Effect of Explant Type, Plant Growth Regulators and LED lights on the Establishment and Propagation of ZZ Plant (Zamioculcas zamiifolia)

Subject Areas : Tissue culture

Fatemeh Mousali ¹ (Horticultural Sciences - Tabriz University Faculty of Agriculture - Tabriz - Iran)
Mansour Matloobi ² (Department of Horticultural Sciences. Tabriz University Faculty of Agriculture - Tabriz - Iran)
Alireza Motallebi Azar ³ (Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.)
mohammadvali habibi ⁴ (Horticultural Sciences - Tabriz University Faculty of Agriculture - Tabriz - Iran)
Asghar mohammadi ⁵ (Department of Horticultural Sciences. Tabriz University Faculty of Agriculture - Tabriz - Iran)

Received: 2023-06-19 Accepted : 2024-07-13 Published : 2024-07-19

Keywords: BAP, NAA, Blue light, Light quality, Tissue culture,

Abstract :

Zamofilia is one the most valuable foliage plants which is commonly propagated using vegetative methods. This technique requires a great number of mother plants to provide adequate cuttings for commercial propagation which hardly seem to meet the most economical expectations. However, due to the slow growth habit of this plant, even under ideal growing conditions, its commercial and mass production are limited by some species-related characteristics. Therefore, developing an improved method of micropropagation which lead to a fast and reliable mass propagation technique may overcome most of these limitations especially at commercial scale. In this regard, this research was conducted and aimed to introduce an efficient micropropagation method in in-vitro condition for rapid propagation of this plant. Two different concentrations of BAP (1 and 2 mg L-1) and NAA (0 and 0.1 2 mg L-1 ) were applied on the explants taken from one of the three different positions of the leaflets (i.e., lower, middle and upper parts) cultured in MS medium and treated with blue (460 nm) or red (660 nm) light supplied by LED luminaries The results showed that the best treatment for callus and protocorm formation with the maximum stimulation (93.75%) and (66.66%) was the application of 2 mg/L BAP along with 0.1 mg/L NAA in The light was red. Callus production occurred in cut surfaces by 90% under blue and 85% under red light, while these lights induced protocorm indirectly by 80% and 90% respectively, leaving the rest to be regenerated by directly. Blue and red light induced protocorm emergence by 95% and 97% respectively from the cut surface and the parts around the cut location were responsible for the emergence of the remained protocorms. The highest rate of regeneration of protocorms (95%) in the treatment of 0.1 mg/liter NAA .