Effects of supercritical fluid and distillation extraction methods on the yield, antioxidant and antifungal activities of Artemisia persica Boiss essential oil

Subject Areas : Medicinal Plants

Parvin Ramak ¹ , Vahid Karimian ² , Asghar Sepahvand ³

1 - Lorestan Agricultural and Natural Resources Research and Education Center
2 - Young Researchers and Elite Club, Yasooj Branch, Islamic Azad University
3 - Razi Herbal Medicines Research Center , Lorestan University of Medical Sciences

Received: 2019-05-08 Accepted : 2019-08-24 Published : 2019-08-23

Keywords: Antifungal, Antioxidant, Artemisia persica, Supercritical extraction,

Abstract :

In this study, aerial parts of Artemisia persica in blooming satage were collected from Noor Abad Research Farm (Lorestan province, Iran) in the summer of 2017. Essential oils of plants were extracted by water distillation and supercritical fluid extraction (SFE) methods and were analyzed by using GC/MS device. Antifungal properties of essential oils were evaluated on Microsporum canis, Trichophyton rubrum, Epidermophyton floccosum, and Trichophyton mentagrophytes strains by broth microdilution method. Antioxidant activity of the essential oils was also evaluated by two free radical scavenging tests, viz. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Essential oil yields were 1.7% and 1.7-5.7% in water distillation and SFE methods, respectively. Major compounds identified in the SFE method were Davanone (29%), lacintafuran H (18.1%), C-artedoglacia oxide (8.5%), and neurodevnet (7.6%). Davanone (22%), lacintaphoran G (7.1%), and D-artedoglacia oxide (6%) were the predominant compounds detected in water distillation method. The essential oil extracted by water distillation had the highest antifungal effects on E. floccosum and T. montagrophytes strains with MFC and MIC of 32 µg/mL. Water extracted essential oil presented the highest free radical scavenging capacity in DPPH and ABTS tests with IC50 values of 20.32 and 14.23 μg/ml, respectively.

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