Antibacterial activity of methanol extract of some Iranian lichens

Subject Areas : Agroecology Journal

Soleiman Jamshidi ¹ (Young Researchers and Elite Club Miyaneh Branch Islamic Azad University Miyaneh, Iran)
Seyyedeh Maryam Shahidi ² (Young Researchers and Elite Club Miyaneh Branch Islamic Azad University Miyaneh, Iran)
Mohammad Sohrabi ³ (Professor assistant of Iranian Research Organization for Science and Technology (IROST) Tehran, Iran)
Samira Jamshidi ⁴ (Islamic Azad University Miyaneh, Iran)

Received: 2013-09-25 Accepted : 2014-05-15 Published : 2014-05-22

Keywords: antibacterial activity, bactericide, bacteriostatic, biocontrol, Natural compounds, antimicrobials,

Abstract :

Lichens are known as one of the greatest sources of natural compounds having antibiotic properties which some of them are being used as drugs for special diseases therapy. In current study, inhibitive, bacteriostatic and bactericidal activities of methanol extracts of five lichens derived from Arasbaran, East Azarbaijan Province of Iran including Pleopsidiumgobiensis, Parmelinatiliacea, Anaptychiasetifera and Lecanoraargopholis on some plant derived bacteria such asPseudomonas fluorecens, Bacillus subtillis and Enterobactersp. were studied using disc diffusion and minimal inhibitory and bactericide concentration methods. Methanol extracts of lichens had significant inhibitive effects of studied bacteria other than Enterobactersp. in disc diffusion agar method. A. setifera extracts had less inhibitive in bacteria than others. All lichens extracts had more or less bacteriostatic and bactericidal effects on bacteria. Methanol extracts of all lichens had various bacteriostatic and bactericidal effects on bacteria P. gobiensis and P. tiliaceahad much more bacteriostatic and bactericide comparing two other lichens. Lichens extracts was more inhibitive against B. subtillis. The bacteriostatic and bactericidal reaction of Enterobactersp. to lichen extracts was more than two other bacteria. Regarding results of the results, the lichens extracts could have remarkable potential for studied plant bacteria biocontrol and might be considered as promising agents against pathogens.

References:

1. Ahmadjianm, V. and hale, M.E. 1973. Methods of isolating and culturing lichen symbionts andthalli. In The Lichens, Academic Press, London. pp. 653–659.


2. Alexopoulos, CJ, Mims, CW and Blackwell M. 1996. Introductory on Mycology. 4^th edition. John Wiley, New York. 869 pp.


3. Anke, H., Kolthoum, I., Zahner, H. and Laatsch, H. 1980. Metabolic products of microorganisms. 185. The anthraquinones of the Aspergillusglaucus group. I. Occurrence, isolation, identification and antimicrobial activity. Arch Microbiol. 126(3):223-30.


4. Aslan, A, Gulluce, M, Atalan, E. (2001). A study of antimicrobial activity of some lichens. Bull. Pure Applied Science 20: 23-26.


5. Aydin S, Kinaliogli K. (2010). Antimicrobial activity of the lichen extracts of Pseudeverniafurfuraceavar. furfuracea and P. tiliaceae. The Black Sea Journal of Sciences Sonbahar 1(2): 30-38.


6. Boustie J, Grube M. (2005).Lichensa promising source of bioactive secondary metabolites. Plant Genetic Resources: Characterization and Utilization 3(2): 273-287.


7. Burkholder P, Evansa W, Mcveigh I, Thorntonh K. (1994). Antibiotic activity of lichens. Proceeding of National Academic Science, USA, 30: 250–255.


8. Dulger B, Gucin F, Kara A, Aslan A. (1997). Useneaflorida (L.) Wig. antimicrobial activity of lichen, Transactions Journal of Biology 21:103-108.


9. Elix JA, Stocker-Wörgötter E. (2008). Biochemistry and secondary metabolites. - In: T. H. Nash, (ed.) III: Lichen Biology. Second Edition. Cambridge University Press, Cambridge UK.104-133. 


10. Esimone, C.O. and Adikwu, M.U. 1999. Antimicrobial activity and cytotoxicity Ramalinafarinacea. J. Fitoterapia, 70: 428-431.


