Evaluation of the Effect of Ferula gummosa Extract on Biochemical Parameters of a Skin Wound in NMRI Mice

Subject Areas : Journal of Animal Biology

Parisa Seifali ¹ (Department of Cognitive Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran)
Parichehreh Yaghmaei ² (Department of Cognitive Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran)
Nasim Hayati Roodbari ³ (Department of Cognitive Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran)

Received: 2022-08-13 Accepted : 2022-09-07 Published : 2023-05-22

Keywords: Oxidative stress, Skin wound, Ferula gummosa, Eucerin,

Abstract :

Wound healing is one of the important problems in medicine. Therefore, this study aimed to investigate the effect of Ferula gummosa extract on skin wound healing in NMRI mice. 24 NMRI mice with skin ulcers, were treated with Ferula gummosa ointment on days 3, 6,12, and 21, and randomly divided into three eucerin groups (daily recipient eucerin ointment), the experimental group1(daily treatment with Ferula gummosa ointment at a dose of 2 mg) and the experimental group 2 (daily treatment with Ferula gummosa ointment at a dose of 4 mg and the ulcers were measured with a calliper and the biochemical indexes of superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity (TAC) in tissue, were measured. Then, tissue sections were prepared from the wound and evaluated histopathologically by hematoxylin-eosin staining. The data were analyzed by SPSS 22 software and the Tukey test at a significance level of p < 0.05 and the histograms were drawn in excel. wound size in the Ferula gummosa groups compared to the Eucerin group showed a significant decrease (p < 0.05). The concentration of MDA in groups the Ferula gummosa significantly decreased time-dependent compared with the eucerin group (p < 0.05). SOD activity and TAC concentration also showed a significant increase in time-dependent in groups Ferula gummosa compared to the eucerin group (p < 0.05). Based on histopathological findings, epidermal and dermal thickness increased significantly in groups Barijeh compared to eucerin group (p < 0.05), and the number of fibroblasts and blood vessels in the two groups of Barijeh in a time-dependent manner compared to the Eucerin group showed a significant increase (p < 0.05). According to the results, it was shown that Ferula gummosa is effective in wound healing and repair by increasing SOD and TAC activity and decreasing MDA concentration, as well as histopathological results. Therefore, the findings of this study showed a new perspective on this herbal medicine for us wound healing. .

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