Determination of Seizure Threshold due to Intrapritoneal Injection of Harmaline in NMRI Mice

Subject Areas : Journal of Animal Biology

غلامحسن واعظی ¹ , مهسا ترابی ² , عبدالحسین شیروی ³

1 - دانشیار گروه علوم جانوری دانشگاه آزاد اسلامی واحد دامعان
2 - کارشناس ارشد فیزیولوژی جانوری
3 - استادیار دانشگاه آزاد اسلامی واحد دامغان

Received: 2014-06-23 Accepted : 2017-05-21 Published : 2010-04-21

Keywords: Mice, PTZ, Harmaline, Seizure Threshold, Beta carboline, Peganum harmala, Alkaloids,

Abstract :

Harmaline is one of the alkaloids that exist in different plant like Peganum harmala. This alkaloid has stimulative effect on realasing serotonin and katkol -amins in different parts of the brain.Also this alkaloid has hallucinogenic effect. one of the most important pharmacological effects of Harmaline is the inhibitory effect on Monoamine oxidase enzyme.Also Harmaline with attachment to Inverse agonist locus GABA-A receptors produced a wide range of antagonistic effects against benzodiazepins and interference of benzodiazepine receptors in produce seizure and tremor has show.This research has done to estimate seizure threshold due to intrapritoneal injection of Harmaline in comparison with seizure threshold pentylenetetrazole (PTZ) of male NMRI mice.In this research the positive control receive of amount 45mg/kg,ip of PTZ and the other groups were injected with 5,10,20 mg/kg,ip of Harmaline.After above injections, seizure symptom has been estimated.As result of this research indicate the seizure threshold in positive group (PTZ) is amount 45mg/kg and seizure threshold of Harmaline is amount 5 mg/kg.In conclusion can reveal that Harmaline with attachment to Inverse agonist locus GABA-A receptors produced a wide range of antagonistic effects against benzodiazepins and has show that gabaergic system and serotonin neurotransmitters are involved in seizure activity due to Harmaline.While in seizure due to PTZ,interfere picrotoxine locus of GABA receptor and benzodiazepine locus has partial role on its effects.The result of this research has been shown that Harmaline reduce seizure threshold dose dependent significantly.