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  • Comparative Effect of Timolol and Methanolic Extract of Zataria multiflora with Vitamin E on APO E Gene Expression and Behavioral and Biochemical Changes in Alzheimer's Rats

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Manuscript ID : ASCIJ-2012-1221 (R2) Visit : 270 Page: 23 - 33

10.22034/ascij.2023.1917661.1221

Article Type: Original Research

Comparative Effect of Timolol and Methanolic Extract of Zataria multiflora with Vitamin E on APO E Gene Expression and Behavioral and Biochemical Changes in Alzheimer's Rats

Subject Areas : Journal of Animal Biology

Samira Homayoun Pour 1 , Maryam Bananej 2 , Maryam Khosravi 3 , Hengameh Alibeik 4

1 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2022-02-13 Accepted : 2022-05-16 Published : 2023-05-22

Keywords: vitamin E, methanolic extract, Zataria multiflora, Alzheimer's Disease, Thymolol,

Abstract :

Alzheimer's Disease is the most common form of dementia, characterized by memory and cognitive deficits. Vitamin E, as an antioxidant, plays an important role in reviving free radicals and converting them into safe substances by giving hydrogen. Shirazi thyme (Zataria multiflora) has antibacterial, antifungal and antioxidant properties. The aim of this study is the comparative effect of timolol with methanol extract of Zataria multiflora with vitamin E on Alzheimer's disease in male Wistar rats. 40 male Wistar rats weighing approximately 200 grams were randomly divided into 5 groups of eight. Beta-amyloid 42 was used to induce Alzheimer's disease. Then, methanolic and timolol extracts were extracted from Zataria multiflora. The level of Apo E gene expression was evaluated by Real Time PCR and TC, LDL and HD biochemical factors. The expression of Apo E gene in the experimental groups increased significantly compared to the Alzheimer's group. The expression of this gene was decreased in the sham group compared to the control group. The group of Alzheimer's mice had a significant increase in total cholesterol (TC) and low-density lipoprotein (LDL) serum levels, and the level of HDL showed a significant decrease compared to the control group. Timolol, m ethanolic extract of Zataria multiflora and vitamin E have positive effects on the expression of APOA E gene in leukocytes. On the other hand, with a significant effect on HDL, the methanolic extract of Zataria multiflora can be used as an effective product to prevent and reduce the complications of Alzheimer's disease.  

References:


  1. Aliashrafi S., Ebrahimi-Mameghani M., Jafarabadi M.A., Lotfi-Dizaji L., Vaghef-Mehrabany E., Arefhosseini S.R. 2020. Effect of high-dose vitamin D supplementation in combination with weight loss diet on glucose homeostasis, insulin resistance, and matrix metalloproteinases in obese subjects with vitamin D deficiency: a double-blind, placebo-controlled, randomized clinical trial. Applied Physiology, Nutrition, and Metabolism, 45(10):1092-1098.
    2.
  2. Ang L.S., Cruz R., Hendel A., Granville D.J. 2008. Apolipoprotein E, an important player in longevity and age-related diseases. Experimental Gerontology, 43(7):615-622.
    3.
  3. Ba M., Kong M., Li X., Ng K.P., Rosa-Neto P., Gauthier S. 2016. Is ApoE ɛ 4 a good biomarker for amyloid pathology in late onset Alzheimer’s disease? Translational Neurodegeneration, 5(1):1-4.
    4.
  4. Babri S., Mohaddes G., Feizi I., Mohammadnia A., Niapour A., Alihemmati A., Amani M. 2014. Effect of troxerutin on synaptic plasticity of hippocampal dentate gyrus neurons in a β-amyloid model of Alzheimer׳ s disease: An electrophysiological study. European Journal of Pharmacology, 732:19-25.
    5.
  5. Bagyinszky E., Youn Y.C., An S.S.A., Kim S. 2014. The genetics of Alzheimer’s disease. Clinical interventions in aging, 9:535-551.
    6.
  6. Boyd M., Tennant S., Melendez J., Toema D., Galen J., Geddes C., Levine M. 2015. Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of S almonella detection in blood. Journal of Applied Microbiology, 118(5):1199-1209.
    7.
  7. Calkins M.J., Reddy P.H. 2011. Amyloid beta impairs mitochondrial anterograde transport and degenerates synapses in Alzheimer's disease neurons. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1812(4):507-513.
    8.
  8. Chang T.-Y., Chang 2017. ApoE and lipid homeostasis in Alzheimer’s disease: introduction to the thematic review series. Journal of Lipid Research, 58(5):823-823.
    9.
  9. De Sanctis V., Soliman A.T., Elsedfy H., Soliman N.A., Elalaily R., El Kholy M. 2016. Dysmenorrhea in adolescents and young adults: a review in different countries. Acta Biomedica, 87(3):233-246.
    10.
  10. Deane R., Bell R., Sagare A., Zlokovic B. 2009. Clearance of amyloid-β peptide across the blood-brain barrier: implication for therapies in Alzheimer's disease. CNS and Neurological Disorders-Drug Targets, 8(1):16-30.
    11.
  11. Di Battista M.A., M Heinsinger N., William Rebeck G. 2016. Alzheimer’s disease genetic risk factor APOE-ε4 also affects normal brain function. Current Alzheimer Research, 13(11): 1200-1207.
    12.
  12. Farbood Y., Sarkaki A., Dianat M., Khodadadi A., Haddad M.K., Mashhadizadeh S. 2015. Ellagic acid prevents cognitive and hippocampal long-term potentiation deficits and brain inflammation in rat with traumatic brain injury. Life Sciences, 124:120-127.
    13.
  13. Goryacheva A.V., Kruglov S.V., Pshennikova M.G., Smirin B.V., Malyshev I.Y., Barskov I.V., Viktorov I.V., Downey H.F., Manukhina E.B. 2010. Adaptation to intermittent hypoxia restricts nitric oxide overproduction and prevents beta-amyloid toxicity in rat brain. Nitric Oxide, 23(4):289-299.
    14.
  14. Goudarzi m., Satari M., Najar P.S., Goudarzi G.R., Bigdeli M. 2006. Antibacterial effects of aqueous and alcoholic extracts of Thyme on enterohemorrhagic Escherichia coli. Yafteh, 3(29):63-69.
    15.
  15. Hajipour S., Sarkaki A., Farbood Y., Eidi A., Mortazavi P., Valizadeh Z. 2016. Effect of gallic acid on dementia type of Alzheimer disease in rats: electrophysiological and histological studies. Basic and Clinical Neuroscience, 7(2):97-106.
    16.
  16. Halberstein R.A. 2005. Medicinal plants: historical and cross-cultural usage patterns. Annals of Epidemiology, 15(9):686-699.
    17.
  17. Hamidinia M., Boroujerdnia M.G., Talaiezadeh A., Solgi G., Roshani R., Iranprast S., Khodadadi A. 2015. Increased P-35, EBI3 transcripts and other treg markers in peripheral blood mononuclear cells of breast cancer patients with different clinical stages. Advanced Pharmaceutical Bulletin, 5(2):261-267.
    18.
  18. Hathcock J. 2004. Vitamin and mineral safety. Council for Responsible Nutrition. Washington: CRN Press.
    19.
  19. Huynh T.-P.V., Davis A.A., Ulrich J.D., Holtzman D.M. 2017. Apolipoprotein E and Alzheimer’s disease: the influence of apolipoprotein E on amyloid-β and other amyloidogenic proteins. Journal of Lipid Research, 58(5):824-836.
    20.
  20. Ibrahim S.A., Sabahelkhier M.K. 2017. Evaluation of the toxic influence of vitamin E (dl-alpha-tocopheryl acetate) and treatment with aqueous extracts of cinnamon or anise on lipid profile and liver functions of female wistar rats. International Journal of Medicine, 5(2):248-253.
    21.
  21. Janel N., Alexopoulos P., Badel A., Lamari F., Camproux A., Lagarde J., Simon S., Feraudet-Tarisse C., Lamourette P., Arbones M. 2017. Combined assessment of DYRK1A, BDNF and homocysteine levels as diagnostic marker for Alzheimer’s disease. Translational Psychiatry, 7(6):e1154-e1154.
    22.
  22. Khanahmadi M., Farhud D.D., Malmir M. 2015. Genetic of Alzheimer’s disease: A narrative review article. Iranian Journal of Public Health, 44(7):892-901.
    23.
  23. Khan I.A., Abourashed E.A. 2011. Leung's encyclopedia of common natural ingredients: used in food, drugs and cosmetics: John Wiley and Sons.
    24.
  24. Kim S.R., Choi S.H., Ha C.K., Park S.G., Pyun H.W., Yoon D.H. 2009. Plasma total homocysteine levels are not associated with medial temporal lobe atrophy, but with white matter changes in Alzheimer's disease. Journal of Clinical Neurology, 5(2):85-90.
    25.
  25. Korani M.S., Farbood Y., Sarkaki A., Moghaddam H.F., Mansouri M.T. 2014. Protective effects of gallic acid against chronic cerebral hypoperfusion-induced cognitive deficit and brain oxidative damage in rats. European Journal of Pharmacology, 733:62-67.
    26.
  26. Leal N.S., Schreiner B., Pinho C.M., Filadi R., Wiehager B., Karlström H., Pizzo P., Ankarcrona M. 2016. Mitofusin‐2 knockdown increases ER–mitochondria contact and decreases amyloid β‐peptide production. Journal of Cellular and Molecular Medicine, 20(9):1686-1695.
    27.
  27. Lekar A., Borisenko S., Vetrova E., Sushkova S., Borisenko N. 2014. Extraction of quercetin from Polygonum hydropiper L. by subcritical water. American Journal of Agricultural and Biological Sciences, 9(1):1-5.
    28.
  28. Leo F., Rossodivita A.N., Segni C., Raimondo S., Canichella S., Silvestrini A., Miggiano G.A.D., Meucci E., Mancini A. 2016. Frailty of obese children: evaluation of plasma antioxidant capacity in pediatric obesity. Experimental and Clinical Endocrinology and Diabetes, 124(08):481-486.
    29.
  29. Leung A.Y. 1980. Encyclopedia of common natural ingredients used in food, drugs, and cosmetics: Wiley Publicaion.
    30.
  30. Longe A., Momoh J., Adepoju P. 2015. Effects of cinnamon aqueous extract on blood glucose level, liver biomarker enzymes, hematological and lipid profile parameters in alloxan-induced diabetic male albino rats. European Scientific Journal, 11(3):418-426.
    31.
  31. 19Mehran M., Hoseini H., Hatami A., Taghizade M. 2016. Investigation of components of seven Species of Thyme Essential oils and comparison of their antioxidant properties. Journal of Medicinal Plants, 2(58):134-140.
    32.
  32. Qiu C., Kivipelto M., von Strauss E. 2009. Epidemiology of Alzheimer's disease: occurrence, determinants, and strategies toward intervention. Dialogues in Clinical Neuroscience, 11(2):111-128.
    33.
  33. Rabiei Z., Mokhtari S., Asgharzade S., Gholami M., Rahnama S., Rafieian-Kopaei M. 2015. Inhibitory effect of Thymus vulgaris extract on memory impairment induced by scopolamine in rat. Asian Pacific Journal of Tropical Biomedicine, 5(10):845-851.
    34.
  34. Riedel B.C., Thompson P.M., Brinton R.D. 2016. Age, APOE and sex: triad of risk of Alzheimer’s disease. The Journal of Steroid Biochemistry and Molecular Biology, 160:134-147.
    35.
  35. Sa’adah N.N., Purwani K.I., Nurhayati A.P.D., Ashuri N.M., 2017. Analysis of lipid profile and atherogenic index in hyperlipidemic rat (Rattus norvegicus Berkenhout, (1769) that given the methanolic extract of Parijoto (Medinilla speciosa). AIP Conference Proceedings, AIP Publishing LLC.
    36.
  36. Setorki M., Mirzapoor S. 2017. Evaluation of Thymus vulgaris Extract on Hippocampal Injury Induced by Transient Global Cerebral Ischemia and Reperfusion in Rat. Zahedan Journal of Research in Medical Sciences, 19(5):e9216.
    37.
  37. Whitehouse P.J., Price D.L., Struble R.G., Clark A.W., Coyle J.T., Delon M.R. 1982. Alzheimer's disease and senile dementia: loss of neurons in the basal forebrain. Science, 215(4537):1237-1239.
    38.
  38. Wood H. 2015. Scanning ultrasound elicits amyloid-β clearance in mice. Nature Reviews Neurology, 11(5):247-247.
    39.

 

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