A simple, economical, precise and fast visible spectrophotometric method has been developed for the determination of Azathioprine in tablet dosage form. Developed method is based on the formation of extractable colored complex of drug with colouring agent Indigo Carmine and Methyl orange. A wavelength maximum was found to be 683.7 nm and 566 nm. The concentration range of 15-50 μg mL-1 with of percentage recovery 100.05-101.31 %, while the linear regression, LOD and LOQ were 99.42-99.08 %, 0.38 and 0.84 μg mL-1 and 0.74 and 0.18 μg mL-1, respectively. Two new sensitive spectrophotometric methods are proposed for the determination of Azathioprine using bromate-bromide mixture and two dyes, methyl orange and indigo carmine, as reagents. The methods entail the addition of a known excess of bromate-bromide mixture to Azathioprine, in acetic acid medium followed by determination of residual bromine by reacting with a fixed amount of either indigo carmine and measuring the Absorbance at 683.6 nm (Method A) or methyl orange measuring the absorbance at 566 nm (Method B). Manuscript profile

Two simple, accurate and precise methods for simultaneous estimation of Esomeprazole and Itopride in bulk drug and tablet dosage form have been described. Method-A employs formation and solving of simultaneous equation using 231 and 370 nm as two analytical wavelengths. Method -B is Absorption ratio method, which uses 308 and 331 nm as two analytical wavelengths.Beer’s law was obeyed in the concentration range 5-35 μg/ml for Esomeprazole and 5-40 μg mL-1 for Itopride. In the present investigation, 0.5 Metformin hydrochloride solutions (hydrotropic solubilizing agent) were employed to solubilize, Esomeprazole and Itopride from fine powder of its tablets to carryout Spectrophotometric analysis. The optimized methods showed good reproducibility and recovery with standard deviation of < 1.0% and percent relative standard deviation less then 2.0%, allow the simultaneous estimation of Esomeprazole and Itopride in concentration ranges employed for this purpose in the assay of bulk drug and tablets. Manuscript profile