Effects of jasmonic acid and titanium dioxide nanoparticles on process of changes of phytochemical and antioxidant in genotypes of Satureja hortensis L.
Subject Areas : PhytochemistryAzar Davari 1 , Mamoud Solouki 2 , Bahman Fazeli-Nasab 3
1 - M.Sc. of Plant Breeding, Graduated from University of Zabol, Zabol, Iran
2 - 2Associate Professor, Dept. of Breeding and Biotechnology, University of Zabol, Zabol, Iran
3 - 3Lecturer, Research Dept. of Agronomy and Plant Breeding, Agricultural Research
Institute, University of Zabol, Zabol, Iran
Keywords: Spraying, antioxidants, Jasmonic acid, Satureja hortensis L, Titanium dioxide nanoparticles,
Abstract :
In this research to evaluate the effect of sprying of titanium dioxide nanoparticles (0, 30, 60 and 90 mg/l) and jasmonic acid (0, 50, 100 and 150 mM) on enzymatic and non-enzymatic antioxidants As well as physiological traits 5 genotype of Satureja hortensis L. factorial experiment in a completely randomized design with three replications. Treatments were conducted at four leaf stage on the aerial parts of the plants and then were harvested 48 hours after spraying. At first were extracted from shoots and then all traits; enzymatic and non-enzymatic antioxidants and physiological traits were measured by using a spectrophotometer. Analysis of variance showed that the interaction of spraying different concentrations of titanium dioxide nanoparticles and jasmonic acid, catalase, peroxidase, polyphenol oxidase, ascorbate peroxidase, total protein, flavonoids, anthocyanins and total phenolics were significant at the 1% level. The highest catalase (0.0536 mg per gram of fresh weight) at doses of 30 mg per liter in Tabriz plant genotype nanoparticles of titanium dioxide and 150 mMjasmonic acid per liter;Most of ascorbate peroxidase (0.0514 mg per gram of fresh weight) in plant genotypes in concentrations of 60 mg per liter Ardestān nanoparticles of titanium dioxide and 50 mMjasmonic acid;Most peroxidase activity (0.000494 mg per gram of fresh weight) at a concentration of 60 mg per liter in genotype Savory Ardestān nanoparticles of titanium dioxide and 150 mMjasmonic acid; The highest polyphenol oxidase (0.0628 mg per gram of fresh weight) in Tabriz genotypes in treatments of 30 mg of nanoparticles of titanium dioxide and zero (control) M jasmonic acid was obtained.Savory population most effective physiological traits, Savory Rey, the antioxidant enzyme, Savory Ardestan and Tabriz and the non-enzymatic antioxidants (anthocyanins, phenols and flavonoids) Savory Mashhad. The results of this study showed that nano-titanium and jasmonic caused time and non-enzymatic antioxidant and physiological Savory is the most effective hormone levels nanoparticles of titanium, 30 ppm and jasmonic acid was 50 micro molar.
Ali, M.B., Yu, K.W., Hahn, E.J. and Paek, K.Y. 2006. Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors. Plant cell reports 25(6): 613-620.
2.Alizadeh, N., Majd, A., Mahmoudzadeh, H. and Jonubi, P. 2015. The effects of zinc oxide nanoparticles on some biochemical characteristics of safflower(Carthamus tinctorius L.). 2th National Conference of medicinal herbs, traditional medicine and organic farming 13-15.
3.Ashrafi, M., ghasemi Pirbalouti, A., Rahimmalek, M. and Hamedi, B. 2012. Effect of foliar application of Jasmonic Acid (JA) on essential oil yield and its compositions of Thymus daenensis Celak. Journal of Herbal Drugs 3(2): 75-80.
4.Bari, R. and Jones, J.D. 2009. Role of plant hormones in plant defence responses. Plant molecular biology 69(4): 473-488.
5.Beers, R.F. and Sizer, I.W. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol chem 195(1): 133-140.
6.Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72(1-2): 248-254.
7.Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis 10(3).
