Comparison of the Effects of Flaxseed and Nigella Sativa Oils on Liver Enzymes and Some of the Inflammatory Factors in Male Rats with non-Alcoholic Fatty Liver

Subject Areas : Journal of Animal Biology

Mozhgan Rahimi ¹ , Seyed Ebrahim Hosseini ² , Mohammad Amin edalatmanesh ³

1 - Department of Biology, Shiraz Branch, Islamic Azad University, Siraz, Iran
2 - Department of Biology, Shiraz Branch, Islamic Azad University, Siraz, Iran
3 - Department of Biology, Shiraz Branch, Islamic Azad University, Siraz, Iran

Received: 2019-12-12 Accepted : 2020-06-03 Published : 2020-06-20

Keywords: liver enzymes, Fatty liver, N. sativa and flaxseed oils, Interleukin 6,

Abstract :

Fatty liver is a common metabolic disorder affecting millions of people all over the world. Due to the side effects of most of the chemical drugs used to treat this disease, this study has been performed aiming to investigate the effect of flaxseed and N. sativa oils on serum levels of liver enzymes and some of the inflammatory factors in in male rats affected by non-alcoholic fatty liver disease (NAFLD). In this experimental study, 40 mature male rats were randomly assigned to 5 groups (8 rats in each group) include the control group (receiving no treatment), fatty liver (treated by fat emulsion), and the experimenta groups under 6-weeks by fat emulsion, and 28-day treatments by 0.75 ml/kg of flaxseed oil, 5 ml/kg of N. sativa oil, and the combined treatmnet of 0.75+ 5 ml/kg of flaxseed and N. sativa oils, respectively. At the end, after blood sampling and separation of the surface serum of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), BUN (blood urea nitrogen), creatinine, total protein, and interleukin 6 were measured.  Data analysis was done by ANOVA and Tukey tests. The results showed that iduction of fatty liver caused significant increase of interleukin-6, total protein, blood urea nitrogen, ALP, ALT, AST enzymes, and creatinine compared to the control group (P˂0.01); while the use of flaxseed and N. Sativa oils alone and their combination caused a significant decrease in the level of the above mentioend (P˂0.01). factors The results showed that N. sativa and flaxseed oils and their combination can decrease the inflammatory serum factors and liver damage caused by fatty liver disorder.

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