The Study Biocompatibility Bone Marrow Stromal Cells by Interactivity Scaffold of Chitosan/Poly Ethylene Oxide
Subject Areas : Journal of Animal Biology
زهره یوزباشی
1
,
غلامرضا کاکا
2
,
ویدا حجتی
3
,
عبدالحسین شیروی
4
1 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 - گروه آناتومی، مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران
3 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
4 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
Keywords: Chitosan, scaffold, Poly ethylene oxide, BMSCs,
Abstract :
Several studies have been performed to achieve a BioCompatibility scaffold with stem cells. The aim of this study was to present biodegradable scaffold of chitosan (Cs)/poly ethylene oxide (PEO) and studies to evaluate the ability of growing, proliferation, undifferetation and cell death of bone marrow stromal cells (BMSCs) on them. At first, Cs/PEO scaffold made in 3, 1 ratio. This scaffold lies on 1% gelatin at 24 plates and then sterilized. BMSCs extract from adult rats femur and after three passages were implated in the empty house plate as control BMSCs and also on scaffold. The rate of cell proliferation, undifferentiation, and cell death were studied at two, four and six days after 3rd passage in plate. The results showed that the morphology of cells maintained on Cs/PEO scaffold and was simiar to control group. Studies showed that the percentage of differentiation of BMSCs and cell death on scaffold of six day was similar to control. Proliferation, undifferentiation and cell death of BMSCs on biodegradable Cs/PEO scaffold result a model of scaffold that can be used in tissue engineering and cell therapy.