Examining of symbiotic effects of Mycorrhiza (Glomus mosseae) with bread wheat (Triticum aestivum L.) Under drought stress conditions
Subject Areas : TensionFarzaneh Etemadi 1 , Sara Saadatmand 2 , Rahim Ahmadvand 3 , Iraj Mehregan 4 , Amin Azadi 5
1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Seed and Plant Breeding Research Institute, Tehran, Iran.
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
5 - Department of Agriculture, Yadegar Imam Branch, Islamic Azad University, Shahr-e Ray, Iran.
Keywords: Antioxidant enzymes, Drought stress, soluble carbohydrates, Bread wheat, Mycorrhiza,
Abstract :
In order to investigate the effect of the symbiosis of arbuscular mycorrhizal fungi and drought stress on the growth, yield, chlorophyll content and morphophysiological characteristics of Chamran wheat seedlings (Triticum aestivum L.), a research in the form of a factorial experiment in the form of a randomized complete block design and in four replications in 2016 It was carried out in the research laboratory of Islamic Azad University, Science and Research Unit, Tehran. The experimental factors included two levels of drought stress (agricultural capacity and 50% of agricultural capacity) and two levels of mycorrhizal inoculation (inoculation and no mycorrhizal inoculation). The evaluated traits included chlorophyll, carotenoid, flavonoid, anthocyanin, proline, soluble carbohydrate, membrane lipid peroxidation, phenol, total protein, catalase, and superoxide dismutase and ascorbate peroxidase enzymes. The results of variance analysis of the data showed a significant decrease in drought stress of photosynthetic pigments, flavonoids, anthocyanins and soluble sugars at the level of 1% and a significant increase in phenol, proline and total protein and a decrease in the content of malondialdehyde in wheat plants. Also, the studies revealed that the activities of catalase, superoxide dismutase and ascorbate peroxidase enzymes increased under drought stress. In general, the application of mycorrhizal treatment increased and improved the growth and promoted the activity of antioxidant enzymes. The result is that in water shortage conditions, the use of mycorrhizal fungi by creating changes in physiological processes and activating the antioxidant defense system and biochemical changes, gives the plant the possibility of better adaptation under dry conditions and the effective role of this fungus in increasing growth and resistance to stress. It means bread in the wheat plant.
Abbaszadeh, B., Sharifi Ashourabadi, E., Lebaschi, M.H., Naderi Hajibagher kandi, M. and Moghadami, F. (2008). The Effect Of Drought Stress On Proline Contents, Soluble Sugars, Chlorophyll And Relative Water Contents Of Balm (Melissa Officinalis L.). Iranian Journal of Medicinal and Aromatic Plants and Research. 23 (38):504-513.
Ghabooli, M., Khatabi, B., Ahmadi, F.S., Sepehri, M., Mirzaei, M., Amirkhani, A., Jorrín-Novo, J.V. and Salekdeh, G.H. (2013). Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley. Journal of Proteomics. 94: 289-301.
Aebi, H. (1984). Catalase in Vitro. Methods Enzymology, 105, 121-126. http://dx.doi.org/10.1016/S0076-6879(84)05016-3.
Ahanger, M., Tyagi, S., Wani, M. and Ahmad, P. (2013). Drought tolerance: role of organic osmolytes, growth regulators, and mineral nutrients. Physiological Mechanisms and Adaptation Strategies in Plants under Changing Environment, 11: 25-55.
Alam, Sh., Kamei, Sh. and Kawai, Sh. (2003). Amelioration of Manganese Toxicity in Young Rice Seedlings with Potassium. Journal of Plant Nutrition. 26(6):1301–1314. https://doi/abs/10.1081/PLN-120020372.
Alvarez M, Gieseke A, Godoy R.H. and Artel, S. (2006). Surface-bound phosphatase activity in ectomycorrhizal fungi: a comparative study between a colorimetric and a microscope-based method. Biology and Fertility of Soils. 42: 561–568.
Arazmjo A., Heidari M.and Ghanbari A.(2010). The Effect Of Water Stress And Three Sources Of Fertilizers On Flower Yield, Physiological Parameters And Nutrient Uptake In Chamomile (Matricaria Chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants and Research. 25(4):482 - 494.
Asadi Kavan, Z.h., Ghorbanli, M. and Sateei, A. (2010). The effect of drought stress and exogenous ascorbate on photosynthetic pigments, flavonoids, phenol compounds and lipid peroxidation in Pimpinella anisum L. Iranian Journal of Medicinal and Aromatic Plants and Research. 25(4): 456-69.
Bates, L. S., Waldren, R. P. and Teare, I. D. (1973). Rapid Determination of Free Proline for Water-Stress Studies. Plant and Soil .39 (1): 205–7. https://doi.org/10.1007/BF00018060.
Baum, C.W., Tohamy, E. and Gruda, N. (2015). Increasing the Productivity and Product Quality of Vegetable Crops Using Arbuscular Mycorrhizal Fungi: A Review. Scientia Horticulturae. 187:131–41. https://doi.org/https://doi.org/10.1016/j.scienta.2015.03.002.
Beyer., F. and Fridovich, I. (1987). Assaying for Superoxide Dismutase Activity: Some Large Consequences of Minor Changes in Conditions. Analytical Biochemistry. 161(2):559–566. https://doi.org/https://doi.org/10.1016/0003-2697(87)90489-1.
Bors, W. (1996). Flavonoids and Polyphenols: Chemistry and Biology. Handbook of Antioxidants 409.
Bowles, T.M., Jackson, L.E. and Cavagnaro, T.R. (2018). Mycorrhizal Fungi Enhance Plant Nutrient Acquisition and Modulate Nitrogen Loss with Variable Water Regimes. Global Change Biology. 24(1): e171–82. https://doi.org/10.1111/gcb.13884.
Bradford, M. M. (1976). A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry. 72:248–54. https://doi.org/10.1006/abio.1976.9999.
Brenchley, R., Spannag M., Pfeifer, M., Barker, G., D’Amore, R., Allen, A.M. and McKenzie, N . (2012). “Analysis of the Bread Wheat Genome Using Whole-Genome Shotgun Sequencing. Nature. 491 (7426): 705–10. https://doi.org/10.1038/nature11650.
Brundrett, M. C. and Tedersoo, L. (2018). Evolutionary History of Mycorrhizal Symbioses and Global Host Plant Diversity. New Phytologist. 220(4):1108–15. https://doi.org/10.1111/nph.14976.
Cho, U.H. and Park, G.O. (2000). Mercury-Induced Oxidative Stress in Tomato Seedlings. Plant Science.156 (1): 1–9. https://doi.org/10.1016/j.indcrop.2019.111553
Demir, S. (2005). Influence of Arbuscular Mycorrhiza on Some Physiological Growth Parameters of Pepper. Turkish Journal of Biology .28 (2–4): 85–90.
Dixit, V., Pandey,V. and Shyam,R. (2001). Differential Antioxidative Responses to Cadmium in Roots and Leaves of Pea (Pisum Sativum L. Cv. Azad). Journal of Experimental Botany. 52(358):1101–1109. https://doi.org/10.1093/jexbot/52.358.1101.
Ebrahimzadeh, M.A, Hosseinimehr, S.J. and Hamidinia,A. (2008). Antioxidant and Free Radical Scavenging Activity of Feijoa Sallowiana Fruits Peel and Leaves.
Farooq, M., Wahid, A., Kobayashi, N.,Fujita, D. and Basra, S. M. A. (2009b) .Plant drought stress: mechanisms and management. Agronomy for Sustainable Development. 29: 185–212.
Fazeli, F., Ghorbanli,M. and Niknam, A. (2007). Effect of Drought on Biomass, Protein Content, Lipid Peroxidation and Antioxidant Enzymes in Two Sesame Cultivars. Biologia Plantarum. 51(1):98–103. https://doi.org/10.1007/s10535-007-0020-1.
Fecht, C., Marion, M., Maier, P. and Horst, W.J. (2003). Apoplastic Peroxidases and Ascorbate Are Involved in Manganese Toxicity and Tolerance of Vigna Unguiculata. Physiologia Plantarum. 117 (2): 237–44. https://doi.org/10.1034/j.1399-3054.2003.00022.x
Ghabooli, M. (2014). Effect of Piriformospora indica inoculation on some physiological traits of barley (Hordeum vulgare) under salt stress. Chemistry of Natural Compounds, 50(6): 1082-1087.
Gholamhoseini, M.A ., Dolatabadian, A., Jamshidi, E. and Khodaei-Joghan., A. (2013). Effects of Arbuscular Mycorrhizal Inoculation on Growth, Yield, Nutrient Uptake and Irrigation Water Productivity of Sunflowers Grown under Drought Stress. Agricultural Water Management. 117:106–14. https://doi.org/https://doi.org/10.1016/j.agwat.2012.11.007.
Hagar, H., Ueda, N. and Shah, S.V. (1996). Role of Reactive Oxygen Metabolites in DNA Damage and Cell Death in Chemical Hypoxic Injury to LLC-PK1 Cells. American Journal of Physiology-Renal Physiology. 271 (1): F209–15. http://dx.doi.org/10.1152/ajprenal.1996.271.1.F209.
Hamed, A., Akbari, Gh.A., Khoshkholgh Sima, N. A., Shirani Rad, A.H., Jabbari, H. and Tabatabaee, S.M. (2014). Evaluation of the agronomic characteristics and some physiological traits of canola varieties under drought stress. Environmental Stresses in Crop Sciences. 7(2): 155-171.
Hatamvand, M., Hasanloo, T., Dehghan Nayeri, F., Shiranirad, A.H., Tabatabaei, S.A. and Hosseini, S.M. (2014). Evaluation of some physiological and biochemical indices of canola cultivars in response to drought stress. Environmental Stresses in Crop Sciences. 7 (2): 173-185.
Heath, R.L. and Pacher, L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometery of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125 (1): 189-198.
Huang ,H., Ullah , F., Zhou ,D.Z., Yi, M . and Zhao,Y.(2019). Mechanisms of ROS Regulation of Plant Development and Stress Responses. Front. Plant Science. https://doi.org/10.3389/fpls.2019.00800.
Jahromi, F., Aroca, R. Porcel, R.and Ruiz-Lozano, J.M. (2008). Influence of salinity on the in vitro development of Glomus intraradices and on the in vivo physiological and molecular responses of mycorrhizal lettuce plants. Microbial Ecology. 55: 45–53.
Jiang, M. and Zhang, J. (2001). Effect of Abscisic Acid on Active Oxygen Species, Antioxidative Defence System and Oxidative Damage in Leaves of Maize Seedlings. Plant and Cell Physiology. 42 (11): 1265–73.
Jones, M.M., Osmond, C.B. and Turner, N.C. (1980). Accumulation of solutes in leaves of sorghum and sunflower in response to water stress. Australian Journal of Plant Physiology. 7: 193-205.
Jung, S. (2004). Variation in Antioxidant Metabolism of Young and Mature Leaves of Arabidopsis Thaliana Subjected to Drought. Plant Science. 166 (2): 459–66.
Kapoor, R., Giri, B. and Mukerji, K.G. (2004). Improved growth and essential oil yield and quality in (foeniculum vulgare Mill.) on mycorrhizal inoculation supplemented with P fertilizer. Journal of Biotechnology. 93: 307-311.
Khalid, Kh.A. (2006). Influence of water stress on growth, essential oil, and chemical composition of herbs (Ocimum sp.). Agrophysics. 20:289-296.
Khalil, N., Fekry, M., Bishr, M., Zalabani, S. and Salama, O.( 2018). Foliar Spraying of Salicylic Acid Induced Accumulation of Phenolics, Increased Radical Scavenging Activity and Modified the Composition of the Essential Oil of Water Stressed Thymus Vulgaris L. Plant Physiology and Biochemistry. 123: 65–74. https://doi.org/10.1016/j.plaphy.2017.12.007.
Kheira, K., Benchaben, H., Adiba, B.D, Nadira, A. and Abbassia, A. (2015). Seasonal variations of ballotahirsutabenth flavonoids of tessala mount of the prefecture of SidiBel-Abbes (Western Algeria). Biochemistry and Molecular Biology Letters. 1(1): 001-006.
Koc, E., İslek, C. and Üstün, A.S. (2010). Effect of cold on protein, proline, phenolic compounds and chlorophyll content of two pepper (Capsicum annuum L.) varieties. Gazi University Journal of Science. 23(1): 1-6.
Koochaki, A., Nassiri mahalati, M. and azizi, G. (2005). The effects of water stress and defoliation on some of quantitative traits of Zataria multiflora, Ziziphora clinopodioides, Thymus vulgaris and Teucrium polium. Iranian Journal of Field Crops Research. 1(2):89-105.
Krizek, D. T., Britz. J.S. and Mirecki,R.M. (1998). Inhibitory Effects of Ambient Levels of Solar UV-A and UV-B Radiation on Growth of Cv. New Red Fire Lettuce. Physiologia Plantarum. 103(1):1–7. https://doi.org/10.1034/j.1399-3054.1998.1030101.x.
Kusvuran, S. (2011). Effects of drought and salt stresses on growth, stomatal conductance, leaf water and osmotic potentials of melon genotypes (Cucumis melo L.). African Journal of Agricultural Research. 7 (5): 775-781.
Lewis, J.D., Olszyk, D. and Tingey, D.T. ( 1999). Seasonal Patterns of Photosynthetic Light Response in Douglas-Fir Seedlings Subjected to Elevated Atmospheric CO2 and Temperature. Tree Physiology. 19 (4–5): 243–52.
Li, J., Meng, B.O., Chai, H., Yang, X., Song, W., Li, S., Lu, AO., Zhang,T. and Sun,W.( 2019). “Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in C3 (Leymus Chinensis) and C4 (Hemarthria Altissima) Grasses via Altering Antioxidant Enzyme Activities and Photosynthesis.” Frontiers in Plant Science 10 (April): 1–12. https://doi.org/10.3389/fpls.2019.00499
Lichtenthaler., H. K. (1987). Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods in Enzymology. 148:350–82. https://doi.org/https://doi.org/10.1016/0076-6879(87)48036-1
Ma, Q., Niknam, S.R. and Turner, D.W. (2006). Responses of osmotic adjustment and seed yield of Brassica napus and B.juncea to soil water deficit at different growth stages. Australia Journal of Agricultural Research. 57(2): 221-226.
Manankina, E. E., SMel’nikov, S.S., Budakova, E.A. and Shalygo, E.A. (2003). Effect of Ni 2+ on Early Stages of Chlorophyll Biosynthesis and Pheophytinization in Euglena Gracilis.” Russian Journal of Plant Physiology. 50 (3): 390–94
Mathur., S.R. and Jajoo,A. (2019). Arbuscular Mycorrhizal Fungi (AMF) Protects Photosynthetic Apparatus of Wheat under Drought Stress. Photosynthesis Research. 139(1–3):227–38. https://link.springer.com/article/10.1007/s11120-018-0538-4
Mazarie, A., Mousavi-nik, S. M., Ghanbari, A. and Fahmideh, L. (2019). Effect of titanium dioxide spraying on physiological characteristics of sage (Salvia officinalis L.) under water stress. Environmental Stresses in Crop Sciences. 12(2): 539-553.
Miller, G., Suzuki, N. and Ciftci Yilmaz, S. (2010). Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Journal of Plant Cell and Environment, 33: 453-467.
Mittler, R. (2002). Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science. 7 (9): 405–10. https://doi.org/10.1016/S1360-1385(02)02312-9.
Moucheshi, A., Heidari, B. and Assad, M.T.(2012). Alleviation of Drought Stress Effects on Wheat Using Arbuscular Mycorrhizal Symbiosis. International Journal of Agriculture Science. 2 (1): 35–47.
Nakano,Y. and Asada, K. (1981). Hydrogen Peroxide Is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts. Plant and Cell Physiology. 22 (5): 867–80.
Nalimova, A.A., Popova, V.V., Tsoglin, L. A. and Pronina, N.A . (2005). The Effects of Copper and Zinc on Spirulina Platensis Growth and Heavy Metal Accumulation in Its Cells. Russian Journal of Plant Physiology 52 (2): 229–34.
Ouzounidou, G. and Ilias, I. (2005). Hormone-Induced Protection of Sunflower Photosynthetic Apparatus against Copper Toxicity. Biologia Plantarum. 49 (2): 223.
Pandey, N. and Sharma, C.P. (2002). Effect of Heavy Metals Co2+, Ni2+ and Cd2+ on Growth and Metabolism of Cabbage. Plant Science. 163 (4): 753–58.
Panhwar, Q.A., Amanat, A., Naher, U.A. and Memon, M.Y. (2019). Chapter 2 - Fertilizer Management Strategies for Enhancing Nutrient Use Efficiency and Sustainable Wheat Production.” In , edited by Sarath Chandran, M R Unni, and Sabu B T - Organic Farming Thomas, 17–39. Woodhead Publishing. https://doi.org/https://doi.org/10.1016/B978-0-12-813272-2.00002-1.
Prasad, S.M. and Zeeshan, M. (2005). UV-B Radiation and Cadmium Induced Changes in Growth, Photosynthesis, and Antioxidant Enzymes of Cyanobacterium Plectonema Boryanum. Biologia Plantarum. 49(2): 229.
Rabino, I. and Mancinelli, A.L. (1986). Light, Temperature, and Anthocyanin Production. Plant Physiology .81 (3): 922–24. https://doi.org/10.1104/pp.81.3.922
Rokni, N. and Mohammadi Goltapeh, E. (2011). Diversity of Arbuscular Mycorrhizal Fungi Associated with Common Sugarcane Varieties in Iran. Journal of Agricultural Science and Technology. 7: 1017–22.
Sakihama, Y., Cohen, M.F., Grace, S.C. and Yamasaki, H. (2002). Plant Phenolic Antioxidant and Prooxidant Activities: Phenolics-Induced Oxidative Damage Mediated by Metals in Plants. Toxicology. 177 (1): 67–80.
Sntander, C., Aroca , R., Manuel Ruiz-Lozano, J., Olave,J., Cartes, P., Borie, F. and Cornejo, P. (2017) . Arbuscular Mycorrhiza Effects on Plant Performance under Osmotic Stress. Mycorrhiza. 27 (7): 639–57.
Sahabivand, S., Parvaneh, A. and Aliloo, A.A. (2017). Root endophytic fungus Piriformospora indica affected growth, cadmium partitioning and chlorophyll fluorescence of sunflower under cadmium toxicity. Ecotoxicology and Environmental Safety. 145: 496-502.
omogyi and Michael. (1952). Notes on Sugar Determination. Journal of Biological Chemistry. 195: 19–23.
Stearns, J. C., Shah, S., Greenberg, B. M., Dixon, D. G. and Glick, B. R. (2005). Tolerance of Transgenic Canola Expressing 1-Aminocyclopropane-1-Carboxylic Acid Deaminase to Growth Inhibition by Nickel. Plant Physiology and Biochemistry. 43 (7): 701–8.
Tajmmlian, M., Iran Nejad parazi, M.D., Malekinejad, H., Rad, M.H. and Sodaie zadeh, H. (2012). Effect of low stress on some physiological parameters of plant (Fortuynia bungei Boiss). Journal of Genetics Research and Plant Breeding, 20: 273-283.
Takahama, U. and Oniki, T. (1997). A Peroxidase/Phenolics/Ascorbate System Can Scavenge Hydrogen Peroxide in Plant Cells. Physiologia Plantarum .101 (4): 845–52.
Tattini, M., Galardi, C., Pinelli,P., Massai, R., Remorini, D. and Agati, G. (2004). Differential Accumulation of Flavonoids and Hydroxycinnamates in Leaves of Ligustrum Vulgare under Excess Light and Drought Stress. New Phytologist .163 (3): 547–61.
Xu, C., Yin, Y., Cai, R., Wang, P., Ni, Y., Guo, J., Chen, E., Cai, T., Cui, Z . and Liu,T. (2013). Responses of Photosynthetic Characteristics and Antioxidative Metabolism in Winter Wheat to Post-Anthesis Shading. Photosynthetica. 51 (1): 139–50.