11. Houshyar F, Jamshidi S, Sorhrabi M. (2013). Antibacterial potential of five lichen species on Fusariumequisetiand Pectobacteriumcarotovorapv. carotovorapotato rot agents in laboratory and storage condition. The conference of New Topics in Agriculture 14 pp.


12. Huneck S. (1999). The Significance of lichens and their metabolites. Naturwissenschaften 86: 559-576.


13. Marijana, K., Rankovic, B. and Sukdolak. S. 2010. Antimicrobial activity of the lichen Lecanorafrustulosa and Parmeliopsishyperopta and their Divaricatic acid and zeorin constituents. African J. of Microbiology Research, 4 (9): 885-890.


14. Miao, V., Coeffet, M., Brown, D., Sinnemann, S., Donaldson, G. and Davies, J. 2001. Genetic approaches to harvesting lichen products. Trends Biotechnol., 19: 349-355.


15. Nash TH. (1996). Lichen biology. Cambridge University Press, Cambridge, UK. 303 pp.


16. ÖzdemirTürk, A., Yılmaz, M., Kıvanc, M. and Türk, H. 2003. The Antimicrobial activity of extracts of the lichen Cetrariaaculeata and its protolichesterinic acid constituent, Z. Naturforsch., 58c: 850-854.


17. Paudel, B., Bhattarai, H. D., Lee, H. K., OH, H., Bhin, H. W. and Yim, J. H. 2010. Antibacterial activities of Ramalin, Usnic Acid and its Three Derivatives Isolated from the Antarcitic Lichen Ramalinaterbrata. Z. Naturforsch., 65(1-2): 34-38.


18. Ranković B, Mišić M, Sukdolak S, Milosavljevic D. (2007). Antimicrobial activity of the lichens Aspiciliacinerea, Collemacristatum, Ochrolechiaandrogyna, Physiciaaipolia and Physciacaesia. Italian Journal of Food Science 19(4): 461-469.


19. Rankovic B, Rankovic D, Kosanic M, Msric D. (2010). Antioxidant and antibacterial properties of the lichens Anaptychyaciliaris, Nephromaparile, Ochrolechiatartarea and Parmeliacentrifuga, Central European Journal of Biology 5 (5): 649-655.


20. Romagni JG, Rosell RC, Nanayakkara NPD, Dayan FE. (2004). Ecophysiology and potential modes of action for selected lichen secondary metabolites. In: Macías FA,Galindo JCG, Molinillo JMG, Cutler HG (eds.): Allelopathy. Chemistry and Mode of Action of Allelochemicals. CRC Press, Boca Raton, Florida. pp. 13-33.


21. Sati, S.C. and joshi, S. 2011. Antibacterial activity of the Himalayan lichen Parmotremanilgherrense extracts, British Microbiology Research Journal, 1(2): 26-32.


22. Shahidi SM, JamshidiS,andTorabi M (2013a). Antibacterial potential of five lichen species derived from Arasbaran region on Dikeryachryanthemi potato in laboratory and storage condition. Modern Science of Sustainable Agricultural Journal. 8(4): 55-65.


23. Shahidi SM, Jamshidi S, andTorabi M (2013b). Inhibitive effect of five lichen species different extracts on Ralstoniasolanacearum. The 1st National Conference of Sustainable Development of Agriculture and Green Environment 9 pp.


24. Shahidi, SM, Jamshidi S and Torabi (2013c). Antibacterial potential of five lichen species from Arasbaran region against Pectobacteriumcarotovorapv. carotovora causing potato soft rot in laboratory and storage conditions Iranian Journal of Applied Plant Protection. In press.


25. Thippeswamy B, Sushma NR, Naveenkumar KJ. (2010). Antimicrobial property of bioactive factor isolated from Parmeliaperlata. International Multidisciplinary Research Journal 2 (2): 1-5.


26. Tomas HN. (2008). Lichen Biology. Arizona State University, USA, 498 pp.


27. Yilmaz M, Tay T, Kivanc M, Turk H, Turk AS. (2005). The antibacterial activity of extracts of the lichen Hypogymniatubulisaand its 3-hydroxyphysodic acid constituent. Z. Naturforsch 60c: 35-38.