Ecophysiological acclimation and salinity amelioration of soil Cyanobacterium Anabaena sp. FS 76 collected from oil polluted regions under ccombined effects of salinity and extremely limited irradiances

Subject Areas : Genetic

Shadman Shokravi ¹ ( Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran )
Elahe Kiaei ² ( Young Researchers and Elite Club, Islamic Azad University, Gorgan Branch, Gorgan, Iran )
Afsane Pakzad ³ ( Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran )
Hamide Sadat Amirlatifi ⁴ ( Young Researchers and Elite Club, Islamic Azad University, Gorgan Branch, Gorgan, Iran )

Received: 2015-08-30 Accepted : 2016-04-24 Published : 2016-05-21

Keywords: Cyanobacteria, Ecophysiology, Anabaena sp.FS 76, Petroleum, Salinity amelioration,

Abstract :

The issue of salinity stress caused by oil extraction has been one of the major environmental issues of the country in the last decade. Biological agents in oil-contaminated areas can be considered the most efficient methods. The aim of this study was to reduce salinity by cyanobacteria isolated from oil contaminated areas. Soil cyanobacterium Anabaena sp. FS 76 were collected from oil polluted areas of southern Iran (Khuzestan province) and were studied ecophysiologically for acclimation ability at combination of different salinities (up to 1%) and extremely limited irradiances (2 μmol quanta.m-2S-1) at laboratory conditions. Samples were collected from oil polluted soils at different stations of Khuzestan province (Khark) for one year. Anabaena sp.FS 76 showed common strain and was selected for next steps after preliminary identification. Salinity treatments included Salt-free medium and BG011 medium with 0.25, 0. 5, 0.75, and 1% sodium chloride which were applied before and after limited irradiance treatments. Analysis involved growth analysis, Pigments (phycocyanin, allophycocyanin, phycoerythrin) and protein and sugar content assays. Results showed that the maximum growth rate may be observed at 1% salinity. At this range of salinity. 1% NaCl not only may not decrease total sugar and protein content but also cause significant increase. In vivo absorption spectra support the presence of central and rod sections of phycobilisome. This strain has salinity amelioration ability at the laboratory conditions. This potentiality reaches to highest degrees of salinity especially at 1% probability level. The results showed examples of environmental physiology and biotechnology powerful is for future studies.

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