The effects of NPK, chemical and manure fertilizers investigation on the phenolic yield and essential oil components in Thymus daenensis L.

Subject Areas : Medicinal Plants

Safaei Leily ¹ , Ebrahim Sharifi ashoorabadi ² , Davood Afiuni ³

1 - Research Division of Natural Resources, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran.
2 - Iranian Research Institute of Forest and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
3 - Horticulture Crops Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran.

Received: 2016-12-05 Accepted : 2017-02-28 Published : 2017-05-22

Keywords: Essential oil, thymol, Combined fertilizer, Phenole, Thymus daenensis L,

Abstract :

In order to study the effect of chemical fertilizer, manure and their combination on phytochemistry of Thymus daenensis L., this research was coducted using a randomized complete block design with split plot in time layout including 12 treatments and 3 replications. Treatments were 3 rates of N. P. K chemical fertilizers, 4 rates of manure fertilizer, combination of chemical fertilizer and manure in four rates and control (no fertilizer application). The essential oil was extracted by hydro distillation method by using a Clevenger-type apparatus and were analyzed by  GC and GC-MS. The total phenols were measurred by spectrophotometry. The results were showed that the highest phenolic content (57.17 kg/ha), essential oil yeild (3.88%) and thymol (86.68%) were obtained by application of 40 kg N, 32 kg P and 32 kg K combined with 25 ton/ha manure , but the highest carvacrol percentage (10.11%) obtained by application of 25 ton/ha manure. In all treatments the thymol, carvacrol and e-caryophyllene were the main essential oil components and different treatments had no effect on essential oil composition. There is a negative significant correlation was observed between thymol with carvacrol and e-caryophyllene. This  findings may provide a hopeful perspective to replacing chemical fertilizers by organic fertilizers. In conclusion, the  quantitative and qualitative yield in combined applicaion of chemical and organic fertilizers may be superior than chemical or organic systems alone and it can be concluded that this combined system is adapted with sustainable agriculture.

References:

References

1.Abdelaziz, M., Pokluda, R. and Abdelwahab, M. 2007. Influence of compost, micriorganism and NPK Fertilizers upon growth, chemical composition and essential oil production of Rosmarinus officinalis L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca., 35, (1): 86-90.

2.Akbarinia, A. and Mirza, M. 2008. Identification of thyme drug yield under cultivated condition. Iranian Journal of Ghazvin Medical Sciences University, 12(3): 59- 62.

3.Akbarinia, A., Ghalavand, A., Sefidkon, F., Rezaii, M.B. and Sharifi Ashoorabadi, A. 2003. Study the effects of NPK, manure and combination of chemical and manure fertilizers on yield and essential oil yield of Carum copticum. Iranian Journal of Pajoohesh and sazandegi, 61: 32- 41.

4.Akbarinia, A., Sharifi Ashoorabadi, A. and Mirza, M. 2010. Study on drug yield and essential oil content of Thymus daenensis Celak. under cultivated condition. Iranian Journal of Medicinal and Aromatic Plants, 26(2): 205-212.

5.Ali, M.Y.M. 2002. Physiological studies on Foeniculum vulgare Mill plants under Sinai conditions. M. Sc. Thesis, Faculty of Agriculture, Cairo University.

6.Al-Qadasi, A.S.S. 2004. Effect of biofertilization on Ocimum basilicum L. plants. M.Sc. Thesis, Faculty of Agriculture, Cairo University, Egypt.

7.Attia, M., Elham, H.M. and Abdel Azeem, H. 2004. Effect of biofertilization with some strains of bacteria and chemical fertilization on Mentha viridis L. cultivated in Maruit location. 9th Conference of Agricultural Develop. Researches, Ain Shams Univ.,Cairo Egypt. Annals of Agriculture Sci., Sp. 2: 431-442.

8.Ayanoglu, F., Mert, A. and Kirici, S. 2002. The effect of different nitrogen doses on Artemisia annua L. Journal of Herbs, Spices and Medicinal plants, 9: 399-404.

9.Baranauskiene, R., Venskutonis, P.R., Viskelis, P.and Dambrauskiene, E.  2003. Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). Journal of Agriculture and Food Chemistry, 51(26): 7751-8.

10.Barazandeh, M. and Bagherzadeh, K. 2007. Study of T. daenensis drug yield from 4 regions of Esfahan. Iranian Journal of Medicinal Plants, 6(3): 15- 19.

11.Bashon, Y., Levanony, H. and Whitmoyer, R. 1991. Root surface colonization of non-cereal crop plants by pleomorphic Azospirillum brasilense Cd. Journal of General Microbiology, 137: 187- 196.

12.British Pharmacopoeia, 1988, London, United Kingdom, pp: 2, A137 – A138.

13. Ceylan, A., Bayram, E. and Ozay, N., 1995. The effects of N-fertilizer on the yeild and quality of Thymus vulgaris L. in ecological conditions of Bornova- Izmir. Hort. Absts. 65: abst: 5368.

