Evaluation of diversity in some phytochemical characteristics in leaf extract among some genotypes of Berberis species in North West of iran

Subject Areas : Medicinal Plants

naser gholizadeh ¹ , Bahman Hosseini ² , abolfazl Alirezalu ³

1 - Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran,
2 - Assocoite Prof., College of Agriculture, Urmia University of Urmia, Iran
3 - Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran,

Received: 2017-04-26 Accepted : 2017-06-14 Published : 2017-08-23

Keywords: Phenol, Flavonoid, Antioxidant, Carotenoid, Azerbaijahan, Berberis genotype,

Abstract :

Abstract In this study the leaves of 28 genotypes of Berberis were collected in May 2016 from North West of Iran. The samples were extracted by ultrasonic technique, total phenol, flavonoid, chlorophyll a, b and carotenoid were measured by folin ciocalteu, aluminum chloride and Litchenthaler method, respectively. The antioxidant capacity were evaluated differently by using FRAP and DPPH methods. The results were showed that the highest and the lowest total phenol content, were observed respectively, in genotypes Z8 and Z19 (75.96 - 13.59 mg GAE / gram dry weight), respectively.  The highest and the lowest total flavonoid were respectively in genotypes Z20 and Z19 (14.99 - 2.10 mg / 100 grams of dry matter). The highest of chlorophyll a, b and carotenoid were observed in genotypes Z3, Z26, Z8 respectively (26.32 - 5.37 and 18.86 mg dry matter). The highest antioxidant activity in DPPH was observed in Z6 genotype (92.30%) and in FRAP method with 4.14 µmol of iron per gram of dry matter was reported in Z8 genotype, respectively. According to these results it was found that the antioxidant activity correlated with total phenol and flavonoid contents and can be used these genotypes as a good natural  antioxidant in pharmaceutical industry's.

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