Purslane is a medicinal herb that is used as an antioxidant mostly because of its secondary metabolites. One of the most important biotechnological methods for changing the production pattern of secondary metabolites is the elicitation of cell suspensions. In the present study, the effects of JA (Jasmonic Acid ) and SA (Salicylic acid ) growth hormones were investigated on bioactive compounds of Portulaca oleracea L. suspension culture. Stem segments were cultured in MS medium with BAP and 2,4-D for callus induction. Cell suspensions were initiated from the callus and after elicitation with JA and SA, metabolites were extracted. Antioxidant capacity, total phenol, flavonoids, saponins, alkaloids and terpenoids were measured in the treated groups. Concomitant use of JA and SA increased antioxidant capacity compared to individual treatments. The increase in total phenol and flavonoids was almost proportional to the increase in antioxidant capacity. The amount of saponin in combined JA treatments (250 μM) with both concentrations of SA was the same as in single SA treatments. JA and SA combination treatment at both concentrations had the same effect on alkaloid production. The highest amount of terpenoids was induced with SA treatment with a concentration of 50 μM. Simultaneous treatment of P. oleracea L. suspension with JA and SA, increased antioxidant capacity by increasing the bioactive compounds of total phenol, alkaloids and terpenoids Manuscript profile