The Effect of Embryonic Cartilaginous Tissue Extract on Differentiation of Mesenchymal Stem Cells to Osteoblasts
Subject Areas : Journal of Animal Biology
مرضیه رمضانی
1
(دانشگاه آزاد اسلامی، واحد دامغان، گروه زیستشناسی، دامغان، ایران)
رمضان خان بابایی
2
(دانشگاه آزاد اسلامی، واحد قائمشهر، گروه زیست شناسی، قائمشهر، ایران)
ویدا حجتی
3
(دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران)
Keywords: mesenchymal stem cell, tissue extraction, Mouse embryo, cell differentiation, Osteoblast,
Abstract :
Allstem cells are originated from both embryonic stem cells and adult stem cells. The most important adult stemcellsaremesenchymal stem cells. Mesenchymal stem cellsare pluripotentandnon-hematopoietic cellswithahighdifferentiation potential.There arein thebone marrowand otherskeletal tissues.Mesenchymalstem cellsderivedfrombone marroware capabledifferentiationto osteoblast, osteocyte,adipocyte, chondrocyte, tendonsand muscle cells underin vivoandin vitro.In vitro osteoblastic differentiation needs to a variety of factors, which creates condition similar to bone tissue microenvironment. In this study, the effects of embryonic cartilaginous tissue extract was studied to differentiation of bone marrow mesenchymal stem cell into osteoblast. Mesenchymal stem cells were isolated from mice bone marrow and cultured in flasks containing DMEM-F12, 100 andmu;g/ml streptomycin, 100 U/ml penicillin and 10% FBS.Mesenchymal stem cellswerepurifiedbyseveral passages, then cellswere affectedby 15day mouse cartilaginous tissue extraction. After21 days ofculture, Alizarin Red staining was usedfor the detection ofosteoblasticcells.Aftereffect of extraction, spindle-shaped mesenchymal stem cellschange theirshapeAndmany appendages was observedincells. Alizarin Red staining has shown osteoblasticdifferentiation ofmesenchymal stem cells.Also osteoblastic differentiation of mesenchymal stem cells has confirmed morphogenic and osteogenic factors at cartilaginous tissue extracts of 15-day mouse embryo. Theresults express of osteoblastic differentiation of mesenchymal stem cells.Therefore, it is used in vitro models of osteoblast differentiation and genetic diseases of bone.