Design, Synthesis, Characterization and Bioactivity Evaluation of Chitosan-Grafted Alumina Nanoparticles
Subject Areas :
Journal of Animal Biology
Elham Rostami
1
,
Elham Hovazi
2
1 - Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, 67149, Ahvaz, Iran
2 - Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, 67149, Ahvaz, Iran
Received: 2021-04-14
Accepted : 2021-07-25
Published : 2022-02-20
Keywords:
Toxicity,
Chitosan,
Biodegradable,
Alumina nanoparticle,
Abstract :
Nowadays, the nanotechnology development has expanded to various medical fields, drug delivery, etc. novel drug delivery systems have made diseases cure such as cancer easier. Alumina nanoparticles have a vast use in this area due to their thermal properties. Among numerous applications uses of these nanoparticles, one of the hazards to discuss is their toxicity. Besides, surface modification of these nanoparticles with chitosan as a biopolymer lead to the bioavailability improvement. In this research, alumina NPs were covered with chitosan biopolymer. Analysis of the size of particles indicated about 70 nm. FT-IR analysis on alumina and alumina/chitosan NPs showed surface modification of alumina NPs greatly. Besides, TEM images of the nanoparticles approved suitable size for drug delivery purposes. HeLa cells were treated with Alumina and Alumina/CS for 24 h, and IC50 concentration was determined. Toxic effects of these components on the cells were evaluated by MTT assay and acridine orange/ethidium bromide (AO/EB) staining. The results of the bioassay of nanoparticles well showed their successful coating by chitosan polymer. Moreover, the results detected that Alumina samples had toxic effects on the cells in a dose dependent manner and their IC50 concentration was about 25 mM. While coated samples had significantly lower toxicity and it had no significant toxicity on HeLa cells in concentrations below 5 mM.
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