Antibacterial Effects of Polar, Semi-Polar, Non-Polar Fractions of Aractium Lappa Extract On Staphylococcus Aureus, Escherichia Coli, Pseudomonas Aeruginosa, Listeria Monocytogenes

Subject Areas : The Application of Chemistry in Environment

changiz ahmadizadeh ¹ , leili rahimi ² , rohollah safari ³

1 - islamic azad university
2 - islamiv azad university
3 - islamic azad university

Received: 2022-10-11 Accepted : 2022-10-12 Published : 2021-09-23

Keywords: antibacterial effect, plant extract, fractions, Aractium Lappa,

Abstract :

Aractium Lappa one of the daisies of dicotyledons in Iran. It is antioxidant, antimicrobial, anti-cancer and anti-rheumatic and lowers blood glucose. In this paper, after extraction with ethanolic, chloroform and n-hexane solvents with soxhlet and after evaporation of solvent with rotary device, studies were conducted on inhibition zone, Minimum Inhibitory Concentration (MIC) and MBC. Studies on MIC and MBC of Aractium Lappa extract were performed on 4 strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Listeria monocytogenes.MIC values indicated that the ethanolic, chloroform and n-hexane extracts of Aractium Lappa are 12.5-25.6%, 25.6-50% and 50-100%, respectively. Ethanolic extract has the highest activity against Listeria monocytogenes and Pseudomonas aeruginosa at a concentration of 25.6% and the lowest effect was against E. coli and of Staphylococcus aureus at 5.12%.MBC for ethanolic extract in Staphylococcus aureus and Pseudomonas aeruginosa is 5.12% and in Escherichia coli and Listeria monocytogenes is 25% and 25.6%, respectively. The results showed that the extract of Aractium Lappa has an antibacterial effect