Evaluation of chemical compounds in the extract (Urtica dioica L.) and compare the therapeutic effect of ciprofloxacin against common bacteria isolated from patients with urinary tract infection Hospital Vali-Asr Meshgin city
Subject Areas : The Application of Chemistry in Environment
Keywords: MIC, Urinary Tract Infection, Extract, HPLC, Antibacterial, Urtica dioica L,
Abstract :
Use of medicinal plants for the treatment of diseases of the past have been considered. the infectious disease is of considerable importance. according to Nettle antibacterial effects and side effects of chemical drugs, replacing them by antimicrobial drugs of plant origin is very important. after collecting the nettle plant phenolic compounds and extracts maceration method, was measured using HPLC, then to investigate the antimicrobial activity of two ways disk deffusion and MIC against common bacteria urinary tract infection in concentrations of 12/5, 25, 50 and 100 mg/ml with 3 replications. Phenolic compounds such as chlorogenic acid (1/28%), rutin (2.25%), myricetin (6/10%) and vanillic acid (2/9%) was obtained using HPLC. acinetobacter calcoaceticus with a diameter of 21 mm maximum diameter of acinetobacter and enterococcus faecalis with a diameter of 4 mm was the lowest. according to the results of the test MIC, enterococcus faecalis greatest sensitivity with a minimum inhibitory concentration of 5.1 mg / ml and enterobacter aerogenesis least sensitive to nettle extract of the herb. the extract of nettle can be another suitable alternative can be used to treat urinary tract infection. It is necessary to continue further studies and extensive in terms of invivo be done so that the plant extract, as an introduced new products.
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