Investigation and comparison of constituent EGCG of tea plant (Camellia sinensis (L.) Kuntze) harvested from the western region of Mazandaran province with callus obtained from tea plant tissue culture by novel method Molecularly Imprinted Polymer (MIP)
Subject Areas : PhytochemistryAzarm Movahedi 1 , Mazyar Ahmadi Golsefidi 2 , Mehdi Alizadeh 3
1 - Department of chemistry, Faculty of sciences, Gorgan branch, Islamic Azad University, Gorgan, Iran
2 - Department of chemistry, Faculty of sciences, Gorgan branch, Islamic Azad University, Gorgan, Iran.
3 - Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran
Keywords: Callus, tissue culture, Camellia sinensis L, EGCG, MIP,
Abstract :
Due to the importance of constituent Epigallocatechin gallate (EGCG), in the tea plant in use of pharmaceutical and food industries, the amount of EGCG in young leaf, old leaf and callus extract was compared and measured. EGCG in callus extract was isolated and measured by Molecularly Imprinted Polymer (MIP) technique in 1397 in Giah Essence Phytopharm Company. In order to callus induction, callus of tea plant which was harvested from the western region of Mazandaran province cultured in WPM, SH, MS media with different amounts of plant hormones. Leaves and callus extracted with 70% methanol by maceration method. For optimization of extraction and purification, various quantities of functional multiwalled-CNTs 1-10 mg used in production of CNTs-MIP and the performance investigated. EGCG of callus extract extracted with CNTs-MIP and its amount determined with HPLC. The highest volume of callus in WPM medium ,and the highest amount of EGCG in SH medium observed with optimized concentrations of hormones BA(2 mg/l), 2,4_D(0.5 mg/l). The highest content of EGCG was extracted by adding 8 mg of MWCNTs in the polymer structure. EGCG in young and old leaves and callus were 37.34, 7.19 and 0.13 mg/g of dried weight, respectively. By CNTs-MIP, the EGCG content in callus was obtained 0.91 mg/g. Despite the trace amount of EGCG in callus, the amount of this substance can be measured with a concentration coefficient of 8 times by CNTs-MIP, demonstrated the efficiency of this technique.
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