A microchannel device was designed and utilized to separate Michigan Cancer Foundation-7 cells from white blood cells using both wall-induced lift and di-electrophoresis forces. Using COMSOL Multiphysics simulator, the label-free separation process is performed based on the radius size of the particles. The best performance of the structure is obtained with the efficiency of 100% and 99% for cancer and white blood cells separation processes, respectively. For the proposed microfluidic structure, in addition to the highest available efficiency and high separation speed of 12 um/s, the low DC voltage of 6 V is applied, which causes negligible damage to the blood cells. Manuscript profile