Evaluation of Antioxidant, Anti-inflammatory and Antibacterial P with Anbernesara Smoke Extract
Subject Areas :
Journal of Animal Biology
Roya Mahdizadeh
1
,
Mohsen Firoozrai
2
,
Masoud Homayouni Tabrizi
3
,
Seyed Javad Hoseini
4
1 - Department of chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
2 - Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
3 - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4 - Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Received: 2023-02-08
Accepted : 2023-04-29
Published : 2023-11-22
Keywords:
Antibacterial,
Anti-inflammatory,
Anti-oxidant,
PLGA nanoparticles,
Organic extract of Anbernesara smoke,
Abstract :
The aim of this study is to synthesize and investigate the anti-oxidant, anti-inflammatory and anti-bacterial effects of polylactic acid (PLGA) nanoparticles containing the extract of Anbernesara smoke. For this purpose, Anbernesara smoke was extracted and encapsulated by PLGA nanoparticles using the W1/O/W2 method and characterized by DLS, zeta potential, FESEM and FTIR electron microscope techniques. The antioxidant activity of the synthesized nanoparticles using ABTS and FRAP tests, as well as measuring the expression levels of the antioxidant genes catalase (CAT) and superoxide dismutase (SOD) and the expression of interleukin 6 (IL-6) and interleukin 10 (IL- 10) was also analyzed to determine the anti-inflammatory potential of nanoparticles by Real time PCR test. The antibacterial activity of the synthesized nanoparticles was evaluated on two bacterial strains Escherichia coli and Bacillus subtilis. The results showed the formation of spherical nanoparticles with an average size of 51.2 nm, dispersion index of 0.22, surface’s charge of -35.37 mV and encapsulation efficiency of 74%. The antioxidant properties of synthesized PLGA nanoparticles were confirmed by reducing Fe+3, inhibiting the formation of free radicals and increasing the expression level of CAT and SOD genes. The expression levels of IL-6 and IL-10 genes were also increased under the influence of PLGA nanoparticles loaded with the organic extract of Anbernesara smoke, indicating the anti-inflammatory properties of the proposed nanoparticles. PLGA nanoparticles loaded with the organic extract of Anbernesara smoke had an antibacterial effect on B. subtilis, but not on E. coli. The PLGA nanoparticles loaded with the organic extract of Anbernesara smoke have anti-oxidant, anti-inflammatory properties and can be considered as a combination with antibacterial potential.
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References:
Alhajamee M., Marai K., Al Abbas S.M.N. Homayouni Tabrizi M. 2021. Co-encapsulation of curcumin and tamoxifen in lipid-chitosan hybrid nanoparticles for cancer therapy. Materials Technology, 37(18):1-12.
Ataei Moghadam S., Rostami Charati F., Akbari R., Gholamalipour Alamdari E. Behmanesh B. 2020. Consideration antimicrobial and antioxidant properties of anbarnesa smoke ointment. Journal of Medicinal and Chemical Sciences, 3(3):245-253.
Chen H., Lui Y.S., Zhao J., Xu L. Tan L.P. 2018. Effect of solvent composition of electrospun PLGA fibers on paclitaxel release. Materials Technology, 33(11):716-722.
Chiu H.I., Samad N.A., Fang L., Lim V. 2021. Cytotoxicity of targeted PLGA nanoparticles: a systematic review. RSC Advances, 11(16):9433-9449.
Foroutan Koudehi M., Imani Fooladi A.A., Aghozbeni E.A.H., Nourani M.R. 2019. Nano bioglass/gelatin scaffold enhanced by nanosilver as an antibacterial conduit for peripheral nerve regeneration. Materials Technology, 34(13):776-784.
Joharchi K., Anaraki Firouz S.M., Mashhadiabbas F., Mansouri A., Shafiee H., Taheri J.B. 2020. Wound healing and the effect of ANNAS; a new product of AnbarNesa. Jundishapur Journal of Natural Pharmaceutical Products, 15(2):e66668.
Khshemat V., Homayouni-Tabrizi M., Neamati A., Khadem F., Irani M. 2021. Fabrication, characterisation, and biological properties of chitosan nanoparticles containing rapeseed pollen extract (RPE) on the MCF-7 cell line. Materials Technology, 37(14):1-11.
Li Y., Liu L., Qu X., Ren L., Dai K. 2015. Drug delivery property, antibacterial performance and cytocompatibility of gentamicin loaded poly (lactic-co-glycolic acid) coating on porous magnesium scaffold. Materials Technology, 30(sup6):B96-B103.
Ma Z., Fan Y., Wu Y., Kebebe D., Zhang B., Lu P., Pi J., Liu Z. 2019. Traditional Chinese medicine-combination therapies utilizing nanotechnology-based targeted delivery systems: a new strategy for antitumor treatment. International Journal of Nanomedicine, 14:2029.
Mohammadi G., Valizadeh H., Barzegar-Jalali M., Lotfipour F., Adibkia K., Milani M., Azhdarzadeh M., Kiafar F., Nokhodchi A. 2010. Development of azithromycin–PLGA nanoparticles: Physicochemical characterization and antibacterial effect against Salmonella typhi. Colloids and Surfaces B: Biointerfaces, 80(1):34-39.
Moral R., Moreno-Caselles J., Perez-Murcia M., Perez-Espinosa A., Rufete B., Paredes C. 2005. Characterisation of the organic matter pool in manures. Bioresource Technology, 96(2):153-158.
Mousavi-Khattat M., Nourbakhshan H., Roumi M., Ebrahiminejad M., Fazeli Y., Shakeran Z., 2021. Anticancer activity of silver nanoparticles synthesized using extract of animal waste as a traditional medicine on MCF-7 human breast cancer cell line. SSRN Electronic Journal, 10.21203/rs.3.rs-412407/v1.
Muhamad N., Plengsuriyakarn T., Na-Bangchang K. 2018. Application of active targeting nanoparticle delivery system for chemotherapeutic drugs and traditional/herbal medicines in cancer therapy: a systematic review. International Journal of Nanomedicine, 13:3921.
Mukherjee A., Bhattacharyya S. 2020. Nanotechnology in medicine. Biotechnology Business-Concept to Delivery, 2020:57-64.
Palmqvist E., Hahn-Hägerdal B. 2000. Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresource Technology, 74(1):25-33.
Pandey P., Rahman M., Bhatt P.C., Beg S., Paul B., Hafeez A., Al-Abbasi F.A., Nadeem M.S., Baothman O., Anwar F. 2018. Implication of nano-antioxidant therapy for treatment of hepatocellular carcinoma using PLGA nanoparticles of rutin. Nanomedicine, 13(8):849-870.
Parvin N., Validi M., Banitalebi M., Mobini G.R., Ashrafi K., Farrokhi E., Rafieian-Kopaei M., Akbari N., Safdari Dehcheshmeh F., Rafiee Vardanjani L. 2010. Effect of medicinal smokes on some nosocomial infection factors. Journal of Shahrekord Uuniversity of Medical Sciences, 12(2):76-83.
Patel J.B., Cockerill F., Bradford P.A. 2015. Performance standards for antimicrobial susceptibility testing: twenty-fifth informational supplement. Clinical and Laboratory Standards Institute, 35(3):240.
Rajendran A., Vinoth G., Shanthi V., Barik R., Pattanayak D., 2014. Silver nano particle incorporated Ti metal prepared by chemical treatment for antibacterial and corrosion resistance study. Materials Technology, 29(sup1):B26-B34.
Rajurkar N.S., Hande S. 2011. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian Journal of Pharmaceutical Sciences, 73(2):146.
Rezvantalab S., Moraveji M.K. 2019. Microfluidic assisted synthesis of PLGA drug delivery systems. RSC Advances, 9(4):2055-2072.
Rinita J., Jose R., Jothi N.N. 2020. Acclimated growth and characterization of manganese oxide nanospheres using natural extract from trigonella foenum-graecum. Materials Technology, 36(5):1-8.
Saxena S.K., Nyodu R., Kumar S., Maurya V.K. 2020. Current advances in nanotechnology and medicine. NanoBioMedicine, 2020: 3-16.
Schoubben A., Ricci M., Giovagnoli S. 2019. Meeting the unmet: From traditional to cutting-edge techniques for poly lactide and poly lactide-co-glycolide microparticle manufacturing. Journal of Pharmaceutical Investigation, 49(4):381-404.
Shabestarian H., Homayouni Tabrizi M., Movahedi M., Neamati A., Sharifnia F. 2021. Putative mechanism for cancer suppression by PLGA nanoparticles loaded with Peganum harmala smoke extract. Journal of Microencapsulation, 38(5):324-337.
Shafiee H., Mortazavi H., Baharv M., Eslami G., Bakhtiari S., Taheri S., Namazi F., Asgari A., Azimi S., Joharchi K. 2012. Iranian traditional medicine: Comparison of the antibacterial effect of ANNAS 0.2% and chlorhexidine 0.2%. African Journal of Microbiology Research, 6(15):3600-3603.
Shafiee H.A., Moravej-Salehi E. 2015. Anbarnesa: The Past Tradition, the Future Medicine. Iranian Red Crescent Medical Journal, 17(12): e29536.
Shafiee H.A., Motamedi M.H.K., Mina M., Taheri J.B., Azimi S., Joharchi K., Yadegari Z., Rasouli H.R. 2014. Evaluation of cytotoxic effects of Anbarnesa on fibroblast L929: Can it be used as a mouthwash? Ancient Science of Life, 33(4):203.
Stagos D. 2020. Antioxidant activity of polyphenolic plant extracts, Multidisciplinary Digital Publishing Institute.
Swider E., Koshkina O., Tel J., Cruz L.J., de Vries I.J.M., Srinivas M. 2018. Customizing poly (lactic-co-glycolic acid) particles for biomedical applications. Acta Biomaterialia, 73:38-51.
Taheri J.B., Azimi S., Rafieian N., Zanjani H.A. 2011. Herbs in dentistry. International Dental Journal, 61(6):287-296.
Talebi A., Harigh E., Dehdashtian E., Amini F., Meshkat M., 2017. Evaluation of the effect of smoke of Anbar Nasara (Donkey dung) extract on staphilococcus aureus and Bacillus subtilis.
Thein-Han W., Misra R. 2009. Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomaterialia, 5(4):1182-1197.
Tonekaboni H. 2007. The Present for the Faithful (Tohfat ol momenin). Tehran: Research Center of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Nashre Shahr Press.
Wu P., Zhou Q., Zhu H., Zhuang Y., Bao J. 2020. Enhanced antitumor efficacy in colon cancer using EGF functionalized PLGA nanoparticles loaded with 5-Fluorouracil and perfluorocarbon. BMC Cancer, 20:1-10.
Yashin A., Yashin Y., Xia X., Nemzer B. 2017. Antioxidant activity of spices and their impact on human health: A review. Antioxidants, 6(3):70.
Zhang R., Liu F., Tian Y., Cao W., Wang R. 2021. Nanotechnology in Traditional Medicines and Natural Products. Frontiers in Chemistry, 9: doi.org/10.3389/fchem.2021. 633419.
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