Cytotoxic Effects of Myrtus communis Extract on Hela and MCF7 Cancer Cells

Subject Areas : Journal of Animal Biology

سحر حبیب زاده خامنه ¹ (دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران)
فرخنده نعمتی ² (دانشگاه آزاد اسلامی، واحد قائم شهر، مرکز تحقیقات سلولی - مولکولی، گروه زیست‌شناسی، قائم شهر، ایران)
عبدالحسین شیروی ³ (دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران)

Received: 2014-06-18 Accepted : 2017-05-21 Published : 2014-01-21

Keywords: Cytotoxicity, ethanolic extract, Myrtus communis, Hela cell line, MCF7 cell line,

Abstract :

This study aimed to investigate the effects of Myrtus communis extract in 10, 7.5, 5, 2.5, 1.25, 0.625, 0.312, 0.156 mg/ml concentration on Hela and MCF7 cell lines after 72 h using the MTT assay. Hela and MCF7 cells were cultured in RPMI1640 medium containing fetal bovine serum, penicillin and streptomycin and incubated at 37 anddeg; C atmosphere containing 5% CO2 and 95% humidity in sterile flasks. Results show that ethanolic extract of M. communis in 1.25 mg/ml (p andle; 0.05), and 0.312, 0.156 (p andle; 0.02) concentration, was significantly reduced Hela cell growth compared to control, after 72 h. The highest percentage of growth inhibition at 0.625 mg/ml concentration was % 82.33. For MCF7 cells, ethanolic extract of M. communis in 2.5, 1.25, 0/312 mg/ml (pandle; 0/02) and 0.625 (pandle; 0/001) concentration was significantly reduced MCF7 cell growth compared to control, after 72 h. The highest percentage of growth inhibition at 1.25 mg/ml concentration was 70.64 %. The results suggest that ethanolic extract of M. communis has inhibitory effects on cell growth both on Hela and MCF7 cell line.