8.Cunningham, B.W., Orbegoso, C.M., Dmitriev, A.E., Hallab, N.J., Sefter, J.C. and McAfee, P.C. 2002. The effect of titanium particulate on development and maintenance of a posterolateral spinal arthrodesis: an in vivo rabbit model. Spine 27(18): 1971-1981.
9.Eckhardt, U., Grimm, B. and Hörtensteiner, S. 2004. Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant molecular biology 56(1): 1-14.
10.Ehsanpour, A.A. and Nejati, Z. 2012. The effect of nanosilver on chlorophyll, gibberellic acid electrophoretic pattern of proteins in vitro culture of plant potato variety Desiree White. Journal of Applied biology 1(1): 13-26. 10.22051/jab. 2014.1123
11.García, A., Delgado, L., Torà, J.A., Casals, E., González, E., Puntes, V., Font, X., Carrera, J. and Sánchez, A. 2012. Effect of cerium dioxide, titanium dioxide, silver, and gold nanoparticles on the activity of microbial communities intended in wastewater treatment. Journal of hazardous materials 19964-72.
12.Ghanati, F., Bakhtiairan, S. and Abdolmaleki, P. 2010. The Effects of methyl jasmonate on secondary metabolites of Calendula officinalis. Journal of Science and Biotechnology 122-32.
13.Ghasemi, A. 2009. Medicinal and Aromatic Plants. Ilamic Azad University press of ShahreKord541.
14.Ghasemi Pirbaloti, A., Mousavi Harris, S.A., Tirgir, F. and B, H. 2012. The effect of jasmonic acid and salicylic acid on the phenolic compounds and flavonoids in extract of Calendula officinalis. Journal of Herbal medicines 3175-180.
15.Hu, R., Gong, X., Duan, Y., Li, N., Che, Y., Cui, Y., Zhou, M., Liu, C., Wang, H. and Hong, F. 2010. Neurotoxicological effects and the impairment of spatial recognition memory in mice caused by exposure to TiO 2 nanoparticles. Biomaterials 31(31): 8043-8050.
16.Jin, C.Y., Zhu, B.S., Wang, X.F. and Lu, Q.H. 2008. Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells. Chemical research in toxicology, 21(9): 1871-1877.
17. Kang, S.J., Kim, B.M., Lee, Y.J. and Chung, H.W. 2008. Titanium dioxide nanoparticles trigger p53‐mediated damage response in peripheral blood lymphocytes. Environmental and molecular mutagenesis, 49(5): 399-405.
18.Kar, M. and Mishra, D. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant physiology, 57(2): 315-319.
19.Kim, H.-J., Chen, F., Wang, X. and Rajapakse, N.C. 2005. Effect of chitosan on the biological properties of sweet basil (Ocimum basilicum L.). Journal of agricultural and food chemistry, 53(9): 3696-3701.
20.Koo, A.J. and Howe, G.A. 2009. The wound hormone jasmonate. Phytochemistry, 70(13): 1571-1580.
21.Kovac, M. and Ravnikar, M. 1994. The effect of jasmonic acid on the photosynthetic pigments of potato plants grown in vitro. Plant Science, 103(1): 11-17.
22. Lee, S., Kim, S., Kim, S. and Lee, I. 2013. Assessment of phytotoxicity of ZnO NPs on a medicinal plant, Fagopyrum esculentum. Environmental Science and Pollution Research, 20(2): 848-854.
23.Li, N., Duan, Y., Hong, M., Zheng, L., Fei, M., Zhao, X., Wang, J., Cui, Y., Liu, H. and Cai, J. 2010. Spleen injury and apoptotic pathway in mice caused by titanium dioxide nanoparticules. Toxicology letters, 195(2): 161-168.
24.Liu, S., Xu, L., Zhang, T., Ren, G. and Yang, Z. 2010. Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells. Toxicology, 267(1): 172-177.
25.Long, T.C., Tajuba, J., Sama, P., Saleh, N., Swartz, C., Parker, J., Hester, S., Lowry, G.V. and Veronesi, B. 2007. Nanosize titanium dioxide stimulates reactive oxygen species in brain microglia and damages neurons in vitro. Environmental Health Perspectives, 1631-1637.
26.Martin, D., Tholl, D., Gershenzon, J. and Bohlmann, J. 2002. Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant physiology, 129(3): 1003-1018.
27.Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food chemistry, 91(3): 571-577.
28.Mohammadi, M. and Kazemi, H. 2002. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Science, 162(4): 491-498.
29.Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and cell physiology, 22(5): 867-880.
30.Nicoli, M.C., Elizalde, B.E., Pitotti, A. and Lerici, C.R. 1991. Effect of sugars and maillard reaction products on polyphenol oxidase and peroxidase activity in food. Journal of Food Biochemistry, 15(3): 169-184.
31.Novak, J., Bahoo, L., Mitteregger, U. and Franz, C. 2006. Composition of individual essential oil glands of savory (Satureja hortensis L., Lamiaceae) from Syria. Flavour and Fragrance Journal, 21(4): 731-734.
32.Ochekpe, N.A., Olorunfemi, P.O. and Ngwuluka, N.C. 2009. Nanotechnology and drug delivery part 1: background and applications. Tropical journal of pharmaceutical research, 8(3): 265-274.
33.Pandey, N., Gupta, B. and Pathak, G. 2012. Antioxidant responses of pea genotypes to zinc deficiency. Russian Journal of Plant Physiology, 59(2): 198-205.
34.Parivar, K., Badiei, A. and Zolfaghari Barogh, S. 2014. Study of nano-titanium dioxide effects on the fore limb bud development of NMRI strain mouse embryo in vitro. New Cellular and Molecular Biotechnology Journal, 4(13): 43-49.
35.Peyvandi, M., Mirza, M. and Kamali Jamakani, Z. 2011. The Effect of Nano Fe Chelate and Fe Chelate on the Growth and Activity of some Antioxidant. New Cellularand Molecular Biotechnology Journal 2(5): 25-32.
36.Rahman, Q., Lohani, M., Dopp, E., Pemsel, H., Jonas, L., Weiss, D.G. and Schiffmann, D. 2002. Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environmental Health Perspectives, 110(8): 797.
37.Rubio, V., Bustos, R., Irigoyen, M.L., Cardona-López, X., Rojas-Triana, M. and Paz-Ares, J. 2009. Plant hormones and nutrient signaling. Plant molecular biology, 69(4): 361-373.
38.Salari, F.A.M., Hakimeh 2013. The effect of jasmonic acid on the terpenoid compounds in Cannabis sativa. Journal of plant process and function, 1(2): 51-60.
39.Shabani, L. and Ehsan, P.A.A. 2009. Induction of antioxidant enzymes, phenolics and flavonoids in vitro culture of licorice (Glycyrrhiza glabra) using methyl jasmonate and salicylic acid. Iranian Journal of Biology, 22(4): 691-703.
40.Shahsavari, A. and Maaboudi, S. 2012. The study of observed species of Anchusa L. genus (Boraginaceae) in ruderal and segetal environments of Hamedan city (west of Iran). Journal of Plant Research (Iranian Journal of Biology), 27(4): 674-680.
41. Sinha, S. and Saxena, R. 2006. Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere 62(8): 1340-1350.
42.Soltani, M., Moaafi, P. and Nouri, H. 2004. The effect of foliar application of titanium dioxide nanoparticles on yield and activities of antioxidant enzymes in lentil (Lens culiaris Medik.). Iranian Journal of preceding studies of plant physiological, 9(1): 78-88.
43.Wang, S.Y., Bowman, L. and Ding, M. 2008. Methyl jasmonate enhances antioxidant activity and flavonoid content in blackberries (Rubus sp.) and promotes antiproliferation of human cancer cells. Food chemistry, 107(3): 1261-1269.
44.Wong, C.-C., Li, H.-B., Cheng, K.-W. and Chen, F. 2006. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food chemistry, 97(4): 705-711.
