Morphological and phytochemical characteristics of Inula helenium L. in different altitudes of Zanjan region, Iran

Subject Areas : Medicinal Plants

Nader  Moradi ¹ (Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
Hassanali  Naghdi Badi ² (Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran.)
Sepideh  Kalateh Jari ³ (Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
Ali  Mehrafarin ⁴ (Medicinal Plants Research Centre, Institute of Medicinal Plants, ACECR, Karaj, Iran)
Elham  Danaee ⁵ (Department of Horticulture, Islamic Azad University, Garmsar Branch, Garmsar, Iran)

Received: 2020-12-03 Accepted : 2021-07-23 Published : 2022-12-22

Keywords: Altitude, growth traits, Zanjan Province, Essentials Oil, Inula helenium L,

Abstract :

In the present study, the effect of altitude on the morphological characteristics and also essential oil content and composition of Inula helenium L. was studied. This plant was collected in the flowering stage in the first half of July 2017 and 2018 in different altitudes of the Zanjan region (from 1200 to 2400 meters above sea level) and the growth traits of plant organs including leaves, stems, flowers, and roots were measured. In autumn, at the end of the annual growth period of the plants, plant rhizomes were harvested to evaluate essential oil content and composition. The Clevenger device was used for 3 hours for essential oil extraction and the GS-MS device was used to identify the constituents of Inula helenium essential oil. The results showed that there were significant differences in all growth traits and essential oil depending on the altitude of the region. Although, the growth traits of Inula helenium such as leaf and stem dry weight, leaf width and length, leaf number, and plant height decreased with increasing altitude, but the rhizome dry weight and diameter, and essential oil content increased with increasing altitude. The essential oil components of I. helenium were changed with increasing altitude so that the content of geranyl acetone, verbanol, α-pinene, isoalantolactone and valencene increased with increasing altitude. Also, the content of α-selinene decreased and no changes were observed in the content of caryophyllene oxide and β-elemene with increasing altitude. In general, the results showed that altitude is an important and influential factor affecting Inula helium's quantitative and qualitative yield.

References:

References

1. Aboukhalid, K., AL Faiz, C., Douaik, A., Bakha, M., Kursa, K., Agacka‐Mołdoch, M., Machon, N., Tomi, F. and Lamiri, A. 2017. Influence of environmental factors on essential oil variability in Origanum compactum growing wild in Morocco. Chemistry & biodiversity, 14: e1700158.


2. Arbabi, M., Naghdi Badi, H., Labbafi, M., Mehrafarin, A. and Saboki, E. 2020. Investigating the essential oil composition of Ducrosia anethifolia (DC.) Boiss. in differnet altitudes of Sistan and Baluchestan province, Iran. Journal of Medicinal Plants, 19: 341-353.


3. Azarnivand, H., Arabani, M. G., Sefidkon, F. and Tavili, A. 2010. The effect of ecological characteristics on quality and quantity of the essential oils of Achillea millefolium subsp. millefolium. Iranian Journal of Medicinal and Aromatic Plants, 25: 556-571.


4. Cabo, J., Crespo, M., Jimenez, J., Navarro, C. and Risco, S. 1987. Seasonal variation of essential oil yield and composition of Thymus hyemalis. Planta medica, 53: 380-382.


5. Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10 (3).


6. Chen, W., Li, P., Liu, Y., Yang, Y., Ye, X., Zhang, F. and Huang, H. 2018. Isoalantolactone induces apoptosis through ROS-mediated ER stress and inhibition of STAT3 in prostate cancer cells. Journal of Experimental & Clinical Cancer Research, 37(1): 1-12.


7. Chen, G., Sun, W., Wang, X., Kongkiatpaiboon, S. and Cai, X. 2019. Conserving threatened widespread species: a case study using a traditional medicinal plant in Asia. Biodiversity and Conservation, 28: 213-227.


8. Delbanco, A.S., Burgess, N.D. and Cuni-Sanchez, A. 2017. Medicinal plant trade in Northern Kenya: economic importance, uses, and origin. Economic Botany, 71: 13-31.


9. Diguță, C., Cornea, C., Ioniță, L., Brîndușe, E., Farcaș, N., Bobit, D. and Matei, F. 2014. Studies on antimicrobial activity of Inula helenium Romanian cultivar. Romanian Biotechnological Letters, 19: 9699.


10. Etehadpour, M. and Tavassolian, I. 2019. Ecological factors regulate essential oil yield, percent and compositions of endemic yarrow (Achillea eriophora) in Southeast Iran. International Journal of Horticultural Science and Technology, 6: 201-215.


11. Farhang, S.A., Kheyri, A., Soleymani, A. and Zibaseresht, R. 2014. Evaluation of morphological traits and oil contents of Achillea aucheri from different altitudes. Journal of Medicinal Plants and By-products, 2: 127-131.


12. Flamini, G., Cioni, P. L., Morelli, I., Maccioni, S. and Baldini, R. 2004. Phytochemical typologies in some populations of Myrtus communis on Caprione Promontory (East Liguria, Italy). Food chemistry 85: 599-604.


13. Gao, S., Wang, Q., Tian, X.-H., Li, H.-L., Shen, Y.-H., Xu, X.-K., Wu, G.-Z., Hu, Z.-L. and Zhang, W.-D. 2017. Total sesquiterpene lactones prepared from Inula helenium has potentials in prevention and therapy of rheumatoid arthritis. Journal of ethnopharmacology, 196: 39-46.


14. Hassanabadi, M., Ebrahimi, M., Farajpour, M. and Dejahang, A. 2019. Variation in essential oil components among Iranian Ferula assa-foetida accessions. Industrial Crops and Products, 140:111598.


15. Ivanova, V., Trendafilova, A., Todorova, M., Danova, K. and Dimitrov, D., 2017. Phytochemical profile of Inula britannica from Bulgaria. Natural product communications, 12(2):1934578X1701200201.


16. Jana, B.K., Bar, R. and Mukherjee, S. 2013. Cypselar morphology and anatomy of five species of the tribe Inuleae-Asteraceae. International Journal of Pharma and Bio Sciences, 4: 911-919.


17. Joshi, B.C.and Joshi, R.K. 2014. The role of medicinal plants in livelihood improvement in Uttarakhand. International Journal of Herbal Medicine, 1: 55-58.


18. Kheiry, A., baharmast, Z., Sanikhani, M. and Soleimani, A. 2020. Study and comparison of morphological and phytochemical traits of Mentha pulegium in different habitats of Guilan province. Eco-phytochemical Journal of Medicinal Plants (EJMP), 8(30): 60-75.


19. Kokkini, S., Karousou, R. and Lanaras, T. 1997. Essential oils with 1, 2‐epoxy‐p‐menthane derivatives from Mentha spicata plants growing across the Island of Crete. Botanica Acta, 110: 184-189.


20. Larsen, H.O. and Olsen, C.S. 2007. Unsustainable collection and unfair trade uncovering and assessing assumptions regarding central Himalayan medicinal plant conservation. Biodiversity and Conservation, 16:1679-1697.


21. Li, Y., Ni, Z.Y., Zhu, M.C., Dong, M., Wang, S.M., Shi, Q.W., Zhang, M.L., Wang, Y.F., Huo, C.H. and Kiyota, H. 2012. Antitumour activities of sesquiterpene lactones from Inula helenium and Inula japonica. Zeitschrift für Naturforschung C, 67: 375-380.


22. Mirza, M. and Sefidkon, F. 1999. Essential oil composition of two Salvia species from Iran, Salvia nemorosa and Salvia reuterana Boiss. Flavour and Fragrance Journal. 14: 230-232.


23. Morshedloo, M.R., Salami, S.A., Nazeri, V., Maggi, F. and Craker, L. 2018. Essential oil profile of oregano (Origanum vulgare) populations grown under similar soil and climate conditions. Industrial Crops and Products, 119: 183-190.


24. Nalawade, S.M., Sagare, A.P., LEE, C.Y., Kao, C.L. and Tsay, H.S. 2003. Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization. Botanical Bulletin of Academia Sinica, 44: 79-98.


25. Olennikov, D.N., Dudareva, L.V., Osipenko, S.N. and Penzina, T.Y.A. 2010. Chemical composition of Rhododendron aureum (gold rosebay) essential oil from Pribaikal'e (Russian Federation). Journal of the Serbian Chemical Society, 75: 209-215.


26. Özgüven, M. and Tansi, L. 1998. Drug Yield and Essential Oil of Thymus vulgaris as in Influenced by ecological and ontogenetical variation. Turkish journal of agriculture and forestry, 22(6): 537-542.


27. Rostaefar, A., Hassani, A. and Sefidkon, F. 2018. Effect of altitude on essential oil composition in different gender of Juniperus communis hemisphaerica growing wild in Amol. Eco-phytochemical Journal of Medicinal Plants (EJMP), 6(1): 1-11.


28. Royer, D.L., Wilf, P., Janesko, D.A., Kowalski, E.A. and Dilcher, D.L. 2005. Correlations of climate and plant ecology to leaf size and shape: potential proxies for the fossil record. American journal of botany, 92: 1141-1151.


29. Seo, J.Y., Park, J., Kim, H.J., Lee, I.A., Lim, J.S., Lim, S.S., Choi, S.J., Park, J.H.Y., Kang, H.J. and Kim, J.S. 2009. Isoalantolactone from Inula helenium caused Nrf2-mediated induction of detoxifying enzymes. Journal of medicinal food, 12: 1038-1045.


30. Shabankare, H.G., Asgharipour, M.R. and Fakheri, B. 2015. Morpho-chemical diversity among Iranian Teucrium polium(Lamiaceae) populations in Fars Province. Journal of Agricultural Science and Technology, 17: 705-716.


31. Shafie, M.S.B., Hasan, S.M.Z. and Shah, R.M. 2009. Study of genetic variability of Wormwood capillary (Artemisia capillaris) using inter simple sequence repeat (ISSR) in Pahang region, Malaysia. Plant Omics, 2: 127.


32. Stojanović-Radić, Z., Čomić, L., Radulović, N., Blagojević, P., Denić, M., Miltojević, A., Rajković, J. and Mihajilov-Krstev, T. 2012. Antistaphylococcal activity of Inula helenium root essential oil: eudesmane sesquiterpene lactones induce cell membrane damage. European journal of clinical microbiology & infectious diseases. 31: 1015-1025.


33. Wong, K.L., Wong, R.N.S., Zhang, L., Liu, W.K., Ng, T.B., Shaw, P.C., Kwok, P.C.L., Lai, Y.M., Zhang, Z.J. and Zhang, Y. 2014. Bioactive proteins and peptides isolated from Chinese medicines with pharmaceutical potential. Chinese medicine. 9: 19.


34. Wu, Z., Skjelvåg, A. and Baadshaug, O. 2004. Quantification of photoperiodic effects on growth of Phleum pratense. Annals of botany. 94: 535-543.


35. Zlatić, N., Jakovljević, D. and Stanković, M. 2019. Temporal, plant part, and interpopulation variability of secondary metabolites and antioxidant activity of Inula helenium Plants, 8: 179.


36. Zhou, J., Xie, G. and Yan, X. 2011. Encyclopedia of traditional Chinese medicines. Isolat Compound AB. 1: