Background: The purpose of this research was to compare the velocity of the dominant hand and the non-dominant hand in the Gyakozuki technique (with hit and without hit) of elite karate men.Methods: 16 Iranian male karatekas with individual characteristics from the national karate team camp participated in this study. The subjects were asked to perform the Gyakuzuki technique with the dominant hand and the non-dominant hand five times with maximum intensity and strength, hit the shoot fist device. The distance of standing from the device was equal to the length of the upper limb, and the best record of all five performances was considered. It was recorded using a Hero3 digital camera made in the United States (240Hz) in two dimensions. It was filmed at a perpendicular angle to the subject. The peak velocity of the joints (shoulder, elbow, wrist) and the velocity at the moment of the collision were evaluated using Skillspector software. After ensuring the normality of the data using the Shapiro–Wilk test, independent t-tests were used at the level of (p ≤ 0.05) with spss version 22 software.Result: The test results showed a significant difference between peak joint velocity shoulder, elbow, and wrist withthe dominant and non-dominant hand in the with-hit and without-hit method ( p ≤0.05). Also, there was a significant relationship between the velocity of the wrist in contact moment with the dominant and non-dominant hand in the with-hit and without-hit methods ( p ≤0.05).Conclusion: According to the fest result, if a karate man wants to reach to velocity peak with the dominant hand in Gyakuzuki Techniques, he should reinforce the antagonist and stabilizer muscles in the non-dominant hand. Manuscript profile