This study intends to draw a structural model of job satisfaction from the perspective of Herzberg’s hygiene-motivation theory. The study was conducted on 528 staff of Research, Education and Extension Organization (AREEO), Iran. The sample size was determined by the Cochran method (n=222) and the data collection tool was a questionnaire which was a combination of Minnesota questionnaire and researcher-made. Panel of experts and Cronbach's alpha coefficient (0.89) indicated the validity and reliability of the questionnaire. Descriptive and inferential analyses were performed using SPSS software. According to the results of the correlation, the effect of individual characteristics on staff job satisfaction showed that 10% of the variance (variance) of staff job satisfaction was explained by them. Average satisfaction with hygiene factors and average satisfaction with motivational factors of AREEO staff were lower than average the job satisfaction rate. Amos software tested the theoretical model of research that showed that job satisfaction has both motivational and hygiene factors in the target population. The unique result of this paper was extracted the fitting model that indices were used to check the fit of the model (RMSEA=0.137; GFI=0.808; CMIN; 145.297), and the fitting values obtained for the research model as a whole confirm the suitability of the model. Manuscript profile