Background & Aim: The major problem of pharmaceutical industry for herbal product in current scenario is authentication of raw material, and availability of standards. The quality control and quality assurance of herbal drugs still remains a challenge because of the high variability of chemical components involved. Experimental: In present research work details of Trisama formulationwere noted including preliminary pharmacognostical study. The physicochemical and phytochemical evaluation of Trisama formulation was done using various laboratory chemicals and reagents. Results:Microscopical characters like boarder pitted vessel, brown content, compound starch grain, cork in surface view, stone cell, lignified collenchyma cell, sclerides, scleriform vessel etc are seen. Physicochemical and phytochemicals parameters also performed in both dosage forms however difference is seen in parameters result. Still both the dosage forms fulfill the minimum qualitative standards at a preliminary level. The following study will improve the quality of drugs as well as help to get more involved in the standardizations of formulations.Recommended applications/industries: The abundant chemical constituents and significant potential could be well and new source of unexplored Ayurvedic formulation in industry. Manuscript profile