Antibacterial activity of alcoholic and aqueous extracts of Echinochloa crus-galli L. against bacteria in vitro

Subject Areas : Genetic

سیدمسعود HashemiKarooyi ¹ ( Department of Microbiology, Islamic Azad University, Tonkabon Branch )
آیت الله Nasrollahi omran ² ( Faculty of Medical Science, Islamic Azad University, Tonkabon Branch )
H.R Pordeli ³ ( دانشگاه آزاد اسلامی واحد گرگان )
عیسی Gholampoor Azizi ⁴ ( Department of Microbiology, Islamic Azad University, Babol Branch )

Received: 2008-07-23 Accepted : 2008-12-05 Published : 2009-06-15

Keywords: antibacterial effect, Echinochloa crus-galli L, Aqueous and alchoholic extract,

Abstract :

The antagonistic effects that plants have on different micro organisms can be used to protect microorganism from the environment. The Echinochloa crus-galli with a universal growth is found in paddy fields in the North of Iran, named Soroof, in the weed form. In this research,different methanolic, ethanolic and aqueous extract have been provided from Echinochloa crus-galli and their effects on gram positive & gram negative bacteria growth using disk diffusion, well diffusion, plate count, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. It concluded that different types of methanolic, ethanolic& aqueous extract inhibited Staphylococcus aureus and Bacillus cereus growth while did not have any effect on Escherichia coli and Pseudomonas aeroginosa growth.

References:

کوچکی، ع.، رحیمیان، ح., نصیری محلاتی، م. (1373). اکولوژی علف‌های هرز. انتشارات دانشگاه مشهد. 320ص.

ولاگ، ژ.، استودولا، و. ترجمه زمان ساعد. (1374). گیاهان داروئی. انتشارات ققنوس تهران. 750 ص.

Abdumoniem. M.A.S (2006): Antifungal activity of some Saudi plant used in traditional medicine. Asian Journal of Plant Science. 5(5). pp 907-909.

Aderotimi. B and Samuel. A. (2006): phytochemical screening and antimicrobial assessment of Abutilon mauritianum, Bacopa monifera and Datura stramonium. Biokemistri. vol.18. No.1: 39-44.

Albert J. fisher.; Bayer D.E.; Carriere M.D.; Ateh C.M.; Yim K-O.(2000). Mechanisms of Resistance to Bispyribac- sodium in an Echinichloa phyllopogon Accession. Pesticide Biochemistry and Physiology. 68(3): 156-165.

Berrin O., Mustafa A., Hkay O. and Taner K. (2005). Antibacterial, antifungal and antiviral activities of the lipophylic extracts of Pistacia vera. Microbiological Research. 160(2):159-164.

Cheah L.H., Tate K. G., Hunt A. W., desilva N.(1993). Decreased sensitivity of peach leaf curl (Taphrina deformans) to copper fungicides in Hawkes Bay. Proceeding of the 46^th NZ plant protection conference, Waitangi city, New Zealand: 15-17.

Chung I.M., T. Kim and S.H. Kim,. (2002). Screening of allelochemicals on baryarolgrass (Echinochloa crus-galli) and identification of potentially allelopathic compounds from rice (oryza sative) variety hull extracts. Crop protection. 21(10): 913-920.

Ellen JO. Baron, Lancer R. Peterson and Sydney M. Finegold. (1994). Diagnostic Microbiology. Ninth Edition. Mosby – Year book. Inc.

Krol W., Scheller S., Shani J., Piets G. and Ozaba Z.(1993). Synergistic effect of ethanolic extract of propolis and antibiotic on the growth of staphylococcus aureus. Arzeimitte for schuug 43: 607-609.

Marcucci M. C. (1995). Propolis; chemical composition, biological properties and therapeutic activity, Apidologie. 26: 83-99.

Scazzocchia F., F.D. D'Auriaa, D. Alessandrinia. (2006). Multifacterial aspect of antimicrobial activity of propolis; Micribiological Research. 161(4): 327-333.

Tran Dang, X.O.Y, Chikara, J., Eiji, T. (2003). Kava root (piper methysticum L.) as a potential nature herbicid and fungicide. Crop Protection. 22 (6): 873-881.