary exercise taining in rats. Journal of Sports Science and Medicine, 1:141-146.
    3.
  3. Boluyt M.O., Brevick J.L., Rogers D.S., Randall M.J., Scalia A.F., Li Z.B. Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20. Proteomics, 6(10):3154-69.
    4.
  4. Burgomaster K.A., Howarth K.R., Phillips S.M., Rakobowchuk M, Macdonald M.J. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. Journal of Physiology, 586:151-160.
    5.
  5. Chen Z., Hu Q., Xie Q., Wu S., Pang Q., Liu M., Zhao Y., Tu F., Liu C., Chen X. Effects of treadmill exercise on motor and cognitive function recovery of MCAO mice through the caveolin-1/VEGF signaling pathway in ischemic penumbra. Neurochemical Research, 44(4):930-946.
    6.
  6. Chen Y., Schubert M., Palumbo E., Striling D. 2019. Therapy inhibits multiple inflammatory pathways and ameliorates autoimmune encephalomyelitis. The Journal of Clinical Investigation, 44(4): 1-8.
    7.
  7. Cho J., Koh Y., Han J., Kim D., Kim T., Kang H. Adiponectin mediates the additive effects of combining daily exercise with caloric restriction for treatment of non-alcoholic fatty liver. International Journal of Obesity, 40(11):1760-1767.
    8.
  8. Chosa E., Sekimoto T., Sonoda N., Yamamoto K., Matsuda H., Takahama K., Tajima N. Evaluation of human β- enolase as a serum marker for exercise-induced muscle damage. Clinical Journal of Sport Medicine, 13(4):209-212.
    9.
  9. Chosa E., Sekimoto T, Sonoda N, Yamamoto K, Matsuda H, Takahama K, Tajima N. Evaluation of human β-enolase as a serum marker for exercise-induced muscle damage. Clinical Journal of Sport Medicine, 13(4):209-212.
    10.
  10. Das M., Das D.K., Matsuda M., Nishizawa M., Segawa K., Tanaka M. Caveolin and MAP kinase interaction in angiotensin II preconditioning of the myocardium. Journal of cellular and Molecular Medicine, 11(4):788-797.
    11.
  11. Davoodi M., Moosavi H., Nikbakht M. 2012. The effect of eight weeks selected aerobic exercise on liver parenchyma and liver enzymes (AST, ALT) of fat liver patients. Journal of Shahrekord Uuniversity of Medical Sciences, 14(1):84-90.
    12.
  12. Fowler W., Gardner G.W., Kazerunian H.H., Lauvstad W.A. The effect of exercise on serum enzymes. Arch Phys Med Rehabil 1968. Bloor C Effects of exercise on enzyme interpretation. Archives of Physical Medicine and Rehabilitation, 49(10):554-565.
    13.
  13. Freimann S., Kessler-Icekson G., Shahar I., Radom-Aizik S., Yitzhaky A., Eldar M., Scheinowitz M. Exercise training alters the molecular response to myocardial infarction. Medicine Science and Sports Exercise, 41(4):757-765.
    14.
  14. Hallsworth, Fattakhova G., Hollingsworth K.G., Thoma C., Moore S., Taylor R., Day C.P., Trenell M.I. 2011. Resistance exercise reduces liver fat and its mediators in non-alcoholic fatty liver disease independent of weight loss. Gut, 60(9): 1278-1283.
    15.
  15. Hallsworth K., Loennechen J.P., Dunn S.L. 2016. Resistance exercise reduces liver fat and its mediators in non-alcoholic fatty liver disease independent of weight loss. Gut, 4(2): 61-67.
    16.
  16. Illera J, Haram PM, Tjønna AE, Helgerud J, Slørdahl SA. Photoperiod variations of various blood biochemistry constants in the rabbit. Revista Espanola de fisiologia. 48(1):1-7
    17.
  17. Ishikawa Y., Oshikawa H., Kinugawa K., Yokoyama I., Nagai R. 2002. Caveolin-3 is Up-Regulated in the Physiological Left Ventricular Hypertrophy Induced by Voluntary Exercise Training in Rats. Journal of Sports Sciencea and Medicine, 1(4):141-6.
    18.
  18. Johnson N.A., Sachinwalla T., Walton D.W., Smith K., Armstrong A., Thompson M.W., George J. Aerobic. exercise training reduces hepatic and visceral lipids in obese individuals without weight loss. Hepatology, 50(4):1105-1112.
    19.
  19. Khanmohammadi R., Azarbaijani M., Piri M., Khorsandi L. The effect of severe periodic training and crocin on oxidative stress in male rats subjected to doxorubicin induction. Armaghane Danesh, 23(6):694-708.
    20.
  20. Leandro C.G., Levada A.C., Hirabara S.M., Manhães-de-Castro R., De-Castro C.B., Curi R., Pithon-Curi T.C. A program of moderate physical training for Wistar rats based on maximal oxygen consumption. Journal of Strength and Conditioning Research, 21(3):751.
    21.
  21. Lee, Y.I., Cho J.Y., Kim M.H., Kim K.B., Lee D.J., Lee K.S. Effects of exercise training on pathological cardiac hypertrophy related gene expression and apoptosis. European Journal of Applied Physiology, 97(2):216-224.
    22.
  22. Musumeci O., Wisloff U., Coombes J.S. 2014. Recurrent rhabdomyolysis due to muscle β-enolase deficiency very rare or underestimated? Journal of Neurology, 261(12):2424-2428.
    23.
  23. Nytrøen K., Rustad L.A., Erikstad I., Aukrust P., Ueland T., Lekva T., Gude E., Wilhelmsen N., Hervold A., Aakhus S., Gullestad L., Arora S. 2017. The effect of high intensity interval training on cardiac allograft v The Journal of Heart and Lung Transplantation, 32(11): 1073-1080.
    24.
  24. Orci L., Mizushima K., Gobelet C., Little JP. Exercise based interventions for nonalcoholic fatty liver disease: a meta-analysis and meta-regression. Clinical Gastroenterology and Hepatology, 14(10): 1398-1411.
    25.
  25. Park S., Dalleck L.C.,  Tjonna  A.E.,  Beetham K.S., Coombes J.S.  Differential expression of caveolins and myosin heavy chains in response to forced exercise in rats. Laboratory Animal Research, 28(1):1-9.
    26.
  26. Patel, H.H., Head B.P., Petersen H.N., Niesman I.R, Huang D., Gross G.J., Insel P.A., Roth D.M. Protection of adult rat cardiac myocytes from ischemic cell death: role of caveolar microdomains and delta-opioid receptors. American Journal of Physiology: Heart and Circulatoty, Physiology, 291(1):H344-350.
    27.
  27. PodhorskaOkołów, Yoshikawa T., Hood M.S., Little J.P., Tarnopolsky M.A. 2006. Expression of metallothionein in renal tubules of rats exposed to acute and endurance exercise. Folia Histochemica et Cytobiologica, 44(3):195-200.
    28.
  28. Rahmani F., Tanaka S., Saito K., Shu M., Sone Y., Hood M.S. 2008. Acute effects of aerobic and resistance exercises on serum leptin and risk factors for coronary heart disease in obese females. Sport Sciences for Health, 2(3):108-117.
    29.
  29. Ramos J.S., Dalleck L.C., Tjonna A.E., Beetham K.S., Coombes J.S. 2015. The impact of high-intensity interval training versus moderateinten-sity continuous training on vascular function: a systematic review and meta-analysis. Sports and Medicine, 45(5):679-692.
    30.
  30. Roh H.T., So W.Y. 2017. The effects of aerobic exercise training on oxidant–antioxidant balance, neurotrophic factor levels, and blood–brain barrier function in obese and non-obese men. Journal of Sport and Health Science, 6(4):447-453.
    31.
  31. Scalco, R.S., Gardiner A.R., Pitceathly R.D., Hilton-Jones D., Schapira A.H., Turner C., Parton M., Desikan M., Barresi R., Marsh J., Manzur A.Y., Childs A.M., Feng L., Murphy E., Lamont P.J., Ravenscroft G., Wallefeld W., Davis M.R., Laing N.G., Holton J.L., Fialho D., Bushby K., Hanna M.G., Phadke R., Jungbluth H., Houlden H., Quinlivan R. CAV3 mutations causing exercise intolerance, myalgia and rhabdomyolysis: Expanding the phenotypic spectrum of caveolinopathies. Neuromuscular Disorders, 26(8):504-510.
    32.
  32. Shamsoddini A., Lienkens S., Ichikawa H., Connon C.E., Stensel D.J. Effect of aerobic and resistance exercise training on liver enzymes and hepatic fat in Iranian men with nonalcoholic fatty liver disease. Hepatitis Monthly, 15(10): e31434.
    33.
  33. Tartibain B., Sharifi , Ebrahemi T.B., Astorino T.A., Schubert M.M., Palumbo E. 2016. The effect of 12–week high-intensity Interval training (HIIT)on lung function, serum leptin level and lipid profiles in inactive obese men. 11(5): e0153976.
    34.
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