Electrospinning has been recognized as an efficient and simple technique for the fabrication of polymer nanofibers for biofunctionals. Recently, various polymers have successfully been electrospun into ultrafine fibers. Electrospinning is an extremely promising method for the preparation of tissue engineering scaffolds. In this study, nanofibers gelatin was electrospun at 20% v/v optimized content. To produce gelatin nanofibers optimally, production parameters need to be investigated. In the electrospinning, device (voltage and distance) parameters were determined to be effective; as a result, these parameters were researched and the influences of electrospinning device parameters (voltage & distance) on properties of gelatin nanofibers were evaluated. These parameters affected the diameter size, uniformity, hydrophilicity and thermal degradation of electrospun gelatin nanofibers. All of these properties were examined by SEM, FTIR, CA, BET, XRAY and TGA tests and finally optimum gelatin nanofibers can be used in many applications including cell culture, drug delivery and tissue engineering. Manuscript profile