14.Dambrauskiene, E. and Venskutonis, R. 1999, Effect of nitrogen fertilizers on the yield of first year thyme and its quality, Horticulture and Vegetable Growing, 18(3): 107-112.

15. Dzida, K., 2007. Influence of varied nitrogen- potassium fertilizationon yield, essential oil content and mineral composition in darden thyme herb (Thymus vulgaris L.). Herba Polonica, 53(3): 146- 150.

16.Ebrahim, M.M.H. 2005. Effect of some agricultural treatments on roselle plant in the new cultivated soil. M. Sc. Thesis, Faculty of Agriculture, Zagazig University, Egypt.

17.Edris, A.E, Shalaby, A. and Fadel, H.M. 2003. Effect of organic agriculture practices on the volatile aroma components of some essential oil plants growing in Egypt. II. Sweet marjoram (Origanum marijorana L.) essential oil. Flavour and Fragrance Journal. 18: 345-351.

18.Hanafy Ahmed, A.H., Nesiem, M.R.A., Henwedy, A.M. and Sallam, H.E.E. 2002. Effect of organic manures, biofertilizers and NPK mineral fertilizers on growth, yield, chemical composition and nitrate accumulation of eweet peper plants. Proceeding of the 2^nd International Congress on Recent Technologie in Agriculture, Faculty of Agriculture, Cario University. 28-30 October, 4:932- 955.

19.Hendawy, S.F., Azza, A.E., Aziz, E. and Omer, E.A. 2010. Productivity and oil quality of Thymus vulgaris L. under organic fertilization conditions, Ozean Journal of Applied Sciences, 3(2): 203-216.

20.Jacoub, R.W. 1999. Effect of some organic and in-organic fertilizers on growth, oil yield and chemical composition of Ocimum basilicum L. and Thymus vulgaris L. plants, PhD. Thesis, Faculty of Agricultyre, Cairo University, Egypt.

21.Malakooti, M.J. 1996. Sustainable agriculture and increased performance with optimizing fertilizer in Iran. Research, education, promotion of agriculture publication. 297 pp.

22.Nikolova, A., Kozhuharova, K., Zheljazkov, V.D. and Craker, L.E. 1999. Mineral nutrition of chamomile (Chamomilla recutita L.). Acta Hort. 502: 203-208.

23.Nikavar, B., Mojab, F. and Doolatabadi, R. 2004. Study of component of Thymus daenensis flowering branches. Iranian Journal of Medicinal Plants, 4(13): 45-49.

24.Omidbaigi, R. and Arjmandi, A. 2002. Effects of NP supply on growth, development, yields and active substances of garden thyme (Thymus vulgaris L.). Acta Horticulture, 576: 263- 265.

25.Omidbaigi, R. and Rezaei Nejad, A. 2000. The influence of nitrogen fertilizer and harvest time on the productivity of Thymus vulgaris. International Journal of Horticulture Science, 6: 43-46.

26.Paschalina, S., Koutsos, T.V. and Katsiotis, S.T. 2006. Study of nitrogen fertilizers rate on fennel cultivars for essential oils yield and composition. Journal of Vegetable science, 12: 85- 93.

27.Rechinger, K.H. and Hedge, I.C. 1986 .Umbelliferae. In: Rechinger. K. H. Flora Iranica. Graz: Akademische Druck– u Verlagsanstatlt, Vol 162.

28.Safaii, L., Sharifi Ashoorabadi, A., Afiuni, D., Davazdah-Emami, S, and Shoaii, A.A., 2014. The effect of different nutrition systems on aerial parts and essential oil yield of Thymus daenensis. Iranian Journal of Medicinal and Aromatic Plants Research, 30(5): 702- 713.

29.Sangwan, N.S., Farooqi, A.H.A., Shabih, F. and Sangwan, R.S., 2001. Regulation of essential oil production in plants. Plant growth regulation, 34: 3-21.

30.Shalaby, S. and Razin, S.H., 1992. Dense cultivation and fertilization for higher yield of Thyme (Thymus vulgaris L.). Journal of Agronomy and Crop Science, 168(4): 243–248.

31.Sharafzadeh, Sh., khoshkhooi, M. and Javidnia, M., 2008. Effects of nutrients on growth and active substances of Thyme (Thymus vulgaris L.). Iranian Journal of Horticultural Science and Technology, 9(4): 261- 274.

32.Sharifi Ashoorabadi, A., matin, A. and Lebaschi, M., 2003. Plant growth indices of fennel Under different conditions of soil fertility. Iranian Journal of Medicinal and Aromatic Plants Research, 19(2): 157- 182.

33.Taherian, A., Rashidipoor, A., Vafaii, A., jarahif, M., Miladi Gorjii, H., Emami Abargooii, M. and Sadeghi, H., 2004. Assessment the effects of hydroalcoholic extract of Thymus vulgaris on acute pain in hot plate and tail flick in mice. Iranian Journal of koomesh, 5 (3):179-185.