Optimization of carotenoid pigment extraction from tomato using industrial hemicellulase enzyme

Subject Areas : The Application of Chemistry in Environment

arezoo  ghadi ¹ (chemical engineering)

Received: 2022-01-03 Accepted : 2022-04-07 Published : 2020-08-22

Keywords: carotenoids, Tomato powder, Hemicellulose enzyme,

Abstract :

In this study, the process of separating lycopene carotenoid pigment from tomato powder by industrial hemicellulose enzyme was studied. For this purpose, the effects of temperature change, enzyme concentration, incubation time, pH were all studied at optimum points. The research is based on the effectiveness of the enzyme in different conditions on the amount of pigments extracted from tomato powder and obtaining the optimal extraction points by statistical analysis of the results. To extract the pigment in this study, solvents of hexane, ethanol, acetone in a ratio of 1: 2:1 and incubation at 30 ° C for 16 hours were used for the full effectiveness of the solvent. For each variable, three levels were defined. In order to target the experiments, Taguchi design was used for the experiment design table and included nine experimental rows. For each experimental row, three replications and a control containing an enzyme-free sample were considered. Pigment-containing solutions were transferred to spectrophotometry at the end of the separation stage and after recording the data and statistical analysis, it was observed that the effectiveness of the enzyme concentration variable was much higher than other variables, so that the highest average pigment extraction concentration at 500 μg = .0.450 Separately, each variable has optimal points for the incubation temperature of 40 degrees and for the incubation time of 60 minutes, for pH = 4, the enzyme concentration is 200 micrograms per liter for every 5 grams of tomato powder.