Phytochemical response of physalis alkekengi L. to foliar application of amino acids.
Subject Areas :
Medicinal Plants
Sirous
Saremi
1
(phD student, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran)
Manouchehr
Gholipoor
2
(Associate professor, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran)
Hamid
Abbasdokht
3
(Associate professor, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran)
Hassanali
Naghdi Badi
4
(Associate professor, Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran)
Ali
Mehrafarin
5
(Assistant professor, Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran)
Hamid reza
Asghari
6
(Associate professor, Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran)
Received: 2020-11-26
Accepted : 2021-04-10
Published : 2021-08-23
Keywords:
Ascorbic acid,
Carotenoid,
amino acid,
alkaloid,
phenol,
physalis alkekengi L,
Abstract :
Biostimulants originate from natural and biological sources and in addition to improving plant growth and soil yield, they can protect plants from environmental stresses and stimulate the synthesis of secondary metabolites involved in plant defense mechanism. In this study, to study the effect of foliar application of various amino acids on the biochemical responses of Physalis alkekengi L. an experiment in the form of randomized complete block design with 3 replications in the farm of research Institute of Medicinal Plants of jahad University in Karaj was conducted in the cropping year of 2018-19.Treatments at five levels of amino acid foliar application including {control (A0), tryptophan (1 mg/L) (A1), glycine (1 mg/L) (A2), tyrosine (1 mg/L) (A3) and arginine (1 mg/L) (A4) were administered. Measured variables included: carotenoids, anthocyanins, proline, phenol, beta-carotene, lycopene, ascorbic acid, and alkaloids. The dimensions of the plots were 3×3 m, the distance between each plot was considered to be one meter. Planting operations were done manually in May 2017. The distance between the plants on the culture lines was 25 cm and the distance between the culture lines was 60 cm. Foliar spraying on plants began before flowering. At the time of sampling, three replicates were taken from each treatment and three samples were taken from each replication. Chlorophyll and carotenoids by Lichtenthaler method, anthocyanin by Mita method, proline by Bates method, phenol by Meda method, beta-carotene by Amarowicz method, lycopene by Montesano method, ascorbic acid by titration method, and alkaloid by Iranian Herbal Pharmacopeia method, were measured. The results showed that foliar application of amino acids had a significant effect (p ≤ 0.01) on all evaluated traits and the highest amounts of carotenoids (12.83 mg/gfw), beta-carotene (0.035 mg/gfw), and lycopene (0.017 mg/gfw) from arginine foliar application, highest ascorbic acid (24.2 mg/gfw) and alkaloids (42.25%) from tryptophan foliar application, highest proline (1.4 mg/gfw) Glycine and the highest levels of anthocyanin (12.25 mg/gdw) and phenol (32.44 mg/gfw) were obtained from the control treatment (no foliar application). In general, the results showed that foliar application of amino acids as a biological stimulant had a positive effect on improving the quality characteristics of the Physalis alkekengi L and increased the production of biochemical compounds of the plant.
References:
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Abaspour Esfaden, M., Kallaterjari, S. and Fatehi, F. 2019. The effect of salicylic acid and L-arginine on morpho-physiological properties and leaf nutrients of Catharanthus roseus under Drought Stress. Journal of Horticultural Science, 33(3): 417-432.
Abdossi, V. and Danaee, E. 2019. Effects of some amino acids and organic acids on enzymatic activity and longevity of Dianthus caryophyllus tessino at pre-harvest stage. Journal of Ornamental Plants, 9(2): 93-104.
Amarowicz, R., Pegg, R., Rahimi-Moghaddam, P., Barl, B. and Weil, J. 2004. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food chemistry, 84(4): 551-562.
Amin, A., Gharib, F.A., El-Awadi M. and Rashad, E.S.M. 2011. Physiological response of onion plants to foliar application of putrescine and glutamine. Scientia horticulturae, 129(3): 353-360.
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Gendy, A.S. and Nosir, W.S. 2016. Improving productivity and chemical constituents of Roselle plant (Hibiscus sabdariffa) as affected by phenylalanine, L-tryptophan and peptone acids foliar application. Middle East Journal of Agriculture, 5(4): 701-708.
Hakimi, L., Naiebzadeh, M. and Khaligi, A. 2019. Investigating the effect of glycine betaine and humi-forthi on morpho-physiological and biochemical properties Pelargonium graveolens under water stress. Journal of Plant Production Research, 26(3): 37-56.
Hojagan, M.P., Arooie, H., Tabatabaei S.J. and Neamati, S.H. 2017. Effect of amino acids foliar spraying on growth and physiological indices of tomato under salt stress conditions. Agroecology Journal, 13(3): 41-50.
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Miri, S.M., Ahmadi, S., and Moradi, P. 2015. Influence of salicylic acid and citric acid on the growth, biochemical characteristics and essential oil content of thyme (Thymus vulgaris). Journal of medicinal plants and by products. 4(2):141-146.
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Montesano, D., Fallarino, F., Cossignani, L., Bosi, A., Simonetti, M.S., Puccetti, P. and Damiani, P. 2008. Innovative extraction procedure for obtaining high pure lycopene from tomato. European Food Research and Technology, 226(3): 327.
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Pharmacopeia, I.H. 2002. Isfand 1381, Project.
Portu, J., López-Alfaro, I., Gómez-Alonso, S., López, R. and Garde-Cerdán, T. 2015. Changes on grape phenolic composition induced by grapevine foliar applications of phenylalanine and urea. Food chemistry, 180: 171-180.
Poursoltan Hojagan, M., Arouiee, H., Tabatabaei, S.J. and Nemati, S.H. 2017. Priming impacts on seed quality of tomato (Lycopersicon esculentum) under salinity condition. International Journal of Agronomy and Agricultural Research, 11(3): 104-109.
Radkowski, A. 2018. Influence of foliar fertilization with amino acid preparations on morphological traits and seed yield of timothy." Plant, Soil and Environment, 64(5): 209-213.
Refaat, A. and Naguib, N. 1998. Peppermint yield and oil quality as affected by application of some amino acids. Bulletin-Faculty of Agriculture University of Cario, 49: 89-98.
Rezaie Alulu, A., Kheiry, A., Sanikhani, M., and Arghavani, M. 2019. Effect of salicylic acid and glycine betaine foliar application on morpho-physiological characteristics of carla (Momordica charantia) under water deficit stress. Journal of Agricultural Science and Sustainable Production, 29(1): 223-235.
Sanikhani, M., Akbari, A., and Kheiry, A. 2020. Effect of phenylalanine and tryptophan on morphological and physiological characteristics in colocynth (Citrullus colocynthis). Journal of Plant Process and Function, 9 (35): 317-328.
Shehata, S., Abdel-Azem, H.S., Abou El-Yazied, A., and El-Gizawy, A. 2011. Effect of foliar spraying with amino acids and seaweed extract on growth chemical constitutes, yield and its quality of celeriac plant. European Journal of Scientific Research, 58(2): 257-265.
Sheokand, S., Kumari, A., and Sawhney, V. 2008. Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants. Physiology and Molecular Biology of Plants, 14(4): 355-362.
Shokouh, A.R., Mehrafarin, A., Abdossi, V. and Naghdi Badi, H. 2018. Morpho-physiological and biochemical responses of bladder cherry (Physalis alkekengi) induced by multienzymatic biostimulant, IBA, and citric acid. Folia Horticulturae, 30(1): 79-92.
Siahmansour, S., Ehtesham-Nia, A., and Rezaeinejad, A. 2020. Effect of salicylic acid foliar application on Morpho-physiological and biochemical traits of Goldenberry (Physalis peruviana) under salinity stress condition. Journal of Plant Production Research, 27(1): pp.165-178.
Taiz, L., and Zeiger, E. 1991. Plant physiology. Benjamin/Cummings series in the life sciences (USA).
Tajik, N., and Danaee, E. 2016. Study the effect of spraying pre-harvest glotamin, arginine and phenylalanine on some physicochemical and enzymatic traits and longevity gerbera jamesonii flower cv. Sorbet Cellular and Molecular Plant Biology Journal, 8(3): 4.
Tang, W., and Newton, R.J. 2005. Polyamines reduce salt-induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in virginia pine. Plant Growth Regulation, 46(1): 31-43.
Wahba, H., Motawe, H., and Ibrahim, A. 2015. Growth and chemical composition of Urtica pilulifera plant as influenced by foliar application of some amino acids. Journal of Materials and Environment Science, 6(2): 499-506.
Yuan, S. and Lin, H.H. 2008. Minireview: role of salicylic acid in plant abiotic stress. Zeitschrift für Naturforschung C, 63(5-6): 313-320.
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Abd El-Wahed, M., Karima, M. and Gamal-El-Din, M. 2005. Effect of putrescine and atonik on growth and some biochemical constituents as well as essential oil composition of chamomile plant (Chamomilla recutita, Rausch.). Journal of Agriculture Science Mansoura University, 30(2): 869-882.
Abaspour Esfaden, M., Kallaterjari, S. and Fatehi, F. 2019. The effect of salicylic acid and L-arginine on morpho-physiological properties and leaf nutrients of Catharanthus roseus under Drought Stress. Journal of Horticultural Science, 33(3): 417-432.
Abdossi, V. and Danaee, E. 2019. Effects of some amino acids and organic acids on enzymatic activity and longevity of Dianthus caryophyllus tessino at pre-harvest stage. Journal of Ornamental Plants, 9(2): 93-104.
Amarowicz, R., Pegg, R., Rahimi-Moghaddam, P., Barl, B. and Weil, J. 2004. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food chemistry, 84(4): 551-562.
Amin, A., Gharib, F.A., El-Awadi M. and Rashad, E.S.M. 2011. Physiological response of onion plants to foliar application of putrescine and glutamine. Scientia horticulturae, 129(3): 353-360.
Aminifard, M., Gholami, M., Bayat, H. and Moradinezhad, F. 2020. Effect of fulvic acid and amino acid application on physiological characteristics, growth and yield of coriander (Coriandrum sativum) as a medicinalplant. Journal of Agroecology, 12(3): 373-388.
Bates, L.S., Waldren R.P. and Teare, I. 1973. Rapid determination of free proline for water-stress studies. Plant and soil, 39(1): 205-207.
Calvo, P., Nelson, L. and Kloepper, J.W. 2014. Agricultural uses of plant biostimulants. Plant and soil, 383(1-2): 3-41.
Horwitz, W., Chichilo, P. and Reynolds, H. 1970. Official methods of analysis of the Association of Official Analytical Chemists Washington, DC.
Danaee, E. and Abdossi, V. 2019. Phytochemical and morphophysiological responses in basil (Ocimum basilicum) plant to application of polyamines. Journal of Medicinal Plants, 18 (69):125-133.
Davies, P.J. 2010. The plant hormones: their nature, occurrence, and functions. Plant hormones, Springer: 1-15.
El-Shabasi, M., Mohamed S. and Mahfouz, S. 2005. Effect of foliar spray with amino acids on growth, yield and chemical composition of garlic plants. The sixth Arabian Conference for Horticulture, Ismailia, Egypt.
Garcia, A., Madrid, R. Gimeno, V., Ortega, W.R., Nicolás, N. and Sánchez, F.G. 2011. The effects of amino acids fertilization incorporated to the nutrient solution on mineral composition and growth in tomato seedlings. Spanish Journal of Agricultural Research, (3): 852-861.
Gendy, A.S. and Nosir, W.S. 2016. Improving productivity and chemical constituents of Roselle plant (Hibiscus sabdariffa) as affected by phenylalanine, L-tryptophan and peptone acids foliar application. Middle East Journal of Agriculture, 5(4): 701-708.
Hakimi, L., Naiebzadeh, M. and Khaligi, A. 2019. Investigating the effect of glycine betaine and humi-forthi on morpho-physiological and biochemical properties Pelargonium graveolens under water stress. Journal of Plant Production Research, 26(3): 37-56.
Hojagan, M.P., Arooie, H., Tabatabaei S.J. and Neamati, S.H. 2017. Effect of amino acids foliar spraying on growth and physiological indices of tomato under salt stress conditions. Agroecology Journal, 13(3): 41-50.
Kahlel, A.-M.S. and Sultan, F.I. 2019. Response of four potato cultivars to soil application with organic and amino acid compounds." Research on Crops, 20(1): 101-108.
Kaya, C., Tuna, A.L., Ashraf, M. and Altunlu, H. 2007. Improved salt tolerance of melon (Cucumis melo) by the addition of proline and potassium nitrate. Environmental and Experimental Botany, 60(3): 397-403.
Kheiri, A. and Arghavani, M. 2019. Biofertilizers effects on quality and quantity characteristics of winter cherry (Physalis alkekengi) medicinal plant, 8(29): 273-286.
Liang, Z., Ma, Y., Xu, T., Cui, B., Liu, Y., Guo, Z., and Yang, D. 2013. Effects of abscisic acid, gibberellin, ethylene and their interactions on production of phenolic acids in Salvia miltiorrhiza Bunge hairy roots. PloS one, 8(9): e72806.
Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in enzymology, Elsevier, 148: 350-382.
Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. 2005. Determination of the total phenolic, flavonoid and proline contents in burkina fasan honey, as well as their radical scavenging activity. Food chemistry, 91(3): 571-577.
Miri, S.M., Ahmadi, S., and Moradi, P. 2015. Influence of salicylic acid and citric acid on the growth, biochemical characteristics and essential oil content of thyme (Thymus vulgaris). Journal of medicinal plants and by products. 4(2):141-146.
Mita, S., Murano, N., Akaike, M. and Nakamura, K. 1997. Mutants of arabidopsis thaliana with pleiotropic effects on the expression of the gene for β‐amylase and on the accumulation of anthocyanin that are inducible by sugars. The Plant Journal, 11(4): 841-851.
Montesano, D., Fallarino, F., Cossignani, L., Bosi, A., Simonetti, M.S., Puccetti, P. and Damiani, P. 2008. Innovative extraction procedure for obtaining high pure lycopene from tomato. European Food Research and Technology, 226(3): 327.
Nahed, G.A.A., Lobna, S.T., and Soad, M.I. 2009. Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus plants at Nubaria. Ozean Journal of Applied Science, 2(2): 169-179.
Pharmacopeia, I.H. 2002. Isfand 1381, Project.
Portu, J., López-Alfaro, I., Gómez-Alonso, S., López, R. and Garde-Cerdán, T. 2015. Changes on grape phenolic composition induced by grapevine foliar applications of phenylalanine and urea. Food chemistry, 180: 171-180.
Poursoltan Hojagan, M., Arouiee, H., Tabatabaei, S.J. and Nemati, S.H. 2017. Priming impacts on seed quality of tomato (Lycopersicon esculentum) under salinity condition. International Journal of Agronomy and Agricultural Research, 11(3): 104-109.
Radkowski, A. 2018. Influence of foliar fertilization with amino acid preparations on morphological traits and seed yield of timothy." Plant, Soil and Environment, 64(5): 209-213.
Refaat, A. and Naguib, N. 1998. Peppermint yield and oil quality as affected by application of some amino acids. Bulletin-Faculty of Agriculture University of Cario, 49: 89-98.
Rezaie Alulu, A., Kheiry, A., Sanikhani, M., and Arghavani, M. 2019. Effect of salicylic acid and glycine betaine foliar application on morpho-physiological characteristics of carla (Momordica charantia) under water deficit stress. Journal of Agricultural Science and Sustainable Production, 29(1): 223-235.
Sanikhani, M., Akbari, A., and Kheiry, A. 2020. Effect of phenylalanine and tryptophan on morphological and physiological characteristics in colocynth (Citrullus colocynthis). Journal of Plant Process and Function, 9 (35): 317-328.
Shehata, S., Abdel-Azem, H.S., Abou El-Yazied, A., and El-Gizawy, A. 2011. Effect of foliar spraying with amino acids and seaweed extract on growth chemical constitutes, yield and its quality of celeriac plant. European Journal of Scientific Research, 58(2): 257-265.
Sheokand, S., Kumari, A., and Sawhney, V. 2008. Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants. Physiology and Molecular Biology of Plants, 14(4): 355-362.
Shokouh, A.R., Mehrafarin, A., Abdossi, V. and Naghdi Badi, H. 2018. Morpho-physiological and biochemical responses of bladder cherry (Physalis alkekengi) induced by multienzymatic biostimulant, IBA, and citric acid. Folia Horticulturae, 30(1): 79-92.
Siahmansour, S., Ehtesham-Nia, A., and Rezaeinejad, A. 2020. Effect of salicylic acid foliar application on Morpho-physiological and biochemical traits of Goldenberry (Physalis peruviana) under salinity stress condition. Journal of Plant Production Research, 27(1): pp.165-178.
Taiz, L., and Zeiger, E. 1991. Plant physiology. Benjamin/Cummings series in the life sciences (USA).
Tajik, N., and Danaee, E. 2016. Study the effect of spraying pre-harvest glotamin, arginine and phenylalanine on some physicochemical and enzymatic traits and longevity gerbera jamesonii flower cv. Sorbet Cellular and Molecular Plant Biology Journal, 8(3): 4.
Tang, W., and Newton, R.J. 2005. Polyamines reduce salt-induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in virginia pine. Plant Growth Regulation, 46(1): 31-43.
Wahba, H., Motawe, H., and Ibrahim, A. 2015. Growth and chemical composition of Urtica pilulifera plant as influenced by foliar application of some amino acids. Journal of Materials and Environment Science, 6(2): 499-506.
Yuan, S. and Lin, H.H. 2008. Minireview: role of salicylic acid in plant abiotic stress. Zeitschrift für Naturforschung C, 63(5-6): 313-320.
