Background: The present study aimed to use a DIAGNOdetnt unit to compare the effects of casein phosphopeptideamorphous calcium phosphate fluoride (CPP-ACPF), 0.05% sodium fluoride (NaF) mouth rinse, and glass ionomer (GI) on enamel remineralization.Materials and Methods: This in vitro study was performed on 50 intact human premolars in five groups: A (artificial saliva), B (CPP-ACPF), C (NAF), D (GI), and E(GI+NAF). All samples were assessed using a DIGNOdetnt unit before and after demineralization. The teeth in each group were treated (except for the control group) then pH cycled repeatedly for 30 days. After this, the remineralization evaluation was evaluated. Data gathered by DIAGNOdetnt were analyzed by One way analysis of variance. The least significant difference (LSD) test was used for multiple comparisons (α=0.05).Results: After the treatment, the mean DIAGNOdetnt values in groups B(3.6), C(4.7), D (5.1), and E(3.5) were significantly lower than that in group A(P<0.0001). LSD test showed that the mean DIAGNOdetnt value in groups B and E was lower than for groups C and D(P<0.001).Conclusion: Although the repeated application of all the experimental materials was efficient, CPP-ACPF and GI+NAF had more prominent remineralization potential in comparison to NAF and GI alone. Manuscript profile