The assessment of total phenol, flavonoid, resveratrol content, and antioxidant capacity of Vitis vinife L., Pistacia vera L., Sambucus nigra L. and Ilex spinigera Loes

Subject Areas : Biochemistry

ruhollah dastoor ¹ , Davood Bakhshi ² , Alireza Aliakbar ³

1 - Department of Horticultural Science, University Campus2, University of Guilan, Rasht, Iran
2 - 2Department of Horticultural Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 - Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran

Received: 2017-02-24 Accepted : 2017-06-25 Published : 2017-08-23

Keywords: Phenol, Antioxidant, medicinal plants, Flavonoids, Resveratrol and Liquid Chromatography,

Abstract :

Resveratrol is one of the most polyphenol with antioxidant capacity which has ability to inhibit or retard a wide variety of disease. Vitis vinife L., Pistacia vera L., Sambucus nigra L. and Ilex spinigera Loes. , which are abundant resveratrol sources in Iran. Therefore the fruits of these plants in ripening stage were collected in 2015 from different regions of Iran  and were extracted by maceration method. The total phenol, flavonoid, resveratrol content and antioxidant capacity were measured by using Folin-Ciocalteu, aluminum chloride colorimetric assay, high performance liquid chromatography procedure (HPLC) and DPPH radical scavenging, respectively. The results were showed that  the highest of total phenol and antioxidant capacity were observed  in Sambucus  nigra  and Ilex spinigera, while the  Vitis vinifera had the richest source of resveratrol (0/04±0.003 mg/g DW) and flavonoid, respectively. So in conclusion all of these plants could be a good natural antioxidant  with the  high resveratrol content in pharmaceutical industry

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