Investigating the effect of swimming exercise and vitamin D consumption on B-catenin/TCF signaling pathway in endometriosis model rats

Subject Areas : Role of Genes in Health

Shahrbano  jaafari ¹ (Ph.D Candidate, Department of Physical Education and Sports Science, Sari Branch, Islamic Azad University, Sari, Iran)
hajar  abbaszadeh ² (Associate Professor, Department of Physical Education and Sports Science, Sari Branch, Islamic Azad University, Sari, Iran)
parvin  farzanegi ³ (Associate Professor, Department of Physical Education and Sports Science, Sari Branch, Islamic Azad University, Sari, Iran)
Leila  zameni ⁴ (Assistant Professor, Department of Physical Education and Sports Science, Sari Branch, Islamic Azad University, Sari, Iran)

Received: 2023-05-28 Accepted : 2023-07-06 Published : 2023-05-22

Keywords: Swimming, vitamin D, β-catenin, Endometriosis, TCF,

Abstract :

The aim of the present study was to investigate the effect of swimming exercise and vitamin D consumption on β-catenin /TCF signaling pathway in endometriosis model rats. In this way, the number of 30 8-week-old male Wistar rats with an average weight of 202.85±15.62 grams after induction of the model by transplanting the tissue around the endometrial region randomly into 6 groups; healthy control(n=5), Sham (normal saline solution to control the effect of eating the gavage)(n=5), endometriosis(n=5), endometriosis+vitamin D(n=5), endometriosis+swimming(n=5), endometriosis+swimming+ vitamin D(n=5). The amount of vitamin D consumption was 200 mg/kg per body weight of each rat, and the swimming training program was for 8 weeks, five days a week, and every day for 30 minutes. One-way analysis of variance was used to analyze the data. The findings showed that the expression level of β-catenin and TCF genes was significantly higher in the endometriosis group than in the healthy control group (p=0/007, 0/035).Swimming, vitamin D and a combination of swimming+D were associated with a decrease in β-catenin and TCF gene expression.In general, the results of this research indicate that the regulation of β-catenin and TCF gene expression in a normal state in the process of endometriosis can improve the level of this disease, and possibly swimming exercises and taking at the same timevitamin D will be effective in the normal state of the β-catenin/TCF pathway.