For the therapeutic application and drug delivery of AgNPs in medicine, pharmacy and cosmeticproducts, it is essential to know the distribution and local or systemic toxicity associated with them.For this purpose, this study was carried out to assess the potential consequences of skin injection ofnanochelating based synthesized AgNPs on the mice models. Nanochelating technology used to designand synthesize the AgNPs. The histopathological findings in skin and tissue of micemodels have assessedvia histopathological analysis.All samples were visualized by an independent pathologist. The results ofeach sample have reported as follows, micrograph of the skin cells have shown the normal architectureand cells in all samples. Moreover, histopathological evaluation of samples have shown normal withoutany significant pathological changes compared to control groups in volume of derm and epiderm, andthe number of fibroblast, neutrophil, and macrophage.In summary, this study has observed no obviousdecline of immunological performance and morphological signs of skin damage in the mice caused bynanochelating based AgNPs exposure. These findings could provide a fundamental understanding of theintrinsic toxicity associated with nanochelating based AgNPs on biological models. Moreover, this studywould arisetypical attentions on the future applications of nanochelating based AgNPs on human, whichis valuable for short-term and low dose treatment in nanomedicines. Manuscript profile