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  • Comparative Effect of Timolol and Methanolic Extract of Zataria multiflora with Vitamin E on APO E Gene Expression and Behavioral and Biochemical Changes in Alzheimer's Rats

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Manuscript ID : ASCIJ-2012-1221 (R2) Visit : 190 Page: 23 - 33

10.22034/ascij.2023.1917661.1221

Article Type: Original Research

Comparative Effect of Timolol and Methanolic Extract of Zataria multiflora with Vitamin E on APO E Gene Expression and Behavioral and Biochemical Changes in Alzheimer's Rats

Subject Areas : Journal of Animal Biology

Samira Homayoun Pour 1 (Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran)
Maryam Bananej 2 (Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran)
Maryam Khosravi 3 (Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran)
Hengameh Alibeik 4 (Department Biology, North-Tehran Branch, Islamic Azad University, Tehran, Iran)

Received: 2022-02-13 Accepted : 2022-05-16 Published : 2023-05-22

Keywords: vitamin E, methanolic extract, Zataria multiflora, Alzheimer's Disease, Thymolol,

Abstract :

Alzheimer's Disease is the most common form of dementia, characterized by memory and cognitive deficits. Vitamin E, as an antioxidant, plays an important role in reviving free radicals and converting them into safe substances by giving hydrogen. Shirazi thyme (Zataria multiflora) has antibacterial, antifungal and antioxidant properties. The aim of this study is the comparative effect of timolol with methanol extract of Zataria multiflora with vitamin E on Alzheimer's disease in male Wistar rats. 40 male Wistar rats weighing approximately 200 grams were randomly divided into 5 groups of eight. Beta-amyloid 42 was used to induce Alzheimer's disease. Then, methanolic and timolol extracts were extracted from Zataria multiflora. The level of Apo E gene expression was evaluated by Real Time PCR and TC, LDL and HD biochemical factors. The expression of Apo E gene in the experimental groups increased significantly compared to the Alzheimer's group. The expression of this gene was decreased in the sham group compared to the control group. The group of Alzheimer's mice had a significant increase in total cholesterol (TC) and low-density lipoprotein (LDL) serum levels, and the level of HDL showed a significant decrease compared to the control group. Timolol, m ethanolic extract of Zataria multiflora and vitamin E have positive effects on the expression of APOA E gene in leukocytes. On the other hand, with a significant effect on HDL, the methanolic extract of Zataria multiflora can be used as an effective product to prevent and reduce the complications of Alzheimer's disease.  

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