The phytochemical study of the methanolic extract of the stem bark of Parkia bicolor A. Chev. (Leguminosae) led to the isolation and characterization of ellagic acid (1), 3,3’-dimethoxyel lagic acid (2), 3,3’,4,4’-tetramethoxyellagic acid (3), 3-glucopyranoside-3’-methoxyellagic acid (4), gallic acid (5), methylgallate (6), oleanolic acid (7), erythrodiol (8), β-amyrin (9), β-sitosterol (10), stigmasterol (11) and stigmasterol-3-O-β-D-glucopyranoside (12). The structures of these compounds were determined by spectroscopic analyses of 1D and 2D NMR and EI- and ESI-MS and comparison with the reported data. Compounds 1-4 are reported here from this species for the first time. The crude methanolic extract and isolated compounds 1-6 were submitted to micro dilution assay against the Gram negative bacteria Escherichia coli ATCC 8739 and Salmonella typhi clinical strain as well as the Gram positive bacterium Staphylococcus aureus clinical strain. While the methanolic extract showed moderate to significant antibacterial activ ity against above tests strain with MICs in the range of 64-128 μg/mL, compound 4 displayed the best activity against Salmonella typhi and Staphylococcus aureus clinical strains with an MIC of 33.4 μM against both, compared to gentamycin with 0.1-2.0 μM. Compounds 1-3 and 5, 6 showed moderate to weak antibacterial activity with MICs in the range of 44.6 ± 0.9 to 376.4 ± 1.5 μM. MBCs were above the MICs in the range of 128-512 μg/mL for the methanolic extract resulting in MBCs/MICs in the range of 2 to 8. In antifungal assays, the methanolic extract when tested at 50 mg/mL gave growth inhibition of 34.3, 31.3, 28.3, 33.1, 30.6 and 22.4 % against Aspergillus flavus strain 1, Aspergillus flavus strain 2, Aspergillus niger, Aspergillus gandidus, Ab sidia sp < /em>. and Penicillium sp < em>., respectively. When tested at 2 mg/mL, 4-glucopyranoside-4’-me thoxyellagic acid (4) showed a moderate inhibition potency against all the fungal strains with growth inhibition of 24.50 to 40.40% compared to fluconazole with 100% at 2 mg/mL. Manuscript profile

One fatty acid (1), one monoglycol ester (2), three diterpenes (3-5), four steroids (6-9), four triterpenes (10-13), three flavonoids (14-16), three anthraquinones (17-19) and one benzoquinone (20), were isolated from the methanolic extract of the leaves and trunk of Senna alata. These compounds were obtained by extensive silica gel chromatography and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) as well as comparison with literature data. Compounds (2-4) have been reported for the first time from this species. Some isolated compounds and methanolic crude extract of leaves and trunk were screened in vitro for their antimicrobial activities. Kaempferol (16) exhibited strong activity against all the tested strains, with MIC values varying from 15.5 to 31.2 µg/mL, emodin (18) was active with a strong activity (7.8 µg/mL) exhibited on Pseudomonas aeruginosa. The trunk crude extract showed a strong activity with MIC values varying from 15.6 to 62.5 µg/mL. Manuscript